BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,437	C→T	5.1%	H95Y (CAC→TAC) ‡	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,437	0	C	T	5.1%	438.1 / 12.5	138	H95Y (CAC→TAC) ‡	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base C (62/68); new base T (5/2); total (68/70)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

Only 100 of 141 total aligned reads displayed.

GAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTT  >  NC_000913/3927199‑3927672

gAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                               <  1:167925/239‑1 (MQ=255)

      tcgcTTTTCTCGTGAACCGGAAGAACGGCATGGGATGATCGAAGACCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGTATGCAGGATATCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑a                                                                                                                                                                                                                                        <  2:88314/236‑1 (MQ=255)

              ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCtt                                                                                                                                                                                                                               <  1:65358/239‑1 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCg                                                                                                                                                                                                                             <  1:858327/239‑1 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCg                                                                                                                                                                                                                             <  1:15278/239‑1 (MQ=255)

                  tCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGc                                                                                                                                                                                                                            >  2:87720/1‑238 (MQ=255)

                   cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGcc                                                                                                                                                                                                                           <  1:1015019/238‑1 (MQ=255)

                   cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGcc                                                                                                                                                                                                                           <  2:1136560/238‑1 (MQ=255)

                              acggcgggggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGa                                                                                                                                                                                                                 <  2:304656/231‑1 (MQ=255)

                              aCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGa                                                                                                                                                                                                                 <  2:572407/237‑1 (MQ=255)

                               cGTCTGGGGCTGATCGAAGTCCATGCGCCGATGCTTAGCCTTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAGGCGGTTCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCg                                                                                                                                                                                                              <  2:817562/239‑1 (MQ=255)

                                 tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGGCCCCGTGAAGGCCg                                                                                                                                                                                                              >  2:941431/1‑237 (MQ=255)

                                 tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCg                                                                                                                                                                                                              >  2:625057/1‑237 (MQ=255)

                                 tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCg                                                                                                                                                                                                              >  2:697316/1‑237 (MQ=255)

                                           aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGtt                                                                                                                                                                                                  >  2:265910/1‑239 (MQ=255)

                                           aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGCGCGAAGGGCTGTAAACCCACATG‑AA‑AGCCCTTCCCCCCGATGAAGACCGTCTTTTTCCGt                                                                                                                                                                                                   >  2:944447/1‑238 (MQ=255)

                                              gAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCGTGATGCCCAGTGCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                               <  1:695915/239‑1 (MQ=255)

                                               aaGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGCTGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                               >  2:823920/1‑238 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  1:1109524/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              >  1:836846/1‑238 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  1:377750/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  1:931176/238‑1 (MQ=255)

                                                    cAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                         <  2:1127232/239‑1 (MQ=255)

                                                    cAGGCGCCGATTCTGAGCCGTGGGGGGGATGGCACGCAGGATACCTTGCCGGGTTGTAAAAAAGCGGTGCAGGTAAAAGTGAAACCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATCCACGGGCGAAGTGAAAACGTCAGACCTTAGGGCAACACGCCTTCAGCGCGGGCGAAGGGCTGTCCACGCACCTG‑AA‑AGCCTTTCGCCCCAATAAAGCCCGTCTTTCTCCGTTGCACCCGGt                                                                                                                                                                                         <  2:80508/239‑1 (MQ=255)

                                                          ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                                                                                                                   >  2:1127999/1‑239 (MQ=255)

                                                          ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGCAGTGGAAACGTCAGACCTTAGGTCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AC‑AGCCCTTCGCCCCGCTGAAGACCGTCTTTCTCCGTTGCACTAGGCCTCTGt                                                                                                                                                                                   >  2:889627/1‑239 (MQ=255)

                                                           cGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCT‑TTCATGTGCGTGTAC‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGtt                                                                                                                                                                                  <  1:357591/239‑1 (MQ=255)

                                                              ttccctaaccgcagcttcggttgcttgaattcccgccgcgaccgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactgggcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCCCGATGAGGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAc                                                                                                                                                                               >  2:376550‑M1/158‑239 (MQ=255)

                                                                cgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                             >  1:396370‑M1/156‑239 (MQ=255)

                                                                cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                             <  1:86646/239‑1 (MQ=255)

                                                                  tAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                                                                                                                                                                           <  2:329779/239‑1 (MQ=255)

                                                                      cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                                                                                                       <  1:941431/239‑1 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              >  1:209742/1‑214 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  2:209742/214‑1 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTggg                                                                                                                                                                        >  2:1153624/1‑236 (MQ=255)

                                                                         gtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGGGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTAAACGCACATG‑AC‑ACCCCTTCTCCCCGCGGAAGACCGTGTTTCTCCGTGGCACTCGGCCTATGTTGCCCTGCGGGACta                                                                                                                                                                    >  2:400491/1‑238 (MQ=255)

                                                                          tgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg                                                                                                                                                                   >  1:463911/1‑239 (MQ=255)

                                                                             ttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                                >  1:881‑M1/143‑239 (MQ=255)

                                                                              ggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                                <  1:1037982/238‑1 (MQ=255)

                                                                                aTGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAAcgc                                                                                                                                                             >  1:435096/1‑239 (MQ=255)

                                                                                    cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTa                                                                                                                                                          <  2:836846/238‑1 (MQ=255)

                                                                                       gtaatgcccgcccaaatcgcctctaccgatcgtttcagagtccagaaatgacgatcaccgaagcccatcacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTGCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATgg                                                                                                                                                      >  2:861785‑M1/133‑239 (MQ=255)

                                                                                         aGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                                                                                    >  1:1112793/1‑239 (MQ=255)

                                                                                             tAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTCCACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg                                                                                                                                                >  1:1223080/1‑239 (MQ=255)

                                                                                                   gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACCGTGAGc                                                                                                                                          >  2:66109/1‑239 (MQ=255)

                                                                                                     cGGGCTGTGCCAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGTAGTGGTTCATTCACTGGCGCAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGCAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTTTCCGTTGCACTCGGTCTATGTTGGCCACTGGGACTGGGAACGCGTTATCGGCGCCGGTGAGCg                                                                                                                                         >  2:864781/1‑238 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGACACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                         >  1:49310/1‑238 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                         >  2:746825/1‑238 (MQ=255)

                                                                                                             ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                                 <  1:878830‑M1/80‑1 (MQ=255)

                                                                                                               aaaaGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTGCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCGCGa                                                                                                                              <  2:281608/239‑1 (MQ=255)

                                                                                                                 aaGCGGTGCAGGTAAAAGAGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAct                                                                                                                            <  1:834195/239‑1 (MQ=255)

                                                                                                                      ttgtaGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCCGTGGTACTGGGAACGCGTAATGGGCAGCGGTGAGCCTCAATTCTCGACCTTGaa                                                                                                                       >  2:138160/5‑239 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                 >  2:485312/1‑229 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                 <  1:485312/229‑1 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGCCTCTGaa                                                                                                                       >  1:742752/1‑239 (MQ=255)

                                                                                                                          gtgTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                    <  1:1018317/236‑1 (MQ=255)

                                                                                                                          aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGTAACCCGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCGCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                    <  2:134377/238‑1 (MQ=255)

                                                                                                                          aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                    <  1:536298/238‑1 (MQ=255)

                                                                                                                             tAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCa                                                                                                                  <  1:1153624/237‑1 (MQ=255)

                                                                                                                              aaaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                               <  1:746825/239‑1 (MQ=255)

                                                                                                                                      aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                       >  1:800738/1‑239 (MQ=255)

                                                                                                                                      aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                       >  1:657320/1‑239 (MQ=255)

                                                                                                                                               cTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGc                                                                                               <  2:1104071/238‑1 (MQ=255)

                                                                                                                                                          ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGc                                                                                   >  1:1075721/1‑239 (MQ=255)

                                                                                                                                                           tCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaaa                                                                                     <  2:1035495/236‑1 (MQ=255)

                                                                                                                                                             gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAAc                                                                                <  1:675118/239‑1 (MQ=255)

                                                                                                                                                                    ttcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGaa                                                                            >  2:1062253/1‑236 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGACTTAAAGCAACCGAAGCTGCGGTTAga                                                                >  2:932924/1‑238 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                >  1:106545/1‑239 (MQ=255)

                                                                                                                                                                              ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCg                                                               <  1:87720/239‑1 (MQ=255)

                                                                                                                                                                                 gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGaa                                                             <  1:245590/238‑1 (MQ=255)

                                                                                                                                                                                      ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGTCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                        <  2:435096/238‑1 (MQ=255)

                                                                                                                                                                                      ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                        <  2:1075721/238‑1 (MQ=255)

                                                                                                                                                                                              cAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                               <  1:423296/239‑1 (MQ=255)

                                                                                                                                                                                               aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACACGGTCTGTGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTGTCGACTCTGAAAAGCACGGTAGAGGCTATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                               >  2:962059/1‑238 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                         <  2:876056/237‑1 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGCCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCGGGCAcctgt                                         >  2:1173861/1‑234 (MQ=255)

                                                                                                                                                                                                          gaaCCCGACTTGAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTGCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAGCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGGGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                    <  2:784309/237‑1 (MQ=255)

                                                                                                                                                                                                          cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                    <  2:181175/238‑1 (MQ=255)

                                                                                                                                                                                                             cacGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGa                                >  2:275264/1‑239 (MQ=255)

                                                                                                                                                                                                              acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                               <  1:1127999/239‑1 (MQ=255)

                                                                                                                                                                                                              acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                               <  2:302987/239‑1 (MQ=255)

                                                                                                                                                                                                                gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCa                             <  1:697316/239‑1 (MQ=255)

                                                                                                                                                                                                                  tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                           <  2:462654‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                  tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGAAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                           <  1:186300‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                   ttCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCGCTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGGCTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGAt                          <  2:513293/239‑1 (MQ=255)

                                                                                                                                                                                                                     ccagcgGGGCGAGGGGCGGTCCCCGAACATG‑AA‑CGCCCTTCGCCCCGATGAGGCCGGTCTTTCTCGGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGAAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCACCCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                        >  2:396370‑M1/7‑239 (MQ=255)

                                                                                                                                                                                                                     cAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                        <  1:1121666/239‑1 (MQ=255)

                                                                                                                                                                                                                         gcgGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACt                     <  1:625057/238‑1 (MQ=255)

                                                                                                                                                                                                                         gcgGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACt                     <  1:59310/238‑1 (MQ=255)

                                                                                                                                                                                                                            GGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt                 <  1:347976‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                               GAAGGGCTGTACACGCACATG‑AA‑GTCCCTGCGCCCCGATGAAGACCTTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac               >  2:881‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                               GAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac               >  1:1201662‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                     CTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc         >  2:800738‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                     CTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc         >  1:944447‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg       <  1:211901‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTTACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGACAAGCACGGTCGAGGCGATCTGGGCGGGAATTACAGCAACCGAATCTGCGGTTAGCGAAGAGTTTAGCCTTGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg       <  2:741512‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                       gTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg      >  1:629654/1‑239 (MQ=255)

                                                                                                                                                                                                                                       gTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAACTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGAACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg      >  1:51509/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt  >  2:575696/1‑237 (MQ=255)

GAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTT  >  NC_000913/3927199‑3927672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`