Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,509,497 C→G intergenic (‑71/‑133) glk ← / → yfeO glucokinase/putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,509,4970CG98.6% 210.5 / NA 71intergenic (‑71/‑133)glk/yfeOglucokinase/putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (44/25);  minor base A (1/0);  total (45/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACA  >  NC_000913/2509357‑2509630
                                                                                                                                            |                                                                                                                                     
tGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGc                                                                                                                                     <  1:698191/143‑1 (MQ=255)
  gCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCg                                                                                                                                   <  1:921544/143‑1 (MQ=255)
    aaTGTCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGGCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGa                                                                                                                                 <  1:62335/143‑1 (MQ=255)
    aaTATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTCAGGCGATATCAAGTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGa                                                                                                                                 >  1:1192026/1‑143 (MQ=255)
         cacacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGGCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCTGGAAaggc                                                                                                                            >  1:118019/1‑143 (MQ=255)
          acgcaGAGCAAGACGTGCGTTGGTGCCGCCCACGTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggca                                                                                                                           <  1:815887/140‑1 (MQ=255)
          acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggca                                                                                                                           <  1:374832/143‑1 (MQ=255)
          acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGCGGCATAACAACAAGGCCGGAAaggca                                                                                                                           >  2:487892/1‑143 (MQ=255)
          acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGAGGGCCAAAAAACCAGGCCGGAAaggca                                                                                                                           >  2:501897/1‑143 (MQ=255)
            acaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGTAagacagg                                                                                                                         >  2:280847/1‑143 (MQ=255)
                      aCGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTGATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAACTACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTaa                                                                                                               <  1:678138/143‑1 (MQ=255)
                                gTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCt                                                                                                     <  1:270887/143‑1 (MQ=255)
                                gTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGATCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGATGCATAACAACAAAGCCGGAAAGGCAGGCTCCCAGTAAAAATCGAACt                                                                                                     >  2:1106095/1‑143 (MQ=255)
                                  gccgccCACCTCCCCGCCTAATGCTTACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg                                                                                                   <  2:721998/143‑1 (MQ=255)
                                   ccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTggg                                                                                                  >  1:286199/1‑143 (MQ=255)
                                      cccACATCACCGACTAATGCATACGTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTca                                                                                               <  2:26842/143‑1 (MQ=255)
                                        cacaTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTcaca                                                                                             >  2:988432/1‑143 (MQ=255)
                                           aTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACaa                                                                                          >  1:533270/1‑143 (MQ=255)
                                              aCCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTa                                                                                       >  1:508180/1‑143 (MQ=255)
                                               ccGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTAc                                                                                      >  1:291811/1‑143 (MQ=255)
                                                ccaCTAACGCATACTTTGTCATTCGCCAACCGCTCCGCTACAGTCAAAATAATTCTTTCACACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt                                                                                     <  1:28998/141‑1 (MQ=255)
                                                      aTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACACGGCCGAAAAGGCAGGCCCCCTGTAAATATCGATCTGGGTCACCCAATTACTTTACCg                                                                               >  2:911627/1‑143 (MQ=255)
                                                          aTACTTGGTCATTCTTCAACTGCGCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTc                                                                           <  1:603197/143‑1 (MQ=255)
                                                           tACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCa                                                                          >  1:311089/1‑143 (MQ=255)
                                                           tACTTTGTCATTCTTCAACTGCTCCGCCAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCa                                                                          >  2:809433/1‑143 (MQ=255)
                                                           tACTTTGTCATTCTTCAACTGCTACGCTAAAGTCAAACTAATTCTTTCTCACACTGTAAATACCTACGGGCATAACAACAAGGCCGGAAAGGCACGCTCCCTGTGAATATATATCGGGGTCACACAATCACCTCATCggtgaa                                                                          >  2:377648/1‑138 (MQ=255)
                                                            aCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGTCGGAAACGCCCACCCCCTGTAAATATCGCTCTGGGTCACACAATTACTTTATCGTTTCAg                                                                         >  1:273048/1‑143 (MQ=255)
                                                                 gTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCa                                                                    >  1:696990/1‑143 (MQ=255)
                                                                    aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACATGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGAACCAAtg                                                                 >  2:442684/1‑142 (MQ=255)
                                                                     ttcttcAACTGCTCCGCGAAAGTCAAAATACTTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGACCGGCAAGGCAGGCTCCCTGTAAATATCGATCTAGGTCACACAATTACACTACCCTTACAGCCACAATTg                                                                >  2:1143324/1‑143 (MQ=255)
                                                                      tcttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCACTTGc                                                               >  1:1072416/1‑143 (MQ=255)
                                                                        ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGAACCAATTGCAg                                                             >  1:72043/1‑143 (MQ=255)
                                                                        ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCCGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAg                                                             >  1:533771/1‑143 (MQ=255)
                                                                           aaCTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGCGCATAACAACAAGGCCGGAAAAGCAGGCTCTCTGTAAATATCGAACTGGGCCCAACAATTACTTTCCCGTTTCAGCACCAATTACAggtg                                                          >  2:1129023/1‑140 (MQ=255)
                                                                            ccggCTCGGCTATAGTCAAAATAATCCTTTCTCACACTGTATATACCTGGGGGCATAACACCCAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGAt                                                         <  2:82342/140‑1 (MQ=255)
                                                                            aCTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGAt                                                         <  2:259078/143‑1 (MQ=255)
                                                                                cTCCGCTAAAGTCAATATAAGTATTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGAGCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCt                                                     <  1:211678/143‑1 (MQ=255)
                                                                                 tCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtt                                                    >  1:99785/1‑143 (MQ=255)
                                                                                 tCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGACATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATAGACGCGATGCCtt                                                    >  2:447176/1‑143 (MQ=255)
                                                                                   cGCTATAGTCAAAATACTTCTTTCGCACGCTGTAAATGCCAGGGGGCAGAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTAGCGTTTCAGCACCAATTGCAGCGATGCCtttt                                                  <  1:72689/143‑1 (MQ=255)
                                                                                    gCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAGATATCGATCTGGGTCACACTATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt                                                 >  1:818744/1‑143 (MQ=255)
                                                                                    gCTAAAGTCAAAATAATTCTGTCTCACACTGTAAATGCCTGGGGGCATAACAACAAGGCCGGAAAGGCTGGCTCCCTCTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGTTGCCttttt                                                 <  2:300570/143‑1 (MQ=255)
                                                                                    gCTAAAGTAAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGCTCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt                                                 <  1:1168465/143‑1 (MQ=255)
                                                                                          atCAAAATACTTCTTTCTCACGCTGTAAATACCTGGGGGCATAGCAACAAGGCCGGAAGGGCTGGCTCCCTGTAAATAGCGATCTAGGTCACACGATTGCTTTGTCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGc                                           <  2:204144/142‑1 (MQ=255)
                                                                                             aaaaTAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGCTGCGTTTTTGCAAGCTgg                                        >  2:257150/1‑143 (MQ=255)
                                                                                                    ttctttctCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACt                                 >  1:561322/1‑143 (MQ=255)
                                                                                                       tttctCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTCCACGCTGGGCAAACTacg                              >  1:613288/1‑141 (MQ=255)
                                                                                                        ttctCCCACTGTCAATGCCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGt                             <  2:818179/143‑1 (MQ=255)
                                                                                                          cttacacTGTAATTACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCGCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCTATTGTAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt                           <  2:580827/140‑1 (MQ=255)
                                                                                                          ctcACACTGTAAATACCTGGGGGCATAACAACAAGGTCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt                           <  1:1189182/143‑1 (MQ=255)
                                                                                                           tcACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATc                          >  1:738487/1‑143 (MQ=255)
                                                                                                           tcACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATAGATCTGGGTCACACAATTACTGTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCACACTAAGTATc                          >  1:361099/1‑143 (MQ=255)
                                                                                                            ctcacTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt                         <  1:1040294/141‑1 (MQ=255)
                                                                                                            cacaCTGTAAATACCTGGGGGCATAACAACCAGGTTTGATAGGGTGGTTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTACGTATCt                         >  1:510615/1‑143 (MQ=255)
                                                                                                            cacaCTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGGTCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTGTTTGCGAGCTGGGGAAACTAAgggtct                         >  1:211850/1‑138 (MQ=255)
                                                                                                            cacaCTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCCCCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt                         >  1:1100656/1‑143 (MQ=255)
                                                                                                            cacaCTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt                         >  1:1157017/1‑143 (MQ=255)
                                                                                                             acacTGTAAATACCTTGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGGTTCAGCACCAATTGCAGCGATGCCTGTTGGCAAGCTGGCCAAACTAAGTATCTg                        >  1:38767/1‑143 (MQ=255)
                                                                                                             acacTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTGAGTATCTg                        >  1:152054/1‑143 (MQ=255)
                                                                                                             acacTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTg                        <  2:1192026/143‑1 (MQ=255)
                                                                                                               acTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTggc                      >  1:424504/1‑141 (MQ=255)
                                                                                                               acTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAc                      >  1:982022/1‑143 (MQ=255)
                                                                                                                  acaataaCCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcccc                   <  1:737550/137‑1 (MQ=255)
                                                                                                                   tAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCg                  >  1:265502/1‑143 (MQ=255)
                                                                                                                     aaTACCTGGGGGCATAACAACAAGGCCGGAAACGCAGGCCCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCa                >  2:663078/1‑143 (MQ=255)
                                                                                                                          cTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCCTAAgg           >  2:435125/1‑143 (MQ=255)
                                                                                                                            ggaggCAGAACAACAAGGCCGGAAAGGCAGGCTCCCAGTAAATAGCGATCTGGGTCACACAATTACTTTATGGTTTCAGCACCAATTGCAGCGATGCCTGTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGaa         <  1:443030/140‑1 (MQ=255)
                                                                                                                                tcATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGa     <  1:1101605/142‑1 (MQ=255)
                                                                                                                                  actacaaccagGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAACTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAc   <  2:197728/140‑1 (MQ=255)
                                                                                                                                  aTAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACACTTACTTTATCGTTTCAGCACACATTGCACCGATGCCTTTTTGCACGCTGGGCACACTAAGTATGGGACACCGAGTCAGGAACAGAAc   >  2:471880/1‑143 (MQ=255)
                                                                                                                                  aTAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAc   >  2:1122707/1‑143 (MQ=255)
                                                                                                                                   tAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCCTACGGAATAGAACa  >  2:988068/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                     
TGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACA  >  NC_000913/2509357‑2509630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: