Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 337,543 T→C 13.7% S256P (TCC→CCC)  yahF → putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913337,5430TC13.7% 231.1 / 3.1 102S256P (TCC→CCC) yahFputative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (40/44);  new base C (3/13);  total (43/59)
Fisher's exact test for biased strand distribution p-value = 5.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGATTGCGGATGTGCGTGCG  >  NC_000913/337311‑337779
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGGGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCCGGGTGCTGGGAAACGAACCG‑CAAAATGAAATCATTGCGC‑CTATCTCCaaa                                                                                                                                                                                                                                          >  1:218555/1‑238 (MQ=255)
cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑ACTGGGCGAGAAAATCGGCGGCCT‑GATGATGCTCGGCG‑CCATCGGGGTGCCGGAAAACGATACG‑CCAAATGAAAACATTGCGC‑TTATCTCCcaa                                                                                                                                                                                                                                          >  1:401972/1‑238 (MQ=255)
     cgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACACCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACcgcc                                                                                                                                                                                                                                     <  1:162896/238‑1 (MQ=255)
      gcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACcgcct                                                                                                                                                                                                                                    <  1:200379/238‑1 (MQ=255)
        ctggTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACcgcctgc                                                                                                                                                                                                                                  <  1:204188/235‑1 (MQ=255)
         ctGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACcgcctgcgc                                                                                                                                                                                                                                <  2:1264544/239‑1 (MQ=255)
          tGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCGA‑CCCTAGCGGGAAAATCGGCGGGCT‑GGTGGTGCCCTGCG‑GCACACGGGTGCTGGGAAACGGACCC‑ACGACCGAAATCAATTCGC‑GTTTCTCCAAaccacccccgc                                                                                                                                                                                                                                >  2:988681/1‑233 (MQ=255)
          tGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGGGAAAAACGGCGGCCT‑GGTGATGCTCGGCG‑CCCTCGGGGTGCTGGGAAAAGAACCC‑CCAACTGAAAACATTGGGC‑TTTTCCTCAAACcgccccgcc                                                                                                                                                                                                                                >  2:935585/1‑233 (MQ=255)
               tGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGCGACAATCGGGGGCCT‑TGTGATGGCCGCCG‑CCATCGGGGTGCTTGAGAAAGACCCC‑CCAAAAGACATCCTTGAGC‑TTTTTTCCCAAAcgcctcccgcgcccg                                                                                                                                                                                                                          >  2:66389/1‑229 (MQ=255)
                ggTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGt                                                                                                                                                                                                                        <  1:511919/239‑1 (MQ=255)
                 gTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCGAGCCGGGGGGGGAAAAAAGG‑GGCCTCGAGGA‑GCTCCACGACCAACGGGGTCGTGGAAAAGGAGCGC‑GACACGGAAAGCATTGCG‑‑TTATTCCCCAACcgcccgcgccctccgtc                                                                                                                                                                                                                        >  2:1380675/1‑232 (MQ=255)
                   aaCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGc                                                                                                                                                                                                                     <  1:403516/239‑1 (MQ=255)
                    aCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCCTCGGGATGCTGGGAAACGATCCG‑CAAAATGAAAACATTGTGC‑TTATCTCCAAACCGCCCGCGCCTG‑CGGTGGc                                                                                                                                                                                                                     >  1:138373/1‑238 (MQ=255)
                      gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCGA‑ACCGAGCGAGGAAAACCGCGGCCT‑GATGGTGGTCGCCG‑CCCTCCGGGTGCTGGGAAAAGCTCCC‑CCAACCGACATCACTTCGC‑TTTTCTCCAAACCCCCCGCGCCCG‑CCGGGGccc                                                                                                                                                                                                                   >  2:1247351/1‑238 (MQ=255)
                      gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCCA‑CCTGAGGGGGGAAATCGGCGGCCT‑GGTGATTCTCCGCG‑GCATCGGGGTGCCGGAAAACGGACCG‑CAAACTGAAAATATT‑CGCGTTATTTCCCAACcgcacgcgccccccgtgggcc                                                                                                                                                                                                                    >  2:811053/1‑227 (MQ=255)
                        tgtggttggcgGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTGGGGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  2:1103733/231‑1 (MQ=255)
                        ggTGCGTCGCGGCAACATCGGTTTTGTTGGCGCATCCGGCACCGGAAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  2:722290/238‑1 (MQ=255)
                        cgcgggtggcgGCAAGATCGGTATTGTGGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCGGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  2:179577/231‑1 (MQ=255)
                        cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGGGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  2:449914/239‑1 (MQ=255)
                        cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGGGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  1:327017/239‑1 (MQ=255)
                        cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAGCCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  1:1242744/239‑1 (MQ=255)
                        cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  1:946267/239‑1 (MQ=255)
                        cGTGCGGCGGGGGAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCTTTGCGCAACTGATTGGCCCCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  2:1384391/239‑1 (MQ=255)
                         gttagTCGCGGCATCGGCGGTATTGTTGGCCAATCCGGCAGCGGCAGTCAGAAGTATAAGGCCAAAAATGATGAACTGGGCGCCGGCCTCTCGCAAGTGATTGGCGCCGGCGGGTGCGG‑GCTGACCGAGAAAATCGGCGGCCT‑GATAATGCCCGACG‑CCTTCGGGATGCTGGAAATCGATCGG‑CAAACCAAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  2:565961/234‑1 (MQ=255)
                          tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCGA‑ACGGAGCGGGAAAATCGGCGGCCT‑GGTGGTGCTCGGCG‑CCCTCGGGGTGCTGGAAAACGAACCG‑CAAACTGAAATAATTGTGC‑TTATCTCCCAACCCCCTGCGCCTC‑CGGGGGgcccccaa                                                                                                                                                                                                              >  2:1097106/1‑234 (MQ=255)
                          tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGGGGGAGAAAAACGGCGGCCT‑GGTGGTGCTCCGCG‑CCCTCCGGGTGCTGGAAAAAGATCCGCCAAACGGAAA‑CATTGCGC‑TTTTTTCCAAACCCCCTAGGCCCGCCGGGGGCCCCCaa                                                                                                                                                                                                               >  2:1390497/1‑239 (MQ=255)
                          tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGGTGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCCCCCGCGCCCG‑CGGGGGCCCGCcaa                                                                                                                                                                                                              >  1:550272/1‑239 (MQ=255)
                          tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCCGAGCGAGAAAAACGGCGGCCT‑GATGATGCTCGCCG‑CCATCCGGGTGGCGGGAAACGAGCCC‑CCAAATGTAATCATTTCGC‑CTATCTCCCAAACGCCCGCGCCTG‑CGGGGGCCCGCaac                                                                                                                                                                                                              >  2:188360/1‑238 (MQ=255)
                           gggccacgGCAACATCGGTGTTGTTGGCGCATACGGCACCGGCGGCCCGGAGTTGAGCGCCCGCATTCAGGAATTTGGCGCAGGCGTTTCGCAACTGTTCGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCTACA‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCTCGCa                                                                                                                                                                                                                <  2:1021807/230‑1 (MQ=255)
                           gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGGAAAACGGCGGCCT‑GGTGATGCCCGACG‑CCCTCGGGGTGCTGGGAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCCG‑CGGGGGCCCGCCAAg                                                                                                                                                                                                             >  1:636970/1‑239 (MQ=255)
                           gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCaaa                                                                                                                                                                                                              <  1:1390497/238‑1 (MQ=255)
                                gcgGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGGGCGGGAAAATCGGCGGCCC‑GGTTTTTCGCGACC‑CCCCCCGGATGCGGGGAAAAGCACCC‑CACACTGAAAACAATTCGC‑CTCTCTCCACACCCCCGCCGCCCG‑CCGGGGCCCCCCAAGCGCTg                                                                                                                                                                                                        >  2:818249/1‑239 (MQ=255)
                                                  ttgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCC‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCC‑CAAACTGAAATCATTGCGC‑TTTTCTCCCAACCCCCCGCGCCTT‑CGGTGGCCCGCCAAATGCTGGAACGTGCGCGcccccg                                                                                                                                                                                       >  1:31788/1‑236 (MQ=255)
                                                    ggCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCg                                                                                                                                                                                    <  1:1437772/239‑1 (MQ=255)
                                                     gcgcATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCg                                                                                                                                                                                    <  1:342043/238‑1 (MQ=255)
                                                       gcATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGGGA‑ACTGGAGGAGAAAAACAGCGGCCC‑GAGGATGCGCGACG‑CCATCGGGCGGGTGGGAAACGATCCG‑CAAACTGAAATCAATGCGCTTTACACCCAAACCCCCGCCCCCGG‑CGG‑GGCCCCCAAAAGGGTGGAGAGGGCGCGCGcccggcccca                                                                                                                                                                                 >  2:1013303/1‑231 (MQ=255)
                                                       gcATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCAGCGGCCT‑GGTGATGCTCGCCG‑CCACCCGGCTGGTGGAAAACGATCCG‑CAAACTGAAATCATTTCGC‑CTATCTCCAAAACCGCTGCGCCCG‑CGGTGGCCCGCAAAGTGCTGGAACATGCGCGcgcccgccccc                                                                                                                                                                                  >  2:57226/1‑235 (MQ=255)
                                                         acccggccccgcaaGTCGGGAGTGGGGCGTCCGCATTCAGGAATTGGGCGGCGCCGTTCCCCAACTGATTGGCACCGGCGGGCGCGA‑CCGGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCGGGAAAACGACCCG‑CAAACTAAAACCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGc                                                                                                                                                                               <  2:527087/237‑1 (MQ=255)
                                                         aTCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCCGAGCGGGAAAATCGGCGGGCT‑GATGATGCTCGCCG‑CCCCCGGGGTGCTGGGAAACGATCCC‑CAAAATGAAAACCTTTCGC‑CTTTCTCCAAACCGCCTCCCCCCC‑CGGGGGCCCCCAAAACGCTGGAACGTgcgcgcggcgccccccag                                                                                                                                                                                >  2:315641/1‑227 (MQ=255)
                                                          tCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                                                <  1:369692/205‑1 (MQ=255)
                                                               caccggcaGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGGGAAAATCGGCGGCCT‑GGTGGTGCCCGACG‑CCACCGGGGTGCTGGAAAAAGATCCG‑CAAACTGAAATACTTGCGC‑CTATCTCCCAACCCCCCGGCCCCG‑CCGTGGCCCGCCAAGGGCTGGGACGGGGGCCGCCCGGCCGCCAGCCggggg                                                                                                                                                                         >  2:780871/1‑239 (MQ=255)
                                                                    gcaGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GGTGATGCTCGACG‑CCATCGGGGTGCTGGGAAAAGATCCG‑CAAAACTAAATCATTGCGC‑TTATCTCCCAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTggg                                                                                                                                                                                                      >  2:369692/1‑204 (MQ=255)
                                                                                      tCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCCA‑CCTGAGCGAGAAAATCGGCGGCCT‑GGTGGTGCTCGACG‑CCCTCGGGAGGCTGGAAAACGATCCC‑CCCACTGCAATCCTTTCGC‑TTCTCTCCCCACCCCCCGTGCCTG‑CGGTGGCCCCCAAAGTTCTGGAACGGGCGCCGCCCCGCCGCCCGCCGGTGGCCGTCTGCTTCCTCGCTCGTCGc                                                                                                                                                  >  2:843284/1‑239 (MQ=255)
                                                                                        cGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGGGCGGGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCCTCGGGGTGCTGGAAAACGATCCG‑CAAACTGAAATCATTTCGC‑TTATCTCCAAACCGCCTTCGCCTG‑CGGGGGCCCGCCAAGTGCTGGGACGTGCGCCCGCCCGCCGCCAGCCGGGGGTCGTCTGCTTCCTCCATCGTGGCGa                                                                                                                                                >  2:554118/1‑239 (MQ=255)
                                                                                         caaTTCAGGAATGTGGCGGCGGCGTTTCGCACCTGATTGGCCCCGGCGGGCTCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCGGCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGaa                                                                                                                                               <  1:236191/237‑1 (MQ=255)
                                                                                                          gggggctgttCGCAACTGATGGGCACCGGGGGGCGAGA‑CCTGAGCGAGAAAGTCGGCGGCGT‑GAGTATGCTCGCCC‑CCATCGGGAGGCGGGAAAACGAACCG‑CAACCTAAAATCATTGCTC‑TTAGCGCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                                               <  2:463121/230‑1 (MQ=255)
                                                                                                          ggcggggTTTCGAAACTTATTGGCCCGGGGGGGCGCAA‑CGTGAGCAAGAAAAGCGGGGGCTG‑GAGGATGCGCGACG‑CAATGGGGAGGCGGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCAAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                                               <  2:283806/238‑1 (MQ=255)
                                                                                                          ggcggggTTCCGCAACTGATTGGCCCCGGGGGGCGGGA‑CCGGAGGGAGAAAATCGGCGGCCG‑GAGGAGGCCCGACG‑CCCTCGGGATGCTGGAAAACGACCCG‑CAAACGGAAATCATTGCGC‑TTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                                               <  2:550272/238‑1 (MQ=255)
                                                                                                          ggcggcgTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAAGCGGCGGCCT‑GTTGATGCCCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                                               <  1:246450/238‑1 (MQ=255)
                                                                                                          ggcggcgTTTCGCAACGGATTGGCCCCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGAGGCTCGCCG‑CCATCGGGATGCTGGAAACCGATCCG‑CAAACTGAAATCATTGCGC‑TTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                                               <  1:1507615/238‑1 (MQ=255)
                                                                                                            cggcgTTCCGCAACGGTGTGGCCCCGGCGGGCGCGA‑CCGGAGCGAGAAAATCGGCGGCCT‑GATGATGCCCGACG‑CCATGGGGATGCTGGAAACCGACCCG‑AAAACTAAAATAATTCCCC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAg                                                                                                                             <  2:55572/238‑1 (MQ=255)
                                                                                                                gTTTCGCCACTGATTGGCCCCGGGGGGCGCGT‑CCTGAGCAAGAAAACCGGCGGCCT‑GAGGATGCCCGCGG‑CCATGGGGATGCGGGAAACCGACCCG‑CAACCTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGc                                                                                                                         <  2:272905/238‑1 (MQ=255)
                                                                                                                    cgaaaCGGATGGGCACGGGCGGGCGCGA‑CCTGAGCGAGAAAATGGGCGGCCT‑GATGATGCTGGACG‑CCATCGGGATGCTGGAAAACGACCCG‑AAACCTGAAATCATTGCGC‑TTACCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAg                                                                                                                    <  2:138373/236‑1 (MQ=255)
                                                                                                                                            gcgA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGc                                                                                             <  1:942019/238‑1 (MQ=255)
                                                                                                                                             gcgcacggAGCGAGAAAACCGCGGGCCG‑TATGATTCTCGACG‑CCCTCGGGATGTTGGAAAACGACCCG‑CAAACTAAAATCATTGCGC‑TTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGc                                                                                             <  2:954911/231‑1 (MQ=255)
                                                                                                                                                    gggCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTaa                                                                                     <  1:365049/237‑1 (MQ=255)
                                                                                                                                                      gCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATTCTGGAAAACGATCCG‑CAACCTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTaaa                                                                                    <  1:182121/238‑1 (MQ=255)
                                                                                                                                                       cGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTaaaa                                                                                   >  1:970775/1‑238 (MQ=255)
                                                                                                                                                        gaaaaaaTCGGGGGCCT‑GATTGGGTGCGACG‑CCATCGGGATGCTGGAAAACGACCCG‑CAAACTGAAATCATTGCGC‑TTACCTCCAACCCGCCTGCGCCGG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGAGCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAg                                                                                  <  2:1143540/235‑1 (MQ=255)
                                                                                                                                                            aagtCGGCGGCCG‑GATGATGCTCGACA‑CCAGCGGGATGCGGGAAACCGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGTGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTg                                                                             <  1:843284/236‑1 (MQ=255)
                                                                                                                                                            aaaCCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAACCTGAAATCATTGCGC‑TTACCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCgg                                                                               <  1:935585/237‑1 (MQ=255)
                                                                                                                                                            aaaCCGGCGCCTG‑GATGTTGCTCGAGA‑CCACGGGGATGTTGGAAAACGACCCG‑AAAACTGAAATCCTTGCGC‑TTATCTCCAAGCCGCCTGCGCCTG‑CGGGGGCCCGCAGAGGGCGGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTg                                                                             <  2:482437/239‑1 (MQ=255)
                                                                                                                                                                                 cTCGGCG‑CCACGGGGATGCGGGAAACCGACCCG‑CAAACTGAAATCATTGCGC‑TTATCCCCAACCCGCCTGCGCCGG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGaaa                                                           <  2:24652/237‑1 (MQ=255)
                                                                                                                                                                                 cTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACa                                                         >  2:1382176/1‑239 (MQ=255)
                                                                                                                                                                                 cTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGGGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGCAACa                                                         >  1:228595/1‑239 (MQ=255)
                                                                                                                                                                                   cGACG‑CCAGCGGGATGCTGGAAAACGTCCCG‑AAAACTTAAACCATGGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGGGGCCCGAAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAgg                                                       <  1:848027/239‑1 (MQ=255)
                                                                                                                                                                                     aCG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGaa                                                     >  1:336088/1‑239 (MQ=255)
                                                                                                                                                                                     aCG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGaa                                                     >  2:1035042/1‑239 (MQ=255)
                                                                                                                                                                                      gcgcaaacGGGATGCTGAAAAGCGATCCG‑CAATTTGAAATCATTGGTC‑TTTTCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGa                                                      <  2:504261/231‑1 (MQ=255)
                                                                                                                                                                                          cgtggggATGAGGGAAACCGACCCG‑CAAACGCAAATCATTGCGA‑TTACCCCCAAACCGCCGGCGCCGG‑GGGTGGCCCGCAAAGTGCTGGAACGTGGGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAAtc                                                 <  2:218555/235‑1 (MQ=255)
                                                                                                                                                                                          cATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAACGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGaaaa                                                   >  1:1042316/1‑237 (MQ=255)
                                                                                                                                                                                           aTCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCTTTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGc                                                                                                                                                                                   >  2:230946/1‑108 (MQ=255)
                                                                                                                                                                                           aTCGGGATGCTGGAAAACGATCCG‑CAAACGGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGc                                                                                                                                                                                   <  1:230946/108‑1 (MQ=255)
                                                                                                                                                                                              gggATGCTGGACAACGATCCG‑CAAACTGAAATCTTGGCGC‑TAACCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGGCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGa                                             <  1:281062/239‑1 (MQ=255)
                                                                                                                                                                                               ggATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGa                                             <  1:811053/238‑1 (MQ=255)
                                                                                                                                                                                                gATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCCCCAAGCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGAc                                            <  1:66389/238‑1 (MQ=255)
                                                                                                                                                                                                gATGCGGGAAAACGATCCG‑AAACCTGAATCCATGGCGC‑TTATCCCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGGGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGAc                                            <  2:401972/238‑1 (MQ=255)
                                                                                                                                                                                                    cTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGTCGTGAAACTGGAAAATCTCGACCTGCa                                       >  1:1025752/1‑239 (MQ=255)
                                                                                                                                                                                                    cTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCa                                       >  2:1226392/1‑239 (MQ=255)
                                                                                                                                                                                                     tgaaaaaCGACCCG‑CACACGGAAAACAGGCCCC‑TTATCCCCCACCCGCCTGCGCATG‑CGGTGGCCCGCAAAGTGCTGGACCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCa                                       <  2:1066247/235‑1 (MQ=255)
                                                                                                                                                                                                     tGGAAAACGACCCG‑AAAGCGGAAACCATGGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGGGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCa                                       <  1:757244/238‑1 (MQ=255)
                                                                                                                                                                                                      ggAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCAt                                      >  2:207707/1‑238 (MQ=255)
                                                                                                                                                                                                      ggAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCAt                                      >  1:332707/1‑238 (MQ=255)
                                                                                                                                                                                                              aTCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGcc                                                                                                                                          <  2:1206886/130‑1 (MQ=255)
                                                                                                                                                                                                              aTCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGcc                                                                                                                                          >  1:1206886/1‑130 (MQ=255)
                                                                                                                                                                                                              aTCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTaa                              >  1:680810/1‑238 (MQ=255)
                                                                                                                                                                                                               tCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGc                                                                                                                                                                                                          >  2:140571/1‑65 (MQ=255)
                                                                                                                                                                                                               tCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGc                                                                                                                                                                                                          <  1:140571/65‑1 (MQ=255)
                                                                                                                                                                                                               tCCG‑CAAACTGAAATCATGGCGC‑TTATCTCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAAc                             <  1:1097106/238‑1 (MQ=255)
                                                                                                                                                                                                               cccG‑CAACCTGAAATCTTTGCGC‑TTTTCTCCAAACCGCCGGCGCTTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAAc                             <  2:520704/237‑1 (MQ=255)
                                                                                                                                                                                                                ccggcaaagaaaaaTCTTTGCGT‑TTAGCCCCAACCCGCCGGCGCCGG‑GGGTGGCCCGCAAGGTGCTGGAAGGGGCGCGGGCCGGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAAc                             <  2:1516247/227‑1 (MQ=255)
                                                                                                                                                                                                                 cG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCa                           >  1:816999/1‑238 (MQ=255)
                                                                                                                                                                                                                       acgaaaaTCATTGCGC‑TTATCACAAAACCGCCTGCGCCTG‑CGGGGGCCCGCAAAGGGCTGGACCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGtt                     <  2:336088/235‑1 (MQ=255)
                                                                                                                                                                                                                       aCTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGtt                     <  1:1382176/239‑1 (MQ=255)
                                                                                                                                                                                                                         tGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGa                   >  1:1032605/1‑239 (MQ=255)
                                                                                                                                                                                                                          gAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGAt                  >  1:317497/1‑239 (MQ=255)
                                                                                                                                                                                                                          gAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGAGGCCCGCAAATTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTACTTCCTCGATCGTGGCGAAACGCCAGTGGATGGGCAGGGGCTACAGTTTGCCCGCGGCACCAACGAGGCAGCGCTACAAGAGGTGCTGCTCTCCGCCGTGAAAAAGCAAAATCTCCGCCTTCATCCGCTTAACCACCCGTTGAt                  >  2:954002/1‑239 (MQ=255)
                                                                                                                                                                                                                                   gcgc‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGATTGCGGAtgt         >  1:461044/1‑239 (MQ=255)
                                                                                                                                                                                                                                         tATCTCAAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGATTGCGGATgtgcgtg    <  1:1013303/239‑1 (MQ=255)
                                                                                                                                                                                                                                           tctcCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGATTGCGGATGtgcgtgcg  <  1:1247351/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
CGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGA‑CCTGAGCGAGAAAATCGGCGGCCT‑GATGATGCTCGACG‑CCATCGGGATGCTGGAAAACGATCCG‑CAAACTGAAATCATTGCGC‑TTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACCAGCCGTTGATTGCGGATGTGCGTGCG  >  NC_000913/337311‑337779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑