| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,509,497 | C→G | intergenic (‑71/‑133) | glk ← / → yfeO | glucokinase/putative ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,509,497 | 0 | C | G | 97.9% | 142.9 / NA | 48 | intergenic (‑71/‑133) | glk/yfeO | glucokinase/putative ion channel protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (19/28); total (20/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACAT > NC_000913/2509358‑2509631 | ggCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGcc < 2:125230/143‑1 (MQ=255) aaTATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGa > 1:875299/1‑143 (MQ=255) cacacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCCCCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAAGAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggc < 2:337070/143‑1 (MQ=255) acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggca > 2:159801/1‑143 (MQ=255) acaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggcagg > 1:849669/1‑143 (MQ=255) agCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATCACACCAAGGCCGGAACGCCGGGCTcc > 1:48617/1‑143 (MQ=255) aGACGTGCGTTGGTGCCGCCCACATTACCGACTAATGCATACTCTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAACTACCTGGGGACATAACAACCAGGCCGGACAGGCAGGCTCCCTGt > 2:687132/1‑143 (MQ=255) gTGCGTTGGTGCCGCCCCCACCACCGACTAATGCACACTCTGTCATTCCTCAACTGCTCCGCCAAAG‑CCAAAATAACGCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAat < 1:307154/143‑1 (MQ=255) tGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAata < 1:18562/143‑1 (MQ=255) gCGTTGGTGCCGCCCACACCACCGACTAATGCATACTTTCTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAatat < 1:650224/143‑1 (MQ=255) gTTGGTGCCTCCCACAGCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAAGTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCg < 2:697401/143‑1 (MQ=255) tGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGAt < 1:808883/143‑1 (MQ=255) gcggccCAGATCACCGACTGAGGCATACGTGGTCAGTGTTCAACGGCTCCGCTAAAG‑TCAAGATAATTCTGGCTCAGACTGTATATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg < 1:684017/140‑1 (MQ=255) cccACATCACCTACTAATGCATACTTTGTCATTCTTCAACTGCTCTG‑TATTGATCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTca < 1:216862/143‑1 (MQ=255) tccccGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt < 1:805550/140‑1 (MQ=255) tacccGACTAGTGCATACTTTGTCATTCTTCAACTGCGCCGCTAAAG‑TCAAAATAATTCTTTCTCACACGGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt < 2:436224/140‑1 (MQ=255) cGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt < 1:467377/143‑1 (MQ=255) aCTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACttt > 1:116473/1‑143 (MQ=255) cTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAGCAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTa > 1:442111/1‑143 (MQ=255) gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt > 1:474246/1‑143 (MQ=255) aCTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAg < 1:765493/143‑1 (MQ=255) tCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCaa > 2:297685/1‑143 (MQ=255) tCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCaa > 1:164331/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtt > 1:103068/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGACACACGATTACTTTATGGTCTCCGCagctcta > 1:943206/1‑137 (MQ=255) cttcAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCa > 2:373523/1‑143 (MQ=255) tcAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGTCCGGAAAGGCAGGCTCCCTGTAAATATAGATCTGGGTCACACAATTACCTTATCGTTTCAGCACCAATTGCAGc > 2:352161/1‑143 (MQ=255) caCTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGa < 1:477334/142‑1 (MQ=255) tGCCCCGCTAAAG‑CCACAACAAGTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGTCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc < 1:15385/143‑1 (MQ=255) tCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtt > 1:840889/1‑143 (MQ=255) gCTAATG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt < 2:253964/143‑1 (MQ=255) cTAAAG‑TCAAAATAATTCTTTCTCACACAGCCCGGAGCTGCGCGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTg < 2:925091/143‑1 (MQ=255) tCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCt < 1:159801/143‑1 (MQ=255) tCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGACGCCTCTTCGCAAGCt > 2:7837/1‑143 (MQ=255) acataatTGTGTCTCACCCGTTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTggg < 2:813257/141‑1 (MQ=255) aaaTAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGGTggg > 1:736974/1‑143 (MQ=255) tattctCACATGGTAAATTCCTGGGGGCATAACGACACGGCCGGAATGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 2:624132/141‑1 (MQ=255) tttctCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg < 2:474246/143‑1 (MQ=255) ctcACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt < 1:373523/143‑1 (MQ=255) tcACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCCCCCTGTAAATATCGATCTGGGTCACACAATTACTTTACCGTTTCAGCACAAATCGCAGCGATGCCTCTTTGCAAGCTGGGCACACTAAGTagc > 2:248804/1‑141 (MQ=255) cattGTAAATTCCTTGGGGCATAACAACAAGTCTGTAAAGGTAGGCTCCCTGTAAATATCGATCTGGGTCACATAATTACTTTATCTTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGa < 2:663947/140‑1 (MQ=255) cTGGAAAGACCCGGGTGCATAACCACTGGGCCGGAAATGTGGTCGTCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcc < 2:925701/143‑1 (MQ=255) tACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATa < 2:116473/143‑1 (MQ=255) aCCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATAGATATGGGGCACAAACTTACTTTACTGATTCAGCACCAATTGCAGCGATGCCATTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATac > 2:833012/1‑142 (MQ=255) tcTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAg < 2:103068/142‑1 (MQ=255) cTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATaatg > 2:760521/1‑141 (MQ=255) ggCATAACACCAGGGCCGGAAAGGAAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAg < 1:157955/143‑1 (MQ=255) tAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACa < 1:7837/143‑1 (MQ=255) aacaacaagGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAAAt > 1:5866/1‑143 (MQ=255) | GGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACAT > NC_000913/2509358‑2509631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |