Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 75 [0] [0] 78 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)67 (1.160) 55/268 0.0 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

TGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  >  NC_000913/257766‑258048
                                                                                                                                             |                                                                                                                                             
tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                              <  1:1018313‑M1/143‑2 (MQ=255)
  ttAAAAATTTGCAAAATTGTGCCAATTGGGTAAAACAGTTGCGGCACAACAGGAGATAGCAATGACGGGACGGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                            <  2:166017‑M1/143‑4 (MQ=255)
    aagaatttGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGTAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgc                                                                                                                                          >  2:1067676‑M1/4‑138 (MQ=255)
    aaaaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCGGTGGATTCGTGAAGGTAagtgc                                                                                                                                          >  1:200451‑M1/1‑138 (MQ=255)
        aTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                      >  1:371782‑M1/1‑134 (MQ=255)
            gCTAAATTTTGCCAATTTAGTAAAACAGTTGCATCACAACAGGAGATAGCAAAGACGTTACCGAGTGGACACCCGAAGAGCAGATGGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataa                                                                                                                                  <  1:360411‑M1/143‑14 (MQ=255)
             cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                 <  1:642791‑M1/143‑15 (MQ=255)
                aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                              <  2:646330‑M1/143‑18 (MQ=255)
                 actttgcCCATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgat                                                                                                                             <  1:824734‑M1/141‑19 (MQ=255)
                 aTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGCGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgct                                                                                                                             >  2:466719‑M1/1‑125 (MQ=255)
                  ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGCAGGGAagtgaaaagataatcgctt                                                                                                                            >  2:323798‑M1/1‑124 (MQ=255)
                        ccattTGGTATAACAGTCGCATCACAACAGGAGATAGCAATGACGATACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                      <  1:1032243‑M1/141‑26 (MQ=255)
                        cAATTTGGTAAAACAGTTGCATCACAACAGGTTGTAGCAATAACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                      <  1:489469‑M1/143‑26 (MQ=255)
                         aaTTTGTTAAAAAAGTTGCTTAACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAATATTTACCGCACTAGGCCCGTATATTCGTTAAGGTAagtgcaaagataatcgattctttttc                                                                                                                     <  2:982153‑M1/143‑27 (MQ=255)
                         aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                     >  1:906291‑M1/1‑117 (MQ=255)
                         aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                     >  1:896163‑M1/1‑117 (MQ=255)
                           tttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgaatctttttcga                                                                                                                   >  1:11567‑M1/1‑115 (MQ=255)
                             ctgtaAAACAGGCGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATGTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                 <  1:412529‑M1/141‑31 (MQ=255)
                                tAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGGATATTCGTGAAGGGAagtgcaaagatagtcgattctatttcgattgtc                                                                                                              >  2:738511‑M1/1‑110 (MQ=255)
                                 aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtct                                                                                                             >  2:874369‑M1/1‑109 (MQ=255)
                                 aaaaCAGTGACATCACAACAGAAGATATCAATGACGTTACCGAGTCGACACCCGAAGAGCATATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtct                                                                                                             <  2:184070‑M1/143‑35 (MQ=255)
                                   aaCATTTCCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                           <  2:773598‑M1/143‑37 (MQ=255)
                                           tataacaacaagagATAGCAAAGACGTAACCGGGGGGACACCCGAAGAGCAGATTGACCAAAAAATTTACCGCACGAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                                                                   <  1:452454‑M1/139‑45 (MQ=255)
                                               gcaacagGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgcctgtatgcgtca                                                                                               >  2:156132‑M1/2‑95 (MQ=255)
                                                caacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaa                                                                                              <  2:615803‑M1/143‑50 (MQ=255)
                                                 aacaGGAGATTGTTATGTCGTTATCGAGTGGACAACCGAAGAGCAGAAGGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                             <  1:38436‑M1/143‑51 (MQ=255)
                                                   caGGATATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                           <  1:881142‑M1/143‑53 (MQ=255)
                                                   caGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                           <  1:912477‑M1/143‑53 (MQ=255)
                                                         aTAGGAATGACGTTATCGAGTTGACACCCGAAGAGCAGATTGATCAAATTTTTTATCGCACTTGGCTCGTATATGCGTGAAGGGAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                     <  1:301001‑M1/143‑59 (MQ=255)
                                                              aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGGATATTCGGGAAGGTAagtgccaagataatcgattctttttcgattgtctggctgtctgcgtccccttgacaccagcac                                                                                >  1:905094‑M1/1‑80 (MQ=255)
                                                                       atcgaGCGGACACCCGGAAAGCCGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                       <  2:428035‑M1/141‑73 (MQ=255)
                                                                         ctattggacCCCCGACGTGCAGAGTGATCAAAAAATTTACCGCACTAGGCCCGCATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaacccggcaccggaagtgcgt                                                                     <  2:181181‑M1/139‑75 (MQ=255)
                                                                         cGAGTGGACACCCGAGGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                     <  1:979630‑M1/143‑75 (MQ=255)
                                                                         cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCTCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttgttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                     <  2:1010756‑M1/143‑75 (MQ=255)
                                                                               gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCTTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                                                               <  2:506921‑M1/143‑81 (MQ=255)
                                                                                 cacCTGAAGCTCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                             <  2:2035‑M1/143‑83 (MQ=255)
                                                                                 cacCCGAAGAGCAGCTTGACCAAAAACTTTACCTCTTTGTGCCCGTATATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                             <  1:36098‑M1/143‑83 (MQ=255)
                                                                                  acCCGAAGAGCATATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcttcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                            <  1:274949‑M1/143‑84 (MQ=255)
                                                                                  acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                            >  1:733366‑M1/1‑60 (MQ=255)
                                                                                  acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                            <  2:335786‑M1/143‑84 (MQ=255)
                                                                                   cgcgacgagcTGGTTGCTCAGAGAAGTTGCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctgtgtcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggg                                                           <  2:535795‑M1/141‑85 (MQ=255)
                                                                                     cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggcagtgcgtgaattctggggc                                                         >  1:300779‑M1/1‑57 (MQ=255)
                                                                                      gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgagttctcggggt                                                        >  2:30884‑M1/1‑56 (MQ=255)
                                                                                       aaGAGCAGATTGATCAAAACATTTCCCTCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctg                                                       <  2:1020981‑M1/143‑89 (MQ=255)
                                                                                       aaGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgggaacggaagtgcgtgaattctggggctg                                                       >  2:99590‑M1/1‑55 (MQ=255)
                                                                                            cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                  >  1:308115‑M1/1‑50 (MQ=255)
                                                                                             aGATTGATCAAAAGATGTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaggataagcgattcgtttgcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                 <  2:187073‑M1/143‑95 (MQ=255)
                                                                                              gcttgaTCCAAAAATGTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                <  1:1040701‑M1/141‑96 (MQ=255)
                                                                                                 tGATCAAGAAATTTACCGCACTAGTCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                             <  2:807187‑M1/143‑99 (MQ=255)
                                                                                                 tGATCAAAACGTTTACCGCACTAGGCCCGTATACTCGTGAAGGTAagtgcaaagataagcggttcttttgcgattgtctggctgtatgcgtcaacgtgaaaccggccccggaagtgcgtgaattctggggctggtggatggag                                             <  1:133286‑M1/143‑99 (MQ=255)
                                                                                                   aagaaaaaatttACCGCACGAGGCCCGTATATTCGTGAAAGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                           <  2:774855‑M1/140‑101 (MQ=255)
                                                                                                   aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtagacgtgaaaccggcaccggaagtgggtgagttctcgcgctggtggatggagac                                           >  2:590930‑M1/1‑43 (MQ=255)
                                                                                                      aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggctggcgctgga                                        >  1:788436‑M1/1‑40 (MQ=255)
                                                                                                      aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                        >  1:9774‑M1/1‑40 (MQ=255)
                                                                                                       aacagtttatcgcGATAGGCCCGGATATTCGTGAAGGTAagtgcaaagataatcgattcgtttgcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                       <  1:879742‑M1/138‑105 (MQ=255)
                                                                                                        aaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                      <  1:955282‑M1/143‑106 (MQ=255)
                                                                                                            tttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgagaacgtgaaaccggcactggaagtgcgtgaattctggggctggtggatggaggtggaggggca                                  >  2:496108‑M1/1‑34 (MQ=255)
                                                                                                             ttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                 <  1:379913‑M1/143‑111 (MQ=255)
                                                                                                                ccGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaa                              >  1:241485‑M1/1‑30 (MQ=255)
                                                                                                                ccGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaa                              >  1:960041‑M1/1‑30 (MQ=255)
                                                                                                                ccGCACTAGGCCCGGATATTCGTGAAGGGAagtgcaaagataatcgattctttttcgattgtcgggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaa                              <  1:737655‑M1/143‑114 (MQ=255)
                                                                                                                      tAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgt                        <  2:308115‑M1/143‑120 (MQ=255)
                                                                                                                         gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttt                     <  1:570727‑M1/143‑123 (MQ=255)
                                                                                                                           ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgggtcaacgtgaacccggcaccggcagtgcgtgaattctggggccggtgaactgaactggacgcaaaggaatcacgatttac                   >  1:582080‑M1/1‑19 (MQ=255)
                                                                                                                               atatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaatgtgagttctgaaccagaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctac               <  1:156132‑M1/143‑129 (MQ=255)
                                                                                                                                tatTAGTGAAGGGAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca              <  1:822158‑M1/143‑130 (MQ=255)
                                                                                                                                 atTCGTGACGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgggagcgcaggaatcccgttttacctagag             >  1:473816‑M1/1‑13 (MQ=255)
                                                                                                                                   tcttgaaGGTAagtgcaaagataatcgattctttttcgattggctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagtt           <  1:374920‑M1/140‑133 (MQ=255)
                                                                                                                                      tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgcaaccggcaccggaagtgcgtgaattctggggctggtggctggagccggaagcgcaggaatccggttttacctcgagttcac        >  2:176232‑M1/1‑8 (MQ=255)
                                                                                                                                      tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctggatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacc        >  1:1066334‑M1/1‑8 (MQ=255)
                                                                                                                                       gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctggatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca       <  2:632469‑M1/143‑137 (MQ=255)
                                                                                                                                           gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggaactggaagcgcaggaatcccgttttacctacagtgcccagttc   >  2:1011631‑M1/1‑3 (MQ=255)
                                                                                                                                           gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtaaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccaggtt   >  2:1003717‑M1/1‑3 (MQ=255)
                                                                                                                                            tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg  <  2:200451‑M1/143‑142 (MQ=255)
                                                                                                                                            tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg  <  1:766583‑M1/143‑142 (MQ=255)
                                                                                                                                             |                                                                                                                                             
TGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  >  NC_000913/257766‑258048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: