Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,546,717 Δ1 bp 6.2% coding (453/882 nt) yddG ← aromatic amino acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,546,7170A. 6.2% 236.1 / 6.7 65coding (453/882 nt)yddGaromatic amino acid exporter
Reads supporting (aligned to +/- strand):  ref base A (35/26);  new base . (2/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

AATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTG  >  NC_000913/1546438‑1546995
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                      
aaTGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgatg                                                                                                                                                                                                                                                                               >  2:70018/1‑289 (MQ=255)
aaTGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgatg                                                                                                                                                                                                                                                                               >  1:57623/1‑289 (MQ=255)
     tGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGAttt                                                                                                                                                                                                                                                                           <  1:513014/288‑1 (MQ=255)
     tGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGAttt                                                                                                                                                                                                                                                                           <  1:547355/288‑1 (MQ=255)
        catgggcATGAAACATACCGACCATCCATGCAGCATAAGCAAAACCTAAAGTAAAAGCCGCAGAGATGAATTTAATCAGGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAAGCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcat                                                                                                                                                                                                                                                                        <  2:655693/282‑1 (MQ=255)
                  cAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTaa                                                                                                                                                                                                                                                              <  2:72475/288‑1 (MQ=255)
                     tataCCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCa                                                                                                                                                                                                                                                          <  1:448067/289‑1 (MQ=255)
                       taCCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATTTATTGATGATTTCATCATAATGTAACCCATTg                                                                                                                                                                                                                                                       >  1:122630/1‑289 (MQ=255)
                       taCCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATTTATTGATGATTTCATCATAATGTAACCCATTg                                                                                                                                                                                                                                                       >  1:618024/1‑289 (MQ=255)
                            aCATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCa                                                                                                                                                                                                                                                    >  2:71011/1‑288 (MQ=255)
                            aCATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCa                                                                                                                                                                                                                                                    >  2:253915/1‑288 (MQ=255)
                               ttCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcatca                                                                                                                                                                                                                                                                      <  2:32448/267‑1 (MQ=255)
                               ttCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcatca                                                                                                                                                                                                                                                                      >  1:32448/1‑267 (MQ=255)
                               ttCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCg                                                                                                                                                                                                                                                 >  1:117145/1‑288 (MQ=255)
                                  cATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTa                                                                                                                                                                                                                                             <  1:633334/289‑1 (MQ=255)
                                                   tCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCcg                                                                                                                                                                                                                            >  1:307683/1‑289 (MQ=255)
                                                      tAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCcgacg                                                                                                                                                                                                                         <  1:70018/289‑1 (MQ=255)
                                                                  cGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATTTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataat                                                                                                                                                                                                             <  2:618024/288‑1 (MQ=255)
                                                                  cGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATTTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataat                                                                                                                                                                                                             <  2:122630/288‑1 (MQ=255)
                                                                    cAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataataa                                                                                                                                                                                                           >  1:438063/1‑289 (MQ=255)
                                                                    cAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATACGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCTACGAGGGCtaataataa                                                                                                                                                                                                           >  1:368552/1‑289 (MQ=255)
                                                                                tAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAAt                                                                                                                                                                                               <  1:71011/289‑1 (MQ=255)
                                                                                           ggCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTg                                                                                                                                                                                     <  2:438063/288‑1 (MQ=255)
                                                                                             cGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgat                                                                                                                                                                                                                                                                                <  2:497192/195‑1 (MQ=255)
                                                                                             cGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgat                                                                                                                                                                                                                                                                                >  1:497192/1‑195 (MQ=255)
                                                                                                         cATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGt                                                                                                                                                                                                                                                      >  1:117167/1‑209 (MQ=255)
                                                                                                         cATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGt                                                                                                                                                                                                                                                      <  2:117167/209‑1 (MQ=255)
                                                                                                         cATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTa                                                                                                                                                                      <  1:315210/289‑1 (MQ=255)
                                                                                                                                      aaaCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCt                                                                                                                                         <  1:50285/289‑1 (MQ=255)
                                                                                                                                        aCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTgg                                                                                                                                       >  1:374114/1‑289 (MQ=255)
                                                                                                                                           cacaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCAGTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGcc                                                                                                                                    <  2:9056/289‑1 (MQ=255)
                                                                                                                                                    gCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGt                                                                                                                           >  1:666387/1‑289 (MQ=255)
                                                                                                                                                    gCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGt                                                                                                                           >  2:504102/1‑289 (MQ=255)
                                                                                                                                                    gCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGt                                                                                                                           <  1:253915/289‑1 (MQ=255)
                                                                                                                                                         cGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCa                                                                                                                        >  1:181975/1‑287 (MQ=255)
                                                                                                                                                          gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACg                                                                                                                                                                                                                               >  2:73301/1‑183 (MQ=255)
                                                                                                                                                          gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACg                                                                                                                                                                                                                               <  1:73301/183‑1 (MQ=255)
                                                                                                                                                          gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCa                                                                                                                     >  1:323898/1‑289 (MQ=255)
                                                                                                                                                                                            cgcgTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTcgccgc                                                                                   <  2:374114/289‑1 (MQ=255)
                                                                                                                                                                                             gcgtgcgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCt                                                                                                                                         >  2:417387/1‑234 (MQ=255)
                                                                                                                                                                                             gcgtgcgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCt                                                                                                                                         <  1:417387/234‑1 (MQ=255)
                                                                                                                                                                                             gcgtgcgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCa                                                                                  >  1:90492/1‑289 (MQ=255)
                                                                                                                                                                                             gcgtgcgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCa                                                                                  <  1:504102/289‑1 (MQ=255)
                                                                                                                                                                                             gcgtgcgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCa                                                                                  <  2:307683/289‑1 (MQ=255)
                                                                                                                                                                                                                    tgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCATGCTGATGGTTCGCCGCATACCCTACGGACAGCTCccgaca                                                           >  2:645225/1‑289 (MQ=255)
                                                                                                                                                                                                                              tGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCt                                                                                                                                                                              <  2:577596/164‑1 (MQ=255)
                                                                                                                                                                                                                              tGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCt                                                                                                                                                                              >  1:577596/1‑164 (MQ=255)
                                                                                                                                                                                                                              tGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATa                                                  >  1:394854/1‑288 (MQ=255)
                                                                                                                                                                                                                              tGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATa                                                  >  1:492410/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aTAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGa                                          >  1:13876/1‑289 (MQ=255)
                                                                                                                                                                                                                                          cGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTACCACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGTTGGGCCCAAGATAGTTCACCATACCCACTTCACTCCCCTGCTGATGGGTCGCCGCATAACCTCAGGAAAGCCCCCGACACATTTCATAACTgccatacaaa                                     >  2:472789/1‑280 (MQ=255)
                                                                                                                                                                                                                                               cAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGAc                                 >  1:487773/1‑288 (MQ=255)
                                                                                                                                                                                                                                               cAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGAc                                 >  2:472602/1‑288 (MQ=255)
                                                                                                                                                                                                                                                     aCGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGc                          <  2:117145/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         cAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACTCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACTCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGt                      >  1:108321/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         cAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTTTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATGGCTGCCGAATAACAGCCTCCCGTCGAGt                      >  2:337094/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         cAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGt                      >  2:244376/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 aaCTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGc               >  1:95488/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    tCAATTGGATGGTGTTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGGCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCttt           <  2:57623/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     cAATGGGCTGGTGGTGATAGTATGGATGATTTCATCATAATTTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAAGCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTc          <  2:666387/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aTGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGt                                                                                                                           <  1:73631/174‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aTGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGt                                                                                                                           >  2:73631/1‑174 (MQ=255)
                                                                                                                                                                                                                                                                             tggtggTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCCCTTCAATCGCCTGATGATGGGTCGCCGCATACTCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATACCAGACTCCCGGCGAGTAAATAGCCTTTCGGGCttt   >  1:535701/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                              ggtggtGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTg  >  1:267539/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                              ggtggtGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTg  >  1:685697/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                      
AATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGGCAAAGAGAATTGTCAGGCTGGGCCACAGATAGTTCACCATACCCACTTCAATCGCCTGATGATGGGTCGCCGCATACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTG  >  NC_000913/1546438‑1546995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: