Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,466 A→G 15.0% M2V (ATG→GTG)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4660AG15.0% 151.4 / 8.5 73M2V (ATG→GTG) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (13/48);  new base G (8/3);  total (22/51)
Fisher's exact test for biased strand distribution p-value = 1.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCG  >  NC_000913/3206236‑3206703
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 
tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGGTGAGCGa                                                                                                                                                                                                                                           <  1:681629/238‑1 (MQ=255)
tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                           <  1:1212992/238‑1 (MQ=255)
       cAGCTATTTAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCATTGTATATCGCAAAACAAGGCCATTCCCAGCCCTGACGCAATATTCATTCTTATTGTCTCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATATTTAGTGGTAGTTTGTAGCGGGGAATTTATCCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCGAATGtaat                                                                                                                                                                                                                                    <  2:475181/238‑1 (MQ=255)
       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                    <  2:322679/238‑1 (MQ=255)
         gCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACCAAAAAAAAAAATAGGGTTGCCCTTATGAGCGAAAGgaataaa                                                                                                                                                                                                                                 >  1:117852/1‑238 (MQ=255)
               cAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAATGGGTTGTCCTGATGTGCGCAAAtaaaaaaaaaaaa                                                                                                                                                                                                                           >  1:353338/1‑232 (MQ=255)
                  cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAAAAAAAATGGAGTTGTCATGTTGAGGGAAAGtaaaaaaaaaaagaca                                                                                                                                                                                                                        >  1:609553/1‑229 (MQ=255)
                   aTCGTTAATTATCCGCATTTGTGATAAGCGCAGTGTATTTCGCAAAACATCGCCATTTCCGTCTCTGGCGTAATATTCATTCTTATTTTAGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACATAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                         <  2:418246/237‑1 (MQ=255)
                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                          <  2:679385/236‑1 (MQ=255)
                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                          <  1:466107/236‑1 (MQ=255)
                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                          <  1:245308/236‑1 (MQ=255)
                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                         <  1:527421/237‑1 (MQ=255)
                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                         <  1:405568/237‑1 (MQ=255)
                   aTCGTTAATTATCCGAAGTTGCGATAAGCGCAGTGTATTTCGCAAAACATTGTCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                          <  2:884321/236‑1 (MQ=255)
                        tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCTATATTCAGTAGTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTGCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                   <  2:362577/238‑1 (MQ=255)
                           tgctCCGCAGTTCGGATACGCGCAGTGTATTTCTCAAAACATTGCCATTACCTGCTTTAGCGCAATATTCATTCTTATGTTCATGTTGATTTACGAACTCTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGGTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGTCACTGAATAAg                                                                                                                                                                                                               <  2:165387/236‑1 (MQ=255)
                                cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTATAAAAATAAAAAAAATGGAGGTGTCATGGTGAGGGGTAGTAATAAACAACAAGCAATGAACAAGTGGAc                                                                                                                                                                                                          >  2:42500/1‑239 (MQ=255)
                                 gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATATTTTCAGACAAATACAAAAAATGGGGTTTCCAGGGTTGGCGAAAGATAAAAGCAAAACGCAGTGAATAAATTGGCa                                                                                                                                                                                                         >  1:766827/1‑239 (MQ=255)
                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                     <  1:849748/238‑1 (MQ=255)
                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                     <  2:560479/238‑1 (MQ=255)
                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                    <  1:74784/239‑1 (MQ=255)
                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                    <  1:457775/239‑1 (MQ=255)
                                          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACACAAAAACAAAAAATTGAGTTGCCATGATGAGAGGAAATAAAAAAAAAAAAGAAATGAATAAGTTTAAAAAAATTGTc                                                                                                                                                                                                >  2:757226/1‑239 (MQ=255)
                                          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTATACAAAAAAAAAAAATGACGTTGTCCTTAGCAGGGCAAAGTATAAAAAAAAAGCAATGAAAAAGTGTACAGAGATTg                                                                                                                                                                                                  >  2:13286/1‑237 (MQ=255)
                                             aGCTCAGTTTATTTTGAAAAACATTGCCATTCCCTGCTCTGTCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAATTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACAATTTTAGTGGTATTTTGTGGCGGTAAATTTTTCAAAACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                             <  2:609553/239‑1 (MQ=255)
                                             aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                             <  1:13286/239‑1 (MQ=255)
                                                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCAGCAAAAACAAAAAATTGAAATTTCCAGATTGGGGGAAGTAATAAAGAACAAGCAAGGAAAAAATTGACAAAGAATTTTGCTAACattt                                                                                                                                                                                      >  1:1045251/1‑238 (MQ=255)
                                                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAATTTTTAAAAAAAAAACAAAAAATGTGATTTGCCAGAGATGAGAGAGGTAAAATACATAAGAAGATAATAAGCTGTCAGAGATTCTTGCTAACttt                                                                                                                                                                                       >  2:1124421/1‑238 (MQ=255)
                                                          tcccAAAACTTCGCTATTCCCGGCTTTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATTTCCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTATTTTGGGGGGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCACCAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                                                                                                                                               <  1:909580/236‑1 (MQ=255)
                                                            acAAAACATTGCCATTCCCTGCTCTGGCGAAATATTAATTCTTATTTTCTCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGATTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTACTTTTACCGCCATg                                                                                                                                                                             <  1:391680/238‑1 (MQ=255)
                                                              aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAATAAAAATTGAGTTTTGCTTATTAGCGTAAATAATAAAAAATACGAAGAGAATAAATTAGAACAGAGTGTCGCTCACCTTATACCCATGAt                                                                                                                                                                           >  1:854016/1‑239 (MQ=255)
                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAGACAAAACAAAAAATACAGTTGCCATCATGGGCGGAAGGAATAAGACACAAGCAGTGACAAAGTTGACAAAGATTATTGATAACTTTtcctcccttcttt                                                                                                                                                                         >  2:915883/1‑232 (MQ=255)
                                                                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGTAGGTGCCATGATTAGCGAAAGTAATAAAAAACAGGGAGGGAATAAGTTTACAGAGAGTGTGGCTAAATTTACCCCCATGATTTCTAc                                                                                                                                                                    >  1:1101461/1‑238 (MQ=255)
                                                                        taTTCCCTTGTTTGCCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAATGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTCCCAAACAAATAAAAAAACTGGAGTCGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                <  1:847011/238‑1 (MQ=255)
                                                                         aTTCCCTTCTCGGGCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                               <  1:829109/239‑1 (MQ=255)
                                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                         <  1:1021953/180‑1 (MQ=255)
                                                                                  aaaaagcaaaTATTCATTCTAATTTCGGCGTTAATTCCAAACTTGTTAATTACAATTTGAGGGTGGAAAAAAATAAAAAACAAAATTTTAAGTGGTAGTTGGTGGGGGTGAATTTTTCAAAAAAAAAAAAAAAACGGGAGTGGCCATGAGGAGCGAAAGTAATAAGCAACAGGTAGTGAATAAGTTGACGGAGATTGTCGTTACCTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                                                      <  2:339379/231‑1 (MQ=255)
                                                                                            tataCATTCTTATTTTCGCGTTAATTCACGACCTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTACCAGAAAAATACAAAAACTGGAGTTGCAATGAGGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                            <  1:166732/239‑1 (MQ=255)
                                                                                            aattcactcTTATTTTCGGTTTGATACACAAAGTGTTAATTACATATGCACGGTGGAAAAAAATAAAAAACAAAATTTTGAGGGGTAGTTTGTGGGGTAGAATTTTTCCAGAAAAAAAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAATAAATAAGTTGACAGATATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                            <  2:125645/238‑1 (MQ=255)
                                                                                                    cTTATTTTCGTGTTTATTCACGAACTGTAAATTAAATATTCACGGTGAGAAAAAAAATAAAACCAAATTTTGAGGGGGAGTTTGTGGCGGTGAATTTTTCAAGAAAAAAACAAAAACTGGTGTTGCCATGATGAGCGAAATTAATAAGCAACAGGGAGTGAATAAGTTGAAAGAGATTGTCGATAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                    <  1:608541/239‑1 (MQ=255)
                                                                                                    cTTATTTACGGATTGATCCATGATCTGTTAATTAAATATTAAGGGTGAAAAAAAATAAAAAACCACATTTTAAGTGGAATTTAGTGGCGGTGAATTTTACCAGAAAAAAAAAAAACCGGGATTTGCAATGATGAGCGAAAGTAATAACCAACAGGTAGTTAAAAAGTGGAAAGAGATTGTCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                    <  2:529126/239‑1 (MQ=255)
                                                                                                    cTTATTTACGCTTTAATTCACGAATTGTTAATTACATATGCACGGTGGCAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATAATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                    <  1:148751/239‑1 (MQ=255)
                                                                                                     ttATTTTCGTGTTAATCCACAAACTGTTAATTACATTTTCACGGTGGCAAAAAATAAAAAACAACATTTATAGGGGTGGTTTGTGGCGGTAAATTTTCCAAAAAAAATACAAAAACGGGAGTTCCAATAAGGATCAAAATAAATAAGCAACAGGCATTAAATAAGTGGAAAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                   <  2:161297/239‑1 (MQ=255)
                                                                                                      tATTTTCGCGTTGATTCACGAACTTTTAATTACATATCCCCGGGGGAAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                  <  1:42500/239‑1 (MQ=255)
                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTATACAAAAACAAAAACTTGAGTTGCCATTGTGTGGGTAACGAAAAAAAAAAAAGCACAGAAAAAATTTGAAAAGATTTTGCATAACTTTTACGCCCATATTTCTACCCGAATGCCTCGTGAAGCGGTGGGTAAACTaaa                                                                                                                                   >  2:702996/1‑238 (MQ=255)
                                                                                                      tATTTTCGCGTTGATTCAAGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                  <  1:154220/239‑1 (MQ=255)
                                                                                                                               gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAAAGGAGTTTGCATTGTGGGGGAAAGGAAAAAAAAAAAAGGAAGGAAAAAATTGACAGAGATTTTCGCTAACCTTAACCCCAAGATTTTTTACCGAATGACTGGAGACGGGGGGGGTAAAAAAAAAAAACTTAAAGAACCCGaaaaggcc                                                                                                          >  2:25389/1‑234 (MQ=255)
                                                                                                                                   attaattatTCAGGGGGAAAAAAAATATAAAACAACATTTATAGTGGTAGTTTGTGGGGGTAAATTTTACCAGACAAATACAAAAACGGAAGTTGCCATGATGAGGAAAAGTAATAAGCAACAGGCAGTTAATAAGTTGACTGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa                                                                                                     <  1:344051/233‑1 (MQ=255)
                                                                                                                                    ttAAATATCCACGTTGCAAAAAATAATAAAACCACTGTATTAGTGGTAGTTTGTGTCGGTGAATTATGCCAGAAAAATACAAAAACGGGAGTTTCAAGGATGAGCAAAATTAATAAGCAACAGGAAGGTAATAGGTTGACAGAGATTGTCGATATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                                                                                    <  2:322415/239‑1 (MQ=255)
                                                                                                                                         tgttcggggTGGCAAAAAATAAAAAACCAAATTGTTAGTGGTAGTTTGTGGGGGTGAATTTTCCCAAAAAAAAACAAAAACTGGTGTTGCCAGAATGAGGAAAATTAAAAAGCAACGGGCAGTGAATAGGTGGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                               <  2:1045251/232‑1 (MQ=255)
                                                                                                                                         tatTCAGGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAATTTTTTCCAGACAATTAAAAAAACAGGAGTGGCCATGAGGAGGAAAAGTAAAAAGAAACAGGCGGGGAAGAAATGTAAATAGGTGGCCGGAAGCTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                               <  1:109395/239‑1 (MQ=255)
                                                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGAGTTTCCATGATGAGCGGAAAGAATAAACAAAAAGCAGTGAAAAAGATTACAGAGATTGTCGCTACATTTACCCCCATGATTTCTACCCGAAAGCCTGATGACGTGGTGGAGAAACTAAAAAAACTAAAAGAAGCCGGAACATCGTCGATggggga                                                                                              >  1:958632/1‑237 (MQ=255)
                                                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAATTGAGTTGCCATGATGAGCGCAAGTAATAAACAACAAGCAGGGGAAAAAATGACAGAGAGTTTCGCTAAATTTACCGCCATGATTTTTACCCGAATACCTGATGAAGGGGGGGGTAAACTAAAAAAACTAAAGGATGCCGAAAACACGTCGGTGGGGaa                                                                                              >  1:1101168/1‑239 (MQ=255)
                                                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCTGACAAATAAAAAAAATGGGGGTGTCATTGAGGGCGGAAGGTAAAAACAAAAAGAAAAGAATAAATTTATAGAGAACGTCGCTAACTTTACACCCCAGATTTTTTACCCAAATGCTGAGGCAGGGGTGGGTAAAATAAAAAAAATTAAAGATATCCAAAAGTCGTCGAtatggga                                                                                              >  2:726296/1‑233 (MQ=255)
                                                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGAGTTGGCATGGTGAGCGAAAGTAATAAACAAAAAGCAGAGAAAAAAATGATAGAGATTGGCTGTAAATTTACCACCACGATTTTTACCCGAAGACCAGATGACGCGGGGGATAAACAAAAAAAAACAAAAAATTCCGACAcgtcgtacaggggggg                                                                                              >  2:966271/1‑229 (MQ=255)
                                                                                                                                              aCGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                          <  1:757226/239‑1 (MQ=255)
                                                                                                                                              aCGGTGGAAAAAAATAAAAACCCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAAAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                          <  1:470818/239‑1 (MQ=255)
                                                                                                                                                ggtgaaaaaaaaTAAAAAACCACATTTTTAGTGGTAGTTGGGGGCGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                        <  1:25389/233‑1 (MQ=255)
                                                                                                                                                 ggggCAAAAAATATAAAACCATATTTTTGGGGGTAGGTTGGGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGAGGAGGGAAAGTAATACGCAAAGGGTAGTGAATAGGTTGACAGAGATTGTCGTTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                        <  1:164518/236‑1 (MQ=255)
                                                                                                                                                  tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGAGGTTGCCTTGGTGGGGGAAATAAAAAAAAAACAGCAAAGAATAAGGTGAACAAGATTTTCCCTTAATTTAACACCAATATTTTTAACCCGATGCCCTGTTACGGTGTGGGAAAAACAAAAAAAGTCAAAGAAACCCAAAACGCCGGCGTGGGGGAAATTAttt                                                                                      >  2:285026/1‑237 (MQ=255)
                                                                                                                                                        aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                <  1:1124421/239‑1 (MQ=255)
                                                                                                                                                          aaTATAAAACCACATTTTTGGTGGTAGTTGGGGGGGGTGAATTTTTCAAAAAAAATACAAAAACTGGAGTTGCCATGAGGAGCAAAAGTAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                              <  1:915883/239‑1 (MQ=255)
                                                                                                                                                                 aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAAATGAGGTGGCATGATGGGGGAAAGGAATAAAAAACAAGCAAAAAAAAAAATGAAAGAGAATGTCGCCAACTTTACACCCATGATTTCTACCCCAAAAACTGATGACGGGGGGGATAAACTAAAAAAGTCAAACAGAGCCGCAACATCGTCGGTGGGGGAAATTATATTCCCCACGAAGTTc                                                                        >  2:350200/1‑239 (MQ=255)
                                                                                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTTGAGGTTTCATGGTGCGACATAATAAAATACAAAAGACATTGAATGAGGTGTAAGAATTTCTAGCTTAAATTACCGCCCTCAGTTTTACCACCACGACTGATGACGTGGTGGATATACTCACACACCTTAATGCGGCCACAACATCGCTCGAGGGGGAAATTATAATCAATACCATGTTCTacaaa                                                                  >  2:12608/1‑238 (MQ=255)
                                                                                                                                                                                 ggTAGTTTGGGGGGGGAAATTTTTCAAAAAAAAACAAAAACTGGGATTTGCAATGATGAGGAAAAGTAAAAAGAAACAGAAAGTGAATAGGTTAAAAGAGATTGCCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                          <  2:83476/237‑1 (MQ=255)
                                                                                                                                                                                     gtgtgggggggTAAATTTTGCAAAAAAAAAACAAAAAGTGAAGTTGCCAGGGTGAGCGAAAGTAATAAGCAAGAGGAAGTAAATAAGTGGAAGGAAATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                     <  2:353338/229‑1 (MQ=255)
                                                                                                                                                                                                        ttcccAGACAAATAAAAAAACTGGAGTTGCCATGGTGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                   <  1:538631/236‑1 (MQ=255)
                                                                                                                                                                                                                  aaTAGAAAAACAGAAGTTGCAATGAGGAGCGAAATTAATAAGCAACAGGAAGTGAATAAGTAGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                         <  1:884732/239‑1 (MQ=255)
                                                                                                                                                                                                                     aCAAAAACGGGATTTGCCATGATGAGCGAAATTAATAACCACCAGGAAGTAAATAATTTGACAGAGATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGacac                      <  1:1054575/239‑1 (MQ=255)
                                                                                                                                                                                                                          aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                  >  2:55895/1‑239 (MQ=255)
                                                                                                                                                                                                                          aaaCTGGAGTGGCGATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAAAAAGTGAACAGAGATTGACGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                  <  2:401234/239‑1 (MQ=255)
                                                                                                                                                                                                                           aaCTGGAGTTGCCATGATGAGCGAAAGTAATAACCAACAGACAGTGAATAAGTTGACAGGTATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                 <  1:915853/239‑1 (MQ=255)
                                                                                                                                                                                                                           aaCTGGAGTTGCCATGATGAGCGAAAATAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCCCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                 <  1:382661/239‑1 (MQ=255)
                                                                                                                                                                                                                                              gatgaGCGAAAGTAATAAGCAACAGGCAGTGAATACGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACTTGAATCGTCCTGCCTGTCAGGACACCGGAGAGATTATGTTCTTCg  >  2:643429/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 
TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCG  >  NC_000913/3206236‑3206703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: