BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	502,887	T→A	9.7%	I118F (ATC→TTC)	ybaL ←	inner membrane putative NAD(P)‑binding transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,887	0	T	A	9.7%	394.1 / 3.9	148	I118F (ATC→TTC)	ybaL	inner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand): ref base T (47/83); new base A (14/2); total (63/85)
Fisher's exact test for biased strand distribution p-value = 1.90e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

TCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCT  >  NC_000913/502650‑503125

    tttGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCgg                                                                                                                                                                                                                                               <  1:1199634/239‑1 (MQ=255)

    tttGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACA‑CACGGTACTGGCGGGGGAAAGACATAAACAGAAAACGATACCgg                                                                                                                                                                                                                                               >  2:259403/1‑238 (MQ=255)

     ttGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt                                                                                                                                                                                                                                              <  1:1114790/239‑1 (MQ=255)

      tGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACAAGAAAACgggc                                                                                                                                                                                                                                             >  1:1460936/1‑237 (MQ=255)

              gTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACg                                                                                                                                                                                                                                      >  1:1461218/1‑238 (MQ=255)

                gATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAc                                                                                                                                                                                                                                    <  1:1140544/238‑1 (MQ=255)

                gATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAc                                                                                                                                                                                                                                    <  2:1586643/238‑1 (MQ=255)

                gATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAc                                                                                                                                                                                                                                    <  2:1631564/238‑1 (MQ=255)

                gATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAc                                                                                                                                                                                                                                    <  2:685048/238‑1 (MQ=255)

                  tCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAcca                                                                                                                                                                                                                                  >  1:519160/1‑238 (MQ=255)

                      cATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGCCATAAACCGCACACGAAAACGGTCATTTACCAccagccc                                                                                                                                                                                                                              >  2:756317/1‑238 (MQ=255)

                        tatCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccagc                                                                                                                                                                                                                           >  1:1188693/1‑239 (MQ=255)

                        tatCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAc                                                                                                                                                                                                                                    <  2:844750/230‑1 (MQ=255)

                        tatCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAAAGAc                                                                                                                                                                                                                                    >  1:844750/1‑230 (MQ=255)

                        tatCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGAACTAAACCCAACACGCAACCCGTCATTAAccgcccacccag                                                                                                                                                                                                                            >  2:167618/1‑227 (MQ=255)

                        tatCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGAAATAAACCGAACAAGATACCGGTCATTAACGAccagcccagc                                                                                                                                                                                                                           >  1:1047804/1‑239 (MQ=255)

                        tatCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCAGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAccagcccag                                                                                                                                                                                                                            >  1:1479536/1‑238 (MQ=255)

                             aCTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGTCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGAt                                                                                                                                                                                                                                                    >  1:206917/1‑209 (MQ=255)

                             aCTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGc                                                                                                                                                                                                                      >  2:1190478/1‑239 (MQ=255)

                             aCTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAAACGAACACGATACCGGTCATTAACGACCAGCCCAGCCCGGc                                                                                                                                                                                                                      >  1:665489/1‑239 (MQ=255)

                             aCTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAAACGAACACGAAACCGGGCAATAACGAccaccccacgccggc                                                                                                                                                                                                                      >  1:467597/1‑232 (MQ=255)

                                gCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCaga                                                                                                                                                                                                                   <  1:118101/239‑1 (MQ=255)

                                gCAAGAGTGGAAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCaga                                                                                                                                                                                                                   <  2:1140486/239‑1 (MQ=255)

                                      gtgtCAAAGCCCATACTGCCCTGTTCCACCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAAGCAACCAAGCGATGGCGATTTGCCTACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACGCCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAgcg                                                                                                                                                                                                              <  2:487848/238‑1 (MQ=255)

                                      gcagCAACGCCCCCATCGCCCTTTTCCATCCTCCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTGCCACAATCATCCAACCGACGGCGATTTGCCCACGCTGACTGTCAATTAAGTGCCGTTCTACAAGTGCGAGCATTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCGAGCACGGCAGAGAgcg                                                                                                                                                                                                              <  2:747892/235‑1 (MQ=255)

                                                        cccTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCg                                                                                                                                                                                            >  1:316482/1‑238 (MQ=255)

                                                         ccTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGc                                                                                                                                                                                           >  1:813516/1‑238 (MQ=255)

                                                           tGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCaga                                                                                                                                                                                                                   <  1:1227630/212‑1 (MQ=255)

                                                             ttCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGTAAGGCATAAACCGAACACGATACCGGGCATTAACCACCAGCCCAGCACGGCAGAGAGCGCCCTCCCCCGCAGCCTCGCCCCgg                                                                                                                                                                                      >  1:984576/1‑239 (MQ=255)

                                                              cccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATATCCAGCAGCGTCGCCACgg                                                                                                                                                                                      <  2:235762/237‑1 (MQ=255)

                                                                  tcatTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTa                                                                                                                                                                                   >  1:448575/1‑237 (MQ=255)

                                                                     ttCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGAc                                                                                                                                                                                                                                    <  1:621693/185‑1 (MQ=255)

                                                                       ccTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAAAACGATACCGGTCATTAACGACCAGCCCAGCACGGCGGAGAGCGCCATACCCACCAGCGTCGCCCCgccactgtgggc                                                                                                                                                                            >  2:1555622/1‑228 (MQ=255)

                                                                       ccTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAAACGAACACGATACCGGCCCTTAACAACCAGCCCAGCACGGCAGAGAGCGCCAAACCCAGCAGCGCCCCCACACCCATCCGGGc                                                                                                                                                                            >  2:615623/1‑239 (MQ=255)

                                                                               tGCGGGCAGCAACACCGGCATCAGAGCCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTGGCCCACGCTGACTGTCAATTAATTGCCGTGCTTCAAATGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCAc                                                                                                                                                                    <  2:1166980/239‑1 (MQ=255)

                                                                               tGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCAc                                                                                                                                                                    <  1:945864/239‑1 (MQ=255)

                                                                               tGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAAACGAACACGAAACCGGTCATTTACGACCAGCCCAGCACGGCAGAGAGGGCCATACCCAGCCGCGCCGCCCCCGCTGTCTGGGCGAACGCa                                                                                                                                                                     >  2:1573344/1‑238 (MQ=255)

                                                                                gCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCCGCCCAGCACGGCAGAGAGCGCCATACCCAACCGCGTCGCCACGGCTATCTGGGCGATCGCAcc                                                                                                                                                                   >  1:905750/1‑239 (MQ=255)

                                                                                gCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCAcc                                                                                                                                                                   <  2:1265327/239‑1 (MQ=255)

                                                                                gCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAAACGAACCCGATACCGGTCATTAACGACCAGCCCAGCACCGCAGAGAGCGCCCTACCCAGCAACGTCGCCCCGGCTATCTGGGCGATCGccc                                                                                                                                                                    >  2:317866/1‑236 (MQ=255)

                                                                                         aaCACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACA‑CACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑Cg                                                                                                                                                        <  1:259403/239‑1 (MQ=255)

                                                                                          acacCTGCGTCAGAACCATTACCAGGTCTTCCAGAATCAACCATCCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAGCCGAACACGATATCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCTCCGGGAATGT‑Cg                                                                                                                                                        <  2:81488/239‑1 (MQ=255)

                                                                                                  gTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGt                                                                                                                                                                                                                                              <  1:1661269/146‑1 (MQ=255)

                                                                                                       aaCCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑cc                                                                                                                                           >  1:498901/1‑238 (MQ=255)

                                                                                                          cATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACGTAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCa                                                                                                                                       <  1:1567942/239‑1 (MQ=255)

                                                                                                            ttACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGGACACGATACCGGTCATTTACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCCATCTGGGCGGTCGCCCCGGGAATGG‑CGGTGGCCTTTA‑ACGccct                                                                                                                                      >  2:550725/1‑236 (MQ=255)

                                                                                                                   gccctcaccaATCAACCGTCCCATGGCGATTTGCCCCCGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGAAACACCACGGTACTGGCGGTGGAAAGACATAAACCGACCACGATCCCGGTCATTAACGCCCAGCCCAGCACGGCGGAGAGCCCCATACCCAGCAGCGTCGCCCCGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑t                                                                                                                             <  2:1052616/231‑1 (MQ=255)

                                                                                                                   gTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCCATCTGGGCGATCGCACCGGGAATGG‑CGCTGGCCTTTA‑CCGCCATCAAATCC‑t                                                                                                                             >  1:268193/1‑239 (MQ=255)

                                                                                                                           caATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGaa                                                                                                                     <  1:1154164/239‑1 (MQ=255)

                                                                                                                           caATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGaa                                                                                                                     <  1:317866/239‑1 (MQ=255)

                                                                                                                           caATCAACCAACCGAGGGCGATTGGCCCACCCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACCCCACGGTACTGGCGGGGAAAAGACAGAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGaa                                                                                                                     <  2:33599/239‑1 (MQ=255)

                                                                                                                           caATCAACCAACCGAGGCCGATTTGCCCACGGTGACTGGCAATTAATTGCCGGTCCGCAAGTGCGGGCAGGAACACCACGGTCCTGGCGGTGGAAAGACAGAAACCTAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAAAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAAGGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGaa                                                                                                                     <  2:1030856/239‑1 (MQ=255)

                                                                                                                              tCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑Cg                                                                                                                                                        >  1:1507117/1‑203 (MQ=255)

                                                                                                                              tCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑Cg                                                                                                                                                        <  2:1507117/203‑1 (MQ=255)

                                                                                                                                aaccaaccGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAAACGAACACGACAACGGTCATTAACGACCAGCCCAGCAAGGCAGAGAGCGCCATAACCAGCAGCGTCGCCACGGCTATCTGGGCGGATGCACCCGGGATGG‑CGCTGGCCTTTA‑CCGCCACCAAAACC‑TTCAGCGAAAAga                                                                                                                 >  2:1411115/1‑237 (MQ=255)

                                                                                                                                     accGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAAc                                                                                                           <  1:1724577/239‑1 (MQ=255)

                                                                                                                                      ccGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAAACATAAACCGAACACCATAACGGTCCTTAACGACCCGCCCCCCACGGCCGAGAGCGCCCCAACCACCAACCTCGCCCCGGCCATTTGGGCGATCGCACCGGGAAAGG‑CGCAGGCCTTTA‑CCCCCCTCCAAACC‑TTCCGCCTAAAACGCAAAc                                                                                                           >  2:68355/1‑238 (MQ=255)

                                                                                                                                         aTGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGGAAGACATAAACCCAACACGCTACCCGGCCTTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCCCCACCGGCGCCCCCGCCCTCCGGGCGGTCGCACCGGGAATGGCCGATGGCCTTT‑‑CCCCCCCCCAATCC‑TTCAGCCTAAAGAGCCAACCGa                                                                                                        >  2:545443/1‑238 (MQ=255)

                                                                                                                                          tGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGCCATTAACGACCAGCCCAGACCGGCCGCGAGCGCCAAACCCAACAGCGTCGCCACGGGGATCTGGGCGGTCCCCCCGGGCAAGC‑AGATGGCCTTTA‑CACCCATCAAACAC‑CTAAGCGGAAAATGCAAACCggc                                                                                                       >  2:965817/1‑236 (MQ=255)

                                                                                                                                           ggCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAAccgccg                                                                                                      >  1:1679122/1‑238 (MQ=255)

                                                                                                                                           ggCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGc                                                                                                     <  1:167618/239‑1 (MQ=255)

                                                                                                                                            gCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGc                                                                                                     >  1:115970/1‑238 (MQ=255)

                                                                                                                                            gCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGAAACCGGTCCTTTACGACCAGCCCAGCACGGCAGAGAGCGCCCTAACCCGCCGCGGCGCCACGGCTATCCGGGCGATCGCACCCGGGATGG‑CCATGGCCCTTA‑CCCCCATCCAATCCTTTCCCCGAAAAG‑GCAAACCGgcgc                                                                                                     >  2:1655335/1‑238 (MQ=255)

                                                                                                                                              gATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCa                                                                                                   <  1:1411115/238‑1 (MQ=255)

                                                                                                                                               aTTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAAACATAAAACCAACACCATACCCGTCATTAACGACCAGCCCAGCACGGCAGAGAACGCCATACCCAGCACCGTCGCCACGGCTATCTGGGCGATCGCCCCCGGAAAGG‑CGATGGCCTTTA‑CCACCATCAAATCC‑TTCCCCGCAAAACGGAAACCGACGCCaa                                                                                                  >  2:629388/1‑238 (MQ=255)

                                                                                                                                                tttGCCCACGCTGACTGTCCATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACCGCCAGCCCAGCACGCCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAAc                                                                                                <  2:100005/239‑1 (MQ=255)

                                                                                                                                                  tGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACAt                                                                                              <  1:1491822/239‑1 (MQ=255)

                                                                                                                                                  ggCCCGCGCTGACTGTAAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGTTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATGAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACAt                                                                                              <  2:1679122/238‑1 (MQ=255)

                                                                                                                                                        cGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAg                                                                                        >  1:439202/1‑239 (MQ=255)

                                                                                                                                                            gACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGATCGCCATACCCAGCAGCGTCGCCACGGCTATCGGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATg                                                                                    <  2:1188693/239‑1 (MQ=255)

                                                                                                                                                                gTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGc                                                                                <  2:519160/239‑1 (MQ=255)

                                                                                                                                                                  cAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACCCCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGcc                                                                               <  1:615623/238‑1 (MQ=255)

                                                                                                                                                                  cAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAACCCAAACACGATCCCGTTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATCCCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGAAATGG‑CGATGGCCTTTACCCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGcc                                                                               <  1:508729/239‑1 (MQ=255)

                                                                                                                                                                     ttaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGTTCCCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGCCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGt                                                                           <  2:733374/239‑1 (MQ=255)

                                                                                                                                                                     ttaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑Cg                                                                                                                                                        <  1:1291123/164‑1 (MQ=255)

                                                                                                                                                                     ttaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGt                                                                           <  1:1361274/239‑1 (MQ=255)

                                                                                                                                                                     ttaattGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGt                                                                           <  1:1033395/239‑1 (MQ=255)

                                                                                                                                                                     ttaattGCCGTTCTTCAAGTGCGCCCAGAAACCCCACGGGACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGt                                                                           <  2:1316952/239‑1 (MQ=255)

                                                                                                                                                                           gCCGCTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCCAACACGATACCCGTCATTAACGACCAGCCCAGCACGGCAGAGAAGGCCATACCCAGCAGCGTCGCCACCGCCATCTCGGGGATCGCACCGGGAAAGG‑CGGAGGCCTCTT‑CCCCCCTCAAATCC‑TTCCACGGAAACAGAAAACCCACCCCAAACCTCACCCAAATGACGccccccttcgc                                                                      >  2:371858/1‑230 (MQ=255)

                                                                                                                                                                                tcttcAAGTGCGCGCAGTAACACCGCGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGtt                                                                 <  1:231150/238‑1 (MQ=255)

                                                                                                                                                                                     aaGTGCGCGCAGTAACCCCACGGTACTGGCGGTGGAAAGGCATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑gggg                                                          <  1:703792/239‑1 (MQ=255)

                                                                                                                                                                                          cgcgcAGTAACACCACGGTACTGGCGGTGGGAAGAAATAAACCGAACAAGAAACCGGGCCTTAACGACCAGGCCAGCACGGCAGAGAGGGCCATACCCCGCAGCGTCGCCAAGGCTATCCTGGCGATCGCACCGGGAATGG‑CGGTGGCCCTTA‑CCGCCAACCAATTC‑TTCAACGAAAAGGGCAAAACGACGCCAAAACCCAACAAAACTACACCCACGTCCGCCAGtttccgtgccag                                                       >  2:1504798/1‑228 (MQ=255)

                                                                                                                                                                                            cgcAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAAAACGATAACGGCCATTTAAGACCCAGCCAGCACGGCGTAGAGAACCATACCCCCCAGCGTCGCCACGGCAATCTTGGACCTCGCACCGGGGATGG‑GGACAGCCTTTC‑CCCCCACCAAAACC‑TTAAGCCAAAAGCGCAAAACGCCGCCAAACATCAAAAAAATAACACCCCGTTAAGCCAGTTCC‑GCGGCCAACtt                                                   >  2:650100/1‑239 (MQ=255)

                                                                                                                                                                                                        gcGGTACGGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGcaac                                       <  2:117143/238‑1 (MQ=255)

                                                                                                                                                                                                        acGGTACTGGCGGTGGAAAGACATAACCCGAACACGAAGCCGTTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGT‑CTATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGGCGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGcaac                                       <  1:1684671/239‑1 (MQ=255)

                                                                                                                                                                                                        acGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGcaac                                       <  2:439202/239‑1 (MQ=255)

                                                                                                                                                                                                         cGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGcaaca                                      <  1:1423771/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑Cg                                                                                                                                                        <  1:704785/124‑1 (MQ=255)

                                                                                                                                                                                                                ggCGGTGGAAAGACATAAACCGAACACGATCCCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCg                               <  1:646708/239‑1 (MQ=255)

                                                                                                                                                                                                                ggCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCg                               <  2:1479536/239‑1 (MQ=255)

                                                                                                                                                                                                                 gCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGc                                                                                >  1:606351/1‑190 (MQ=255)

                                                                                                                                                                                                                 gCGGTGGAAAGACATAAACCGAAAACGAGAACCGGCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGCCGCCACGGCCATCTGGGCGATCGCGCCGGGGAAGG‑GGAGAGCCTTTA‑CCCCCACCAAAACC‑TTCAGCGAAAAAGGCAAAACCCCGCCACACCTCAACAGAAAAAAGCCCCATTCAGCCAGATCCGGGGGCAACCTGGGGA‑CGGCAACAAAACCCgg                              >  2:1427442/1‑239 (MQ=255)

                                                                                                                                                                                                                      gtaaaGACATAAACAGAAACCGAAACCGAGCATTAACAACCACCCCAGAACGGCGGAGGGCCTCATACCCAGCAGCTTCGCCACGGCTTCCTGGCCGAACGCACCGGTAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAACTTGGTATCGGCAACAAAGCCCGGAGTGa                         <  2:234778/237‑1 (MQ=255)

                                                                                                                                                                                                                       ggaaGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCCATCTGGGCGATCGCACCCGGAAAGG‑CGATGGCCCTTa                                                                                                                                              >  2:704785/3‑124 (MQ=255)

                                                                                                                                                                                                                                     gAACCCGATACCGGTCATTAACGACCAGCCCAGCCCGGCAGAGAGCGCCTTACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGca          <  2:665489/239‑1 (MQ=255)

                                                                                                                                                                                                                                     gAACACGATCCCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGca          <  2:498901/239‑1 (MQ=255)

                                                                                                                                                                                                                                      aaCACGATACCGGCCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGtt                                                                          <  2:279196/175‑1 (MQ=255)

                                                                                                                                                                                                                                       acacGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGcaca        <  1:550725/239‑1 (MQ=255)

                                                                                                                                                                                                                                           gATACCGGTCATTAACAACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCCCGGCTATCAGGGCGATCGCACCGGGAATGT‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGCCGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGATATAGGAAACAAAGCCAGGAGCGACTGGTCATGCcaccccaccc     >  2:1472377/1‑238 (MQ=255)

                                                                                                                                                                                                                                             tACCGGTCATTAACGACCAGCCCAGCACGGCAGAGGGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCt  <  1:629388/239‑1 (MQ=255)

TCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGATACCGGTCATTAACGACCAGCCCAGCACGGCAGAGAGCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGG‑CGATGGCCTTTA‑CCGCCATCAAATCC‑TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCC‑GGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCT  >  NC_000913/502650‑503125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`