| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 828,729 | T→C | 6.2% | M85V (ATG→GTG) | ybhF ← | putative ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 828,729 | 0 | T | C | 6.2% | 210.3 / 3.2 | 65 | M85V (ATG→GTG) | ybhF | putative ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (26/35); new base C (2/2); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
TCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCA > NC_000913/828444‑829011 | tCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCAtata > 1:216202/1‑289 (MQ=255)tCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCCCCATCACCTTTAGATTCTCATACAGACCAAATTTCTGCGGCAtata > 2:168943/1‑289 (MQ=255) ggcagcggTTCGCGCCGTGGGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACCCCTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAGCGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAAc < 2:223322/282‑1 (MQ=255) cgcgCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGc > 1:168689/1‑289 (MQ=255) gggTCAACGCCGACGCCGGGTTCTACGAGCAGAAACGCTTTCGGTTCGCCCTCCAGGGTACAGGCCAAACCGCGTGTTTGTTTAATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGCGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCGTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGATGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCcg < 2:529360/288‑1 (MQ=255) aaCGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGttt > 1:34512/1‑289 (MQ=255) cgccgacgccgGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGttttt > 2:491841/1‑289 (MQ=255) gacgccgGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATc < 1:183657/289‑1 (MQ=255) gacgccgGGTTCATCGAGCAGCAACACGTTCGGTGCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACGCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATc < 1:110019/289‑1 (MQ=255) acgccgGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCACATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCg > 1:389309/1‑289 (MQ=255) gggTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCa < 2:34512/289‑1 (MQ=255) aTCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCACATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa > 1:259131/1‑289 (MQ=255) cGAGCAGCAACACTTTCGGTTCGCCCACCAGTGTACAGGCCATACCGAGTGTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAAt < 1:260404/289‑1 (MQ=255) tttCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTg < 1:12402/289‑1 (MQ=255) cGGTCCGCCCACCAGGGTAGAGGCCAGACCTGGTTGTTGTTTCAGTCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGTCCCAAGAGACGTATACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCg < 2:374583/289‑1 (MQ=255) cccACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCgg > 1:53038/1‑289 (MQ=255) ggTACAGGCCATACCGAGTTTTGGTTTCACCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACACCAGCAGGCGAGCAAAAGTTTGCTTACGGGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATGGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAg < 2:191929/288‑1 (MQ=255) aCAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATCGAGGTTCTCCATCACCGCGAGATCTTCATACAGCCCAAATTTCTGCGGCATATAACCGAGAACGGCGTGCACCGCTCCGTCGTTTTTGATCCGATCAACGCCACTCACCGTGCACCTGCCGCTGTCTGGtttacgtac > 2:28398/1‑282 (MQ=255) gCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCCTATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCCCCGTTGCCTTGCCGCTGTCGGGTTTTAGTACTccc > 2:208581/1‑288 (MQ=255) gCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATccc > 2:280402/1‑288 (MQ=255) aCCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt < 2:355904/231‑1 (MQ=255) aCCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGAt > 1:355904/1‑231 (MQ=255) aCCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa < 2:305947/244‑1 (MQ=255) aCCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCa > 1:305947/1‑244 (MQ=255) aCCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCaa > 2:92623/1‑288 (MQ=255) aCCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAAc > 1:431782/1‑289 (MQ=255) gTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATcc > 2:442864/1‑288 (MQ=255) ttCATCCCACCGGGGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCACATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCg < 2:389309/289‑1 (MQ=255) ttCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGc < 1:423601/288‑1 (MQ=255) tCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 1:491841/289‑1 (MQ=255) tCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 1:195946/289‑1 (MQ=255) cccccccGGAGGGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGGGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 2:168689/288‑1 (MQ=255) ccccACCGGGGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 2:274137/288‑1 (MQ=255) accaaCCGGAGGGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTGTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 2:516976/285‑1 (MQ=255) aacgaCCGGCGACCTCGCCAGCCGGGCGTCCGGAAAAGCGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAGGTTTGCTTACGTCCCGCGCCGGTGACCCTCCGCAAATCCGCGTACCGATTGAGGATCTGCATCACCGTGAGATCGTCATACAGACCAAATTTCGGCGGCATATAACCGAGCGCGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCTTGGt < 2:329428/285‑1 (MQ=255) agCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 1:170288/278‑1 (MQ=255) agCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt > 2:170288/1‑278 (MQ=255) cGCCAGGGGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCGGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTGCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGACCGGc < 2:361279/289‑1 (MQ=255) gTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCaa < 1:517409/289‑1 (MQ=255) ggTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAAcccc < 1:47097/289‑1 (MQ=255) aaCGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACTTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTca < 2:501565/289‑1 (MQ=255) cGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTcaca < 1:280402/289‑1 (MQ=255) ggCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCCCTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCCGCGTGGGTTTACCTGCCCCTGCCGGCCCCCACAACCCCGTcacca > 2:229957/1‑287 (MQ=255) cccAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATa > 1:17246/1‑288 (MQ=255) ccaaaggcaCGTAAACTCCAGCAGGCGGGCAAAAGTTTGCTTACGGGCCTCGCCGGTGACGCTCCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGCGATCTTCATACAGACCAAATTTCTGCGGCACATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATaa < 2:259131/281‑1 (MQ=255) gaCGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGc < 2:53038/287‑1 (MQ=255) gaCGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGc < 1:442864/287‑1 (MQ=255) cGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTg < 1:168943/288‑1 (MQ=255) gTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGGGaa > 1:278737/1‑289 (MQ=255) aaaaGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAgcggcg < 1:92623/287‑1 (MQ=255) cTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGc < 2:431782/289‑1 (MQ=255) cTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGCAAGCGtt > 1:199914/1‑289 (MQ=255) tCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAgg > 2:344626/1‑288 (MQ=255) ggTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATaa < 1:208581/288‑1 (MQ=255) tcCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATTACGGca > 1:174528/1‑288 (MQ=255) ttCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCGCGGACGTTCCGCGTGATAACGGCATCATTCATTTCCGGCCt > 2:499408/1‑288 (MQ=255) gACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGtt < 2:369345/247‑1 (MQ=255) gACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGtt > 1:369345/1‑247 (MQ=255) aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAg < 1:208507/195‑1 (MQ=255) aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAg > 2:208507/1‑195 (MQ=255) aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCGCTTCAGCGGGATAACGGCATCCTTCATGCCCTCCCTCGGCCCCGaa > 2:349631/1‑289 (MQ=255) aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACTGCATCATTCATGTCCTGCCTCGTCACCGaa > 1:97846/1‑289 (MQ=255) aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGaa > 1:25122/1‑289 (MQ=255) aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGaa > 1:31623/1‑289 (MQ=255) ccAAAATGCTGCGGCATATAACCGGGCAAGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGaa < 2:199914/288‑1 (MQ=255) aTTGCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTa < 1:109837/289‑1 (MQ=255) cgggctataACCGGGCACGGCTTGCAGCGCGCCGCCGTTTTTGCTCGAATCAAGGCCAATCACCTTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCa < 2:216202/283‑1 (MQ=255) cGGCATATAGCCGAGCACGGCGTGCGGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATGCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCa < 1:28398/288‑1 (MQ=255) | TCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCCGGAAAGCGTTTTTCCAGGCCGTTCAGCGTGATAACGGCATCATTCATGTCCTGCCTCGTCACCGAATTGTACCGTCA > NC_000913/828444‑829011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |