Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,195,008 | C→G | 5.5% | intergenic (‑143/+91) | sibD ← / ← sibE | sRNA antisense regulator of toxic IbsD protein/sRNA antisense regulator of toxic IbsE protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,195,008 | 0 | C | G | 5.5% | 210.5 / 4.1 | 63 | intergenic (‑143/+91) | sibD/sibE | sRNA antisense regulator of toxic IbsD protein/sRNA antisense regulator of toxic IbsE protein |
Reads supporting (aligned to +/- strand): ref base C (29/30); new base G (2/2); total (31/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 |
GGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCA > NC_000913/3194721‑3195286 | ggAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc > 2:300852/1‑288 (MQ=255) agaagGGGGCCTTACAGAAGGAAAGGGTGATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCaaaa < 1:472956/289‑1 (MQ=255) tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGa < 1:354217/289‑1 (MQ=255) gatgtaaGGAAAAGGTTATGATGAAGCTGTTCATCATACTGATTGTGTTGTTATGCGTAAGTTTGGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTC‑‑‑‑‑CTTTAGGTT‑TAAAAAAAAGCCGCGCCACGAAGGTTTCATTATCCCCTTCCCCGGGACCGTCAAG‑CGTGTGTATGTGACAGTCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCa < 2:272859/284‑1 (MQ=255) aaGGAAAGGGTTATGATGAAGCTAGTAATCATACTGATTGGGTTAGTACTCGGAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAAGCCTCCATCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGGCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCa < 1:31945/289‑1 (MQ=255) ttATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCgggg > 1:448683/1‑289 (MQ=255) aaGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTCTTGTTTCTTATAAACATC‑TCACCCTGAAGACTACGTGAATACATAACACTTGCCCAGTAAGCGGGGGATTATATAAA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAACTTGCCTCCGGGAAAGCAGTGCGCGGGGTCCGgcg > 2:214528/1‑288 (MQ=255) aGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGgcgc < 1:421546/288‑1 (MQ=255) aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCGGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCgggg‑g > 1:129815/1‑288 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg > 2:350026/1‑224 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg < 1:350026/224‑1 (MQ=255) gttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑Ggcagca < 2:448683/289‑1 (MQ=255) tACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAggg > 1:466288/1‑288 (MQ=255) cGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGa < 1:401428/289‑1 (MQ=255) ttCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACggg < 1:300852/288‑1 (MQ=255) attaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTc > 2:428773/1‑288 (MQ=255) gCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCtc > 1:516456/1‑235 (MQ=255) gCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCtc < 2:516456/235‑1 (MQ=255) ggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑gggggccttacag > 2:522900/1‑280 (MQ=14) gagaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCTAAAACGCCGGGGACTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAAGGCTCAGGACGTTTCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCATCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGCA‑GCGGCCTTGgatacg > 2:103726/1‑287 (MQ=255) actctgccctcACGCTTGTTACTTTGATTTAGGTT‑GCAAAAAAATCAGCGTCCAAAGGGTTTCATTAACACATAGCGCTGGCCCAAAAAGCCCGGGATTTTTTATA‑TCTTCGTTCCGCTAAAAGGTGCAAATGCTCAGAACGTCGCAGCCTTTTGCGTGACCGGCTGGGGAAGGGAAAAGGGCCTAGGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGaaa < 2:174480/283‑1 (MQ=21) aaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGaaa > 1:44293/1‑289 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAgg > 1:415555/1‑289 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGta < 2:71851/278‑1 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGta > 1:71851/1‑278 (MQ=255) tGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCt < 1:428773/289‑1 (MQ=255) gTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTc > 1:317870/1‑289 (MQ=255) gTTCCTCTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGA‑AGc > 2:141514/1‑288 (MQ=255) ttCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCg < 2:317870/289‑1 (MQ=255) ttactttaGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGTCTGAAACGGGAAAGCCCCTACCGAAGCA‑GGGGCCTTGTATTAGGAAAGGGTTATGATCA‑AGCTCGtact > 2:77882/1‑285 (MQ=255) ttaGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATAc < 1:141514/289‑1 (MQ=255) tttCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc < 1:239205/288‑1 (MQ=255) ttCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt > 2:522928/1‑289 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGcc > 2:9901/1‑201 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGcc < 1:9901/201‑1 (MQ=255) tGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTACAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCCCGGGGCAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCCCCCCAAGAA‑GGGGCCTTGAATCAGGAAAGGGTTATGCTGA‑ATCTCGTCCTCATACTGGTTGTGTTTTTACTTTTAAGTTTCCCGACTTCCTCACCACTCATCagac > 2:414953/1‑288 (MQ=18) ggCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAgg > 1:484019/1‑289 (MQ=255) aaaaGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:282707/1‑272 (MQ=255) aaaaGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa < 2:282707/272‑1 (MQ=255) aaaaGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGgag > 1:91349/1‑289 (MQ=255) aaaaGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGgag > 2:532249/1‑289 (MQ=255) aaaGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGgaga > 1:337685/1‑289 (MQ=255) cGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAAtc < 1:532249/288‑1 (MQ=255) cGGGGATTTTTTATA‑TCGGCGTTCCGCTAAAAGGTGCAAAGGCTCAGGCCGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTTAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAAtc < 2:466288/288‑1 (MQ=255) ta‑tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTg > 2:258536/1‑289 (MQ=255) ta‑tCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTg < 1:214528/289‑1 (MQ=255) gCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCt < 2:91349/289‑1 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCtt > 1:482119/1‑289 (MQ=255) cGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGATGAAACGGGAAAGCCACTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGATTTACTAACAACTCATCAGAGGGGGGAGAAATCGCCCCATACCCTTGTTcc > 2:385786/1‑287 (MQ=255) cGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGcc < 1:457060/161‑1 (MQ=255) cGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGcc > 2:457060/1‑161 (MQ=255) tGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGa < 1:522928/287‑1 (MQ=255) tGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGcc > 1:471965/1‑133 (MQ=255) tGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGcc < 2:471965/133‑1 (MQ=255) cAGCGTTTTGCGTGAACGCTCGGGATAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGTGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 1:55737/289‑1 (MQ=255) cgggTTTGGCGGGACGGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCAGTGCGCGGGGTGCGGCGCTCATCAAAAAGCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 2:374095/285‑1 (MQ=255) agggtgtagagTGACCGCGGGGGGGAGGGAAAAGTGGCCATTGGAAAGAATTGCAGGGGGTCCGGCGGTCCTCAAGTATCGGGG‑GGTGTCAAGGGGGTGAAACGGCAAAGGCCCTTACACAGAC‑GGGGTCTTGGGTAGCGAAAGGGGTGT‑ATAAGAGATAGTCATCATACTGATTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGTGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 2:129815/278‑1 (MQ=255) cGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt > 2:70041/1‑288 (MQ=255) tttGCGTGGCCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCa < 1:103726/289‑1 (MQ=255) tttGCGTGACCGCGCGGGGAAGGCAAAATTGCCTCTGTGAAAGCAGTGACCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCGGAAACGGGAAGGCCCCTCCGGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGcc < 2:314151/288‑1 (MQ=255) gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGa > 1:127250/1‑129 (MQ=255) gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGa < 2:127250/129‑1 (MQ=255) gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAacaaca < 2:477688/264‑1 (MQ=255) gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAacaaca > 1:477688/1‑264 (MQ=255) | GGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTT‑GAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATA‑TCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGG‑GGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAA‑GGGGCCTTGTATAAGGAAAGGGTTATGATGA‑AGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCA > NC_000913/3194721‑3195286 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |