Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,576,077 C→A 15.0% D515Y (GAT→TAT)  yddB ← putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,576,0770CA15.0% 184.7 / 3.2 61D515Y (GAT→TAT) yddBputative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (45/5);  new base A (2/8);  total (48/13)
Fisher's exact test for biased strand distribution p-value = 1.89e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTAT  >  NC_000913/1575847‑1576313
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGATTGCTTTTGATAATTTGATCATGCGATTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGTTTTTCTGCTGCTGTCCCATTGCCAGTTCATCGTTATCAGGCGTTTTCAAATCCTGATCACGCGTCAGAGTTTTCTTACCTGTTACCGATTCCGTCCAGCTATTACGGATATCACGTTATCCCATTTCacgcatct                                                                                                                                                                                                                                      >  2:372496/1‑232 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  1:110312/1‑239 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  1:55195/1‑239 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  2:563173/1‑239 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCAAGCTATTGCGGATCTCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  2:1065844/1‑239 (MQ=255)
  tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATAtt                                                                                                                                                                                                                                     >  2:592613/1‑238 (MQ=255)
  tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATAtt                                                                                                                                                                                                                                     >  1:124897/1‑238 (MQ=255)
  tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATAAAGAATAtt                                                                                                                                                                                                                                     >  1:949385/1‑238 (MQ=255)
     tggtggTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTccc                                                                                                                                                                                                                                  >  1:553358/1‑238 (MQ=255)
     tggtggTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTccc                                                                                                                                                                                                                                  >  1:337557/1‑238 (MQ=255)
       gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGcc                                                                                                                                                                                                                               >  1:651016/1‑239 (MQ=255)
          gtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATa                                                                                                                                                                                                                            >  1:1090785/1‑239 (MQ=255)
           ttttAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAg                                                                                                                                                                                                                           >  1:407447/1‑239 (MQ=255)
            tttAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCTGCCATAGt                                                                                                                                                                                                                          >  1:846968/1‑239 (MQ=255)
                    cgcTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTta                                                                                                                                                                                                                  >  1:273506/1‑239 (MQ=255)
                        gTCGGTACGATTTCTTTTGCTGTTTTGATCATGCGCTTCACGGTAAAAATAGTTTGCACGCGCAATAACTTTCTTACCGATTTTCTGCTGCAATCCCATTGCCATTTCATCTTTATAAGGCTTTTTAAAATCCTGATAACGCGTCAGATTTTTTTTACCTAATACCTATCCCGTCCAGCTATTGCGGATATAACTAAACCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAAc                                                                                                                                                                                                              <  1:563173/239‑1 (MQ=255)
                                   tGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATc                                                                                                                                                                                                   >  1:216931/1‑239 (MQ=255)
                                   tGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCACATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAAta                                                                                                                                                                                                   >  2:1090410/1‑238 (MQ=255)
                                                    tCATGCGCTTCACGTTAAACATAGTTTGCACGCGAAATAACGTTCTTACCGATTTCTTGCTGCAACCCCATTGCCTTTTCATCTTTAAAAGGCTTTTTAAAATCCTGATACCGCGTCATATTTTTATTACCTAATACCGATTCCGTCCAGCTATTGCGAATATCACGTACCCCAATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAg                                                                                                                                                                                   <  1:592613/238‑1 (MQ=255)
                                                        gcgcTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                                                                                                                                                              >  1:372333/1‑239 (MQ=255)
                                                          gcTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAat                                                                                                                                                                            >  1:1078909/1‑239 (MQ=255)
                                                                     aaCATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCg                                                                                                                                                                 >  1:449967/1‑239 (MQ=255)
                                                                        aTAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCAGATGCAATCCCATTGCCAGTTCACCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCACAGTTTTATTACCAGATACCGATTCCGTCCAGCTATTGCGGATATCAAGTACTCCCATATTAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATAACTGTAGCTTGATTATCAACAATATCCCAATCCgc                                                                                                                                                                >  1:993454/1‑236 (MQ=255)
                                                                             ttGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaaa                                                                                                                                                          >  1:1190509/1‑238 (MQ=255)
                                                                             ttGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTCATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaaa                                                                                                                                                          >  2:1159125/1‑238 (MQ=255)
                                                                             aatagcaagcAATACCTTTCTCACCTATTTTCTTCTGCACCCCCATTGCCACTTCTTCTTTATAAGGCTTTTTAAATCCTTGAAAGCGTTACAGTTTTTTTTTACCTAACCCCTATCTGCCCCAGCTATGGCGGATTTATCTAAACCCTATATCAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAg                                                                                                                                                         <  2:831976/231‑1 (MQ=255)
                                                                                     gaaaTAACTTTCTTACGGATTTTCTCCTCCACCCCAATTGCCATTCAATGTTAAAAAGGCTTTTCAAAATCTGAAAAACGCGTCAGATTTTTATTCCCGGATACCGATCCCGCCCACTTTTTGCGAATACCACTTAACCCAATACAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgggg                                                                                                                                                  <  1:12770/236‑1 (MQ=255)
                                                                                            tttttgtACCGTTTTTCCGCTCAACTCCCATGGCCATTTATTCGTTATAGGTCTTTTACAAACCCATATAACGCGTAAGATTTTTATTCCTTGATCCCGTTTCCGCCCAGCTTATGGGAATATCACGTAACCCAATATCAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATtg                                                                                                                                          <  2:337557/233‑1 (MQ=255)
                                                                                            ctttcttcCCGTTTTTCTGCTACAACCCCCTTCCATTTTCATGTTTATAGGTCGTTTTCAAATCTTTATACCGCGTCATTTTTTTATTCCTTGATACCGACTCTGTCCAGTTATTGCGATTATCACATAATCCCATATAAAGATTTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATtg                                                                                                                                          <  2:449967/237‑1 (MQ=255)
                                                                                                cTTGCCGATTTTCTGCGCCAAGCGCATGGCCAGTTATTTGTTAGAGGTCGTGTTGAAATGTTTAAAGTGCGTCATATTTTATTTACCTGATCGGGTTCCATCCCAGTTATTGGAGCTACAAGGAAATCCCAAATAAAAATTTTGCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGt                                                                                                                                      <  2:369642/239‑1 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAACGCGCGGGGAGATATTGTGGtt                                                                                                                                     >  1:169729/1‑239 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCTCGGGGAGATATTGTGGtt                                                                                                                                     >  1:786878/1‑239 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGgaga                                                                                                                                               >  1:831976/1‑229 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATAATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGTTATTGTGGtt                                                                                                                                     >  1:159314/1‑239 (MQ=255)
                                                                                                   aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTg                                                                                                                                   >  1:1111707/1‑239 (MQ=255)
                                                                                                   aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTg                                                                                                                                   >  1:311156/1‑239 (MQ=255)
                                                                                                   aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGATTTCATATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATAAAGAATATTCCCGACATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTAGATTAGCAAAAATAGCACTTTACTACATAAATCGTGGGGAGAGTTTGTGTTTTg                                                                                                                                   >  2:255576/1‑239 (MQ=255)
                                                                                                           tCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAg                                                                                                                           >  1:923805/1‑239 (MQ=255)
                                                                                                               ctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTg                                                                                                                        >  1:1071677/1‑238 (MQ=255)
                                                                                                                                 ttCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                      >  1:902772/1‑238 (MQ=255)
                                                                                                                                  tCATCGTTAAAAGGTGTTTCAAAATCCTAAAAACCGGTCAGTTTTTTATTCCCTAATCCCGATTCCGCCCAGTTTTGGGAGATATCACAAAACCAAATAAAAAAATTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGcc                                                                                                    <  2:110312/239‑1 (MQ=255)
                                                                                                                                           taAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTaa                                                                                           >  1:617061/1‑239 (MQ=255)
                                                                                                                                              ggCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGATATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGCTGTCATAGCCATACCGCCCGCCGCGCATTAATg                                                                                         >  2:105307/1‑238 (MQ=255)
                                                                                                                                                cGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGac                                                                                       >  1:863532/1‑238 (MQ=255)
                                                                                                                                                  ttttCAAATCCTGATAACGAGTCAGAGTTTTATTACGTGACACCGATACCGTCCAGCTATTGAGCATATCACGTACTCCCATATCAACAATATTCAGACGATAGTAACGGTTATAACTCGCTGTACTCACAGCAGCTTGATTAGCAAACATATCCAATTCCGTACTATAGAGCGGGCCGTTATCGTGGTTTGCCAGATCAGTATCATAGTCATACCGCACGCCGGGCATTACTggaaca                                                                                    >  2:223426/1‑234 (MQ=255)
                                                                                                                                                         aataccgaaTAACGGGTCATTTTTTTTTACCCTAATACCGCTTCCTACCAGCTATTGCGAATAACACAAACCCCAATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACg                                                                               <  2:273506/231‑1 (MQ=255)
                                                                                                                                                                     cgcgTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaaa                                                                                                                                                          >  1:475058/1‑152 (MQ=255)
                                                                                                                                                                        gTCAGATTTTATTTGCCTAATACAAATTCCCTACATTTATTGCTAATATAACGTACCCCAAAATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGAt                                                                 <  1:372496/238‑1 (MQ=255)
                                                                                                                                                                                           aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                             >  1:945255/1‑239 (MQ=255)
                                                                                                                                                                                           aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                             >  1:425660/1‑239 (MQ=255)
                                                                                                                                                                                                 aTTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATaa                                                                                                                                                                                                               >  1:839383/1‑71 (MQ=255)
                                                                                                                                                                                                  ttCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGGAACGGTTATAACCTGCTGTAATCATTGCGGTTTGACTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGAGGTTTGACAGATAGTTGTCATAGGCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGAGATCAGCCATATACGGCGTGTAGTTGCCaa                                      >  1:23643/1‑239 (MQ=255)
                                                                                                                                                                                                      gTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTc                                   >  1:721783/1‑238 (MQ=255)
                                                                                                                                                                                                       tCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTccc                                 >  1:520554/1‑239 (MQ=255)
                                                                                                                                                                                                           acttatgtggaatatCACGTACTCATAAAAAAAAAATATCACCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAgg                              <  2:520554/228‑1 (MQ=255)
                                                                                                                                                                                                             cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcg                            >  1:522880/1‑239 (MQ=255)
                                                                                                                                                                                                                  gCGGATATCCCGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGGTATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCCGGCGGcatt                       >  2:371610/1‑239 (MQ=255)
                                                                                                                                                                                                                      atatCACAAAACCCCATAACAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                  <  2:171429/239‑1 (MQ=255)
                                                                                                                                                                                                                          cACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTAt              >  1:171429/1‑239 (MQ=255)
                                                                                                                                                                                                                                acagtaatacAATAATCTTGCCGCCAGAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaa         <  2:617061/230‑1 (MQ=255)
                                                                                                                                                                                                                                aTCCCACAACAAAAATATTCCCGCCATGGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGTTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGAGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaa         <  2:1078909/239‑1 (MQ=255)
                                                                                                                                                                                                                                      tatCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggta   >  2:1003460/1‑239 (MQ=255)
                                                                                                                                                                                                                                       aacAAGAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtat  <  1:915139/237‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
AGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTAT  >  NC_000913/1575847‑1576313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: