BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,435	C→T	5.0%	T94M (ACG→ATG)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,435	0	C	T	5.0%	459.6 / 11.4	139	T94M (ACG→ATG)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base C (63/69); new base T (5/2); total (68/71)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 139 total aligned reads displayed.

GAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGA  >  NC_000913/3927199‑3927669

gAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                             <  1:167925/239‑1 (MQ=255)

agtaactcctggctgtgtacgaagtggatctgatccggcaggaacggtgccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                             >  2:634065‑M1/220‑239 (MQ=255)

      tcgcTTTTCTCGTGAACCGGAAGAACGGCATGGGATGATCGAAGACCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGTATGCAGGATATCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑a                                                                                                                                                                                                                                      <  2:88314/236‑1 (MQ=255)

              ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCtt                                                                                                                                                                                                                             <  1:65358/239‑1 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCg                                                                                                                                                                                                                           <  1:858327/239‑1 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTc                                                                                                                                                                                                                            >  2:86646/1‑238 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCg                                                                                                                                                                                                                           <  1:15278/239‑1 (MQ=255)

                  tCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGc                                                                                                                                                                                                                          >  2:87720/1‑238 (MQ=255)

                   cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGcc                                                                                                                                                                                                                         <  2:1136560/238‑1 (MQ=255)

                   cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGcc                                                                                                                                                                                                                         <  1:1015019/238‑1 (MQ=255)

                              acggcgggggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGa                                                                                                                                                                                                              <  2:304656/231‑1 (MQ=255)

                               cGTCTGGGGCTGATCGAAGTCCATGCGCCGATGCTTAGCCTTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAGGCGGTTCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                           <  2:817562/239‑1 (MQ=255)

                               cGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCGCGCTGAAgtcg                                                                                                                                                                                                            >  1:480869/1‑236 (MQ=255)

                                 tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGGCC‑CCGTGAAGGCCg                                                                                                                                                                                                           >  2:941431/1‑237 (MQ=255)

                                 tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                           >  2:625057/1‑237 (MQ=255)

                                          gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGGCTTTCTCCGt                                                                                                                                                                                                >  2:397256/1‑239 (MQ=255)

                                           aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGtt                                                                                                                                                                                               >  2:265910/1‑239 (MQ=255)

                                           aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGCGCGAAGGGCTGTAAACCCACATG‑AA‑AGCCCTTCCCCC‑CGATGAAGACCGTCTTTTTCCGt                                                                                                                                                                                                >  2:944447/1‑238 (MQ=255)

                                              gAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCGTGATGCCCAGTGCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                            <  1:695915/239‑1 (MQ=255)

                                               aaGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGCTGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                            >  2:823920/1‑238 (MQ=255)

                                                aGTCCAGGCTCCGTTTTTTAGCCGTGTGGGGGATGGCTCGCAGTATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCCAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACCCGCTCATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                           <  2:809433/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                           <  1:931176/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                           >  1:836846/1‑238 (MQ=255)

                                                    cAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                      <  2:1127232/239‑1 (MQ=255)

                                                    cAGGCGCCGATTCTTAGCCGTGTGGCGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                      <  2:271932/239‑1 (MQ=255)

                                                          ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                                                                                                                >  2:1127999/1‑239 (MQ=255)

                                                           cGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCT‑TTCATGTGCGTGTAC‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGtt                                                                                                                                                                               <  1:357591/239‑1 (MQ=255)

                                                                cgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                          >  1:396370‑M1/156‑239 (MQ=255)

                                                                cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGGAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                          <  1:688990/239‑1 (MQ=255)

                                                                cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                          <  1:86646/239‑1 (MQ=255)

                                                                  tAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                                                                                                                                                                        <  2:329779/239‑1 (MQ=255)

                                                                      cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                                                                                                    <  1:941431/239‑1 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                           <  2:209742/214‑1 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTggg                                                                                                                                                                     >  2:1153624/1‑236 (MQ=255)

                                                                          tgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg                                                                                                                                                                >  1:463911/1‑239 (MQ=255)

                                                                            ggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                                                                               <  2:368755/238‑1 (MQ=255)

                                                                             ttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                             >  1:881‑M1/143‑239 (MQ=255)

                                                                              ggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                             <  1:1037982/238‑1 (MQ=255)

                                                                                    cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTa                                                                                                                                                       <  2:836846/238‑1 (MQ=255)

                                                                                       gtaatgcccgcccaaatcgcctctaccgatcgtttcagagtccagaaatgacgatcaccgaagcccatcacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATgg                                                                                                                                                   >  2:861785‑M1/133‑239 (MQ=255)

                                                                                         aGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                                                                                 >  1:1112793/1‑239 (MQ=255)

                                                                                             tAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTCCACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg                                                                                                                                             >  1:1223080/1‑239 (MQ=255)

                                                                                                   gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACCGTGAGc                                                                                                                                       >  2:66109/1‑239 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGACACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                      >  1:49310/1‑238 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                      >  2:746825/1‑238 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                      >  1:1162133/1‑238 (MQ=255)

                                                                                                           gtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACCCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAAt                                                                                                                                 >  2:54929/1‑237 (MQ=255)

                                                                                                             ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                              <  1:878830‑M1/80‑1 (MQ=255)

                                                                                                               aaaaGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTGCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCGCGa                                                                                                                           <  2:281608/239‑1 (MQ=255)

                                                                                                                 aaGCGGTGCAGGTAAAAGAGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAct                                                                                                                         <  1:834195/239‑1 (MQ=255)

                                                                                                                      ttgtaGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCCGTGGTACTGGGAACGCGTAATGGGCAGCGGTGAGCCTCAATTCTCGACCTTGaa                                                                                                                    >  2:138160/5‑239 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGCCTCTGaa                                                                                                                    >  1:742752/1‑239 (MQ=255)

                                                                                                                        gCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaaa                                                                                                                  >  1:1104071/1‑239 (MQ=255)

                                                                                                                          gtgTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                 <  1:1018317/236‑1 (MQ=255)

                                                                                                                          aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGTAACCCGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCG‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                 <  2:134377/238‑1 (MQ=255)

                                                                                                                          aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                 <  1:536298/238‑1 (MQ=255)

                                                                                                                             tAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCa                                                                                                               <  1:1153624/237‑1 (MQ=255)

                                                                                                                              aaacgggAAAGCTCTGCCTGATGTCCAGTTCGAAGTGGTTCATTGACTGCCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACGTCAGCGCTGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                            <  2:152057/233‑1 (MQ=255)

                                                                                                                              aaaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                            <  1:746825/239‑1 (MQ=255)

                                                                                                                                aaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTa                                                                                                          <  1:599574/239‑1 (MQ=255)

                                                                                                                                      aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                    >  1:657320/1‑239 (MQ=255)

                                                                                                                                               cTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGc                                                                                            <  2:1104071/238‑1 (MQ=255)

                                                                                                                                                          ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGc                                                                                >  1:1075721/1‑239 (MQ=255)

                                                                                                                                                           tCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaaa                                                                                  <  2:1035495/236‑1 (MQ=255)

                                                                                                                                                                    ttcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGaa                                                                         >  2:1062253/1‑236 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGACTTAAAGCAACCGAAGCTGCGGTTAga                                                             >  2:932924/1‑238 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGt                                                                 >  2:711271/1‑235 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGt                                                                 <  1:711271/235‑1 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                             >  1:106545/1‑239 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                             >  1:633031/1‑239 (MQ=255)

                                                                                                                                                                              ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCg                                                            <  1:87720/239‑1 (MQ=255)

                                                                                                                                                                                 gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGaa                                                          <  1:245590/238‑1 (MQ=255)

                                                                                                                                                                                     catagaccgagtgcaacggagcaagacggtcttcatcgGGGCGAAGGGCCGTACACGCACATG‑AA‑AGCCCTTCGACC‑CGAGAGAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGCCTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGGAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                     <  2:897147‑M1/201‑1 (MQ=255)

                                                                                                                                                                                      ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGTCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                     <  2:435096/238‑1 (MQ=255)

                                                                                                                                                                                      ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                     <  2:1075721/238‑1 (MQ=255)

                                                                                                                                                                                           ccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGcc                                                >  1:376550‑M1/33‑238 (MQ=255)

                                                                                                                                                                                              cAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                            <  1:423296/239‑1 (MQ=255)

                                                                                                                                                                                               aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                            >  1:201681/1‑238 (MQ=255)

                                                                                                                                                                                               aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACACGGTCTGTGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTGTCGACTCTGAAAAGCACGGTAGAGGCTATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                            >  2:962059/1‑238 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                      <  2:876056/237‑1 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGCCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCGGGCAcctgt                                      >  2:1173861/1‑234 (MQ=255)

                                                                                                                                                                                                          gaaCCCGACTTGAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAGCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGGGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                 <  2:784309/237‑1 (MQ=255)

                                                                                                                                                                                                          cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                 <  1:833056/238‑1 (MQ=255)

                                                                                                                                                                                                              acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                            <  1:1127999/239‑1 (MQ=255)

                                                                                                                                                                                                                  tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                        <  2:462654‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                  tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGAAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                        <  1:186300‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                   ttCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCGCTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGGCTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGAt                       <  2:513293/239‑1 (MQ=255)

                                                                                                                                                                                                                     ccagcgGGGCGAGGGGCGGTCCCCGAACATG‑AA‑CGCCCTTCGCCC‑CGATGAGGCCGGTCTTTCTCGGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGAAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCACCCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                     >  2:396370‑M1/7‑239 (MQ=255)

                                                                                                                                                                                                                     cAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                     <  1:1121666/239‑1 (MQ=255)

                                                                                                                                                                                                                         gcgGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACt                  <  1:625057/238‑1 (MQ=255)

                                                                                                                                                                                                                            GGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt              <  1:347976‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                               GAAGTGCTGTACACGCACATG‑AA‑AGCCCTTCGCAC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac            >  2:463911‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                               GAAGGGCTGTACACGCACATG‑AA‑GTCCCTGCGCCC‑CGATGAAGACCTTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac            >  2:881‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                     CTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc      >  2:800738‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                     CTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc      >  1:944447‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCa     >  1:405231‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg    <  1:211901‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTTACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGACAAGCACGGTCGAGGCGATCTGGGCGGGAATTACAGCAACCGAATCTGCGGTTAGCGAAGAGTTTAGCCTTGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg    <  2:741512‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                       gTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAACTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGAACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg   >  1:51509/1‑239 (MQ=255)

                                                                                                                                                                                                                                        tACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGCGAGCGTCAATTCTCGACTCTGAACAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGACGCTGCGGTTAGCGGAGGGTGTGGCCTGGCACCGTTCCTGCCGGACAAGAGCAACGTCGTACACAGCCAGGa  >  2:282910/1‑239 (MQ=255)

GAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGA  >  NC_000913/3927199‑3927669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`