| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 95.0% | 109.7 / NA | 41 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (18/20); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc > 2:546235/1‑143 (MQ=255) gtcgggTAGAGGTTGAGCAGCTGAGAGTTTTGGCTGGCGGGGTGGAATTTGGCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg < 2:387852/139‑1 (MQ=255) aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg > 1:541473/1‑143 (MQ=255) gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg > 2:467003/1‑143 (MQ=255) tggacggTAAGCTGCTGATATTTGTTGCTTGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGATGTATCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:61410/138‑1 (MQ=255) tAGAGGGTAAACAGCTGATCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:487426/143‑1 (MQ=255) agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc < 1:281337/143‑1 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:546736/143‑1 (MQ=255) aGCTGATATGTTTTGCTGGCGCGGTCGAATTCGTCTTTGTGCCGGCGGTAAAAGTGGAAGTACCCGGAGAATTTGTTCGGTGACGAGGATATCTCCATTTCACCGTGATTTATATGCAGGCTCTTCGTCGCCTCTTCTACttt < 1:205097/143‑1 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa > 1:188900/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTAGCCGGAGAATTTTTTCGGTGACAAGGTGATCTGAAGTTCACCGTGAGTGATATGCAGGCTCTGCGTAGCCTCTTCTACttgaca > 2:540546/1‑139 (MQ=255) tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg < 1:661535/143‑1 (MQ=255) gttGCTGTCGCGGTCTAATTTGTCTTTGTGCTTGCGCTCAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc < 1:431199/142‑1 (MQ=255) cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGGGGGATCTGCGTCGCCTCTGCTACTTTCGACGGTACGCCATa > 1:175271/1‑143 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCTGTGACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTTCGTCGCCTCTTTGATTTTTCACGGTTCGTCGTACtt > 1:151971/1‑142 (MQ=255) gTCGATTTTGTCTTGGTGCTCGCGGTAAAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTAATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGCCGTACTccacca < 1:657019/143‑1 (MQ=255) gcttGTCTGTGTGCTTTCCGTAAAAGTGGAATTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 1:434807/141‑1 (MQ=255) gTCTTTGTGCTAGCGGTAAAAGTGGAAGCACCCGGAGAATTTTTTCGGTGATGAGGTGATCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 2:957693/143‑1 (MQ=255) ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTCTCACTTTCCGCGGTGTGCCGACCCCCACCCCCATCAGCCGGaa > 2:917938/1‑143 (MQ=255) tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg < 1:201459/143‑1 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGAGTCGGCCCTTCCACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGa > 1:947563/1‑143 (MQ=255) gCTTGCGGAAAACGTGGCAGCACCCGGAGACTTTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg < 1:255161/143‑1 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTACGCCGTACTCCACCACCATCACCCGGACGATGTc > 2:535542/1‑143 (MQ=255) gTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGCCGCCTCTTCTACTTTCCACGGTACGCCGTCCTCCACCACCACCCGCAGCATGATGTCGTagt > 2:535961/1‑141 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGCCGTact > 2:881495/1‑141 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCCGATGTCCTAATc > 1:870585/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGc > 1:863076/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCGGATGTCGTAATCCTGTGc > 1:517123/1‑143 (MQ=255) ccGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAACGCGTCGTAATCCCGTCCCGCTTTCAg > 2:444135/1‑143 (MQ=255) agaATTTTTTCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTGCg > 1:499427/1‑143 (MQ=255) tttttGTGTGACGAGGTGATCTGCATGTCACCGTGATGGAGATGCAGGCGCTGCGTCGCCGCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 1:734002/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 1:395606/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 1:881055/143‑1 (MQ=255) aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTGCGCGTCCCCCAGACTCACAAACTATCCCCTTGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac < 1:444135/143‑1 (MQ=255) gcTGCATTTCACCGTGAGTGTGATGCAGGCTCTGCGTCGCCTCTTCTCCTCTCCACGGGTCGGCGTACGCCACCACCATCCGCAGCAAGACGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 2:333828/142‑1 (MQ=255) tCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTCCTACTTTCCTCGGTTCGCCGTACTCCACCACCATACGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc < 2:881489/143‑1 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGACATACACAg > 2:518178/1‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg > 1:922606/1‑143 (MQ=255) attattgttTTGCAGGGTCGGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTTCTCCACCACCATCCGCAGGTAGATGTCTTACTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACGCTGTGAAACGCCATGCCCAgg < 2:628406/140‑1 (MQ=255) gtgatAAGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTGACGAACAAACACTCTTTGAACCGCCACGCCCAGGGaac > 2:70914/2‑142 (MQ=255) atacGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc < 1:724243/143‑1 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |