Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 741,501 Δ1 bp 6.2% coding (1056/1482 nt) dtpD ← dipeptide and tripeptide permease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913741,5010A. 6.2% 238.4 / 6.8 65coding (1056/1482 nt)dtpDdipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base . (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

GATCGCCCCGGAAGCATCAAACGAAGCCTGCGATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTT  >  NC_000913/741213‑741781
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                        
gATCGCCCCGGAAGCATCAAACGAAGCCTGCGATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggcca                                                                                                                                                                                                                                                                                          >  1:454666/1‑289 (MQ=255)
     ccccGGAAGCATCAAACGAAGCCTGCGATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggccaaggcc                                                                                                                                                                                                                                                                                     >  2:152678/1‑289 (MQ=255)
                tCAAACGAAGCCTGCGATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAAtt                                                                                                                                                                                                                                                                          >  2:121334/1‑289 (MQ=255)
                              cGATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcg                                                                                                                                                                                                                                                             <  2:204946/288‑1 (MQ=255)
                               gATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCa                                                                                                                                                                                                                                                           <  1:152678/289‑1 (MQ=255)
                                   tCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGt                                                                                                                                                                                                                                                       >  1:236432/1‑289 (MQ=255)
                                            cAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTAc                                                                                                                                                                                                                                               >  1:207953/1‑288 (MQ=255)
                                             aaTCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTcccc                                                                                                                                                                                                                                                                     <  1:12440/265‑1 (MQ=255)
                                             aaTCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTcccc                                                                                                                                                                                                                                                                     >  2:12440/1‑265 (MQ=255)
                                             aaTCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTAccc                                                                                                                                                                                                                                             <  2:207953/289‑1 (MQ=255)
                                               tCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcg                                                                                                                                                                                                                                                             >  1:140160/1‑271 (MQ=255)
                                               tCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcg                                                                                                                                                                                                                                                             <  2:140160/271‑1 (MQ=255)
                                                     ccgccAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACAct                                                                                                                                                                                                                                     <  2:236432/289‑1 (MQ=255)
                                                      cgccAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTCCGATTACCCGCGACAct                                                                                                                                                                                                                                     >  2:436906/1‑288 (MQ=255)
                                                                   gcgATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTaccacc                                                                                                                                                                                                                        >  2:175211/1‑288 (MQ=255)
                                                                                     agcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCg                                                                                                                                                                                                      <  1:412418/288‑1 (MQ=255)
                                                                                         tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGcaca                                                                                                                                                                                                  >  2:364163/1‑288 (MQ=255)
                                                                                           tAGATGCCGGTTAATACGCCGGTAAAACCGGGGATGTCAATGCGCGTAAGTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGacac                                                                                                                                                                                                                                      <  2:564236/250‑1 (MQ=255)
                                                                                            aGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCAt                                                                                                                                                                                              >  1:285046/1‑289 (MQ=255)
                                                                                                     ttAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAACTTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTac                                                                                                                                                                                                                            >  1:564236/1‑250 (MQ=255)
                                                                                                     ttAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGacac                                                                                                                                                                                                                                      <  2:111547/240‑1 (MQ=255)
                                                                                                     ttAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGacac                                                                                                                                                                                                                                      >  1:111547/1‑240 (MQ=255)
                                                                                                     ttAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGa                                                                                                                                                                                      <  1:121334/288‑1 (MQ=255)
                                                                                                               ggTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGCTCCGCACGGTACGATTACCCGCGACCACCTCTTTTACCACCCACGCCCGGAACACACCGCACAGCATCAATGCGAAGGCCATAAt                                                                                                                                                                            >  2:100781/1‑288 (MQ=255)
                                                                                                                            aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATc                                                                                                                                                               <  1:364163/288‑1 (MQ=255)
                                                                                                                               tCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCgg                                                                                                                                                           >  2:473873/1‑289 (MQ=255)
                                                                                                                                   tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCgg                                                                                                                                                       <  2:157466/289‑1 (MQ=255)
                                                                                                                                      gcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGaa                                                                                                                                                     <  1:175211/288‑1 (MQ=255)
                                                                                                                                       cgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGc                                                                                                                                                                                                                   <  1:535637/225‑1 (MQ=255)
                                                                                                                                       cgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCCCTTTTACCACCCACGc                                                                                                                                                                                                                   >  2:535637/1‑225 (MQ=255)
                                                                                                                                                      ggCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCg                                                                                                                                                                                                      >  2:32053/1‑223 (MQ=255)
                                                                                                                                                      ggCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCg                                                                                                                                                                                                      <  1:32053/223‑1 (MQ=255)
                                                                                                                                                      ggCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTACGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACata                                                                                                                                    >  1:436981/1‑289 (MQ=255)
                                                                                                                                                                          gTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGaa                                                                                                                                                     >  2:589964/1‑252 (MQ=255)
                                                                                                                                                                          gTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGaa                                                                                                                                                     <  1:589964/252‑1 (MQ=255)
                                                                                                                                                                          gTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTc                                                                                                                >  2:207684/1‑289 (MQ=255)
                                                                                                                                                                            tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGt                                                                                                                 <  1:139141/286‑1 (MQ=255)
                                                                                                                                                                            tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTc                                                                                                                <  1:197794/287‑1 (MQ=255)
                                                                                                                                                                                cAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACCTATCGCGGTTAACGAAGCGGTCGAtata                                                                                                          >  1:228087/1‑289 (MQ=255)
                                                                                                                                                                                     cccATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCAAGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCCGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAAACAAACCTATCCAGGTTAACGAAGCGGTCGATATaaca                                                                                                       >  2:32132/1‑285 (MQ=255)
                                                                                                                                                                                          cacaGCCGGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACCTTGCGGACCAGCGGGCGCTTAAGGTCAGAAGGCAGAAGCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATGACCCGCGACACTCGCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATc                                                                                                <  2:454666/289‑1 (MQ=255)
                                                                                                                                                                                                    ccTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCg                                                                                       <  1:473873/288‑1 (MQ=255)
                                                                                                                                                                                                             aTCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGt                                                                                                                 <  2:350001/253‑1 (MQ=255)
                                                                                                                                                                                                             aTCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGt                                                                                                                 >  1:350001/1‑253 (MQ=255)
                                                                                                                                                                                                              tCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATcgc                                                                                                                                                             <  1:102459/208‑1 (MQ=255)
                                                                                                                                                                                                              tCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATcgc                                                                                                                                                             >  2:102459/1‑208 (MQ=255)
                                                                                                                                                                                                              tCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGc                                                                            >  1:315604/1‑289 (MQ=255)
                                                                                                                                                                                                              tCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCg                                                                           >  1:538784/1‑289 (MQ=255)
                                                                                                                                                                                                              tCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCg                                                                           >  1:213621/1‑289 (MQ=255)
                                                                                                                                                                                                               cAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGa                                                                          <  2:213621/289‑1 (MQ=255)
                                                                                                                                                                                                               cAGTGGCAGAGAAGAGTGACCATACAGTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGa                                                                          <  2:538784/289‑1 (MQ=255)
                                                                                                                                                                                                                          aagaGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGa                                                                 >  1:110602/1‑288 (MQ=255)
                                                                                                                                                                                                                              gggACCATACATTGCGGACCAGCGGGCGGTTAAGGTCAGAATGCAGAATCCGTCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGaaca                                                              <  1:100781/285‑1 (MQ=255)
                                                                                                                                                                                                                              gTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGaaca                                                              <  1:207684/287‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcaCCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGCTTACCCGCGACACTCACTTTTACCACCCACGCCAGGAACACACCGCACAGAATAACTGCGAAGGCATTAATCGACTGAAACATCAGGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGTACCGGACGATATATAGGCTAAGCCGCGTGCCGCCCTATTGTGAGAAAGCCCAAAACAACAACCTgaagaattt                                              >  2:409365/4‑286 (MQ=255)
                                                                                                                                                                                                                                               aCCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGa                                            <  2:110602/288‑1 (MQ=255)
                                                                                                                                                                                                                                                  aGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGaa                                                                                                                      >  1:33497/1‑211 (MQ=255)
                                                                                                                                                                                                                                                  aGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGaa                                                                                                                      <  2:33497/211‑1 (MQ=255)
                                                                                                                                                                                                                                                    cGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTACTCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTc                                       >  1:475241/1‑288 (MQ=255)
                                                                                                                                                                                                                                                      ggCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGcc                                                                                     <  1:71246/240‑1 (MQ=255)
                                                                                                                                                                                                                                                      ggCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGcc                                                                                     >  2:71246/1‑240 (MQ=255)
                                                                                                                                                                                                                                                             aaGGGCAGAATGCAGAATCCGGCGCTCACCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGcc                             <  1:283783/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aaTGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTcccc                                                                                                                                                                                                                                                                     >  2:35161/1‑49 (MQ=255)
                                                                                                                                                                                                                                                                     aaTGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTcccc                                                                                                                                                                                                                                                                     <  1:35161/49‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCCCCTTCCg                >  2:4928/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                              tCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGt            <  1:4928/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        atcaGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGtttt  >  2:172300/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                        
GATCGCCCCGGAAGCATCAAACGAAGCCTGCGATGTCTGATCGGCAATCACGCCCGCCAGATAGTTCGCGATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCAGGAACACACCGCACAGCATAACTGCGAAGGCATTAATCGACTGGAACATCGCGGTCGGAACGGTATAACCAAACATATCGCGGTTAACGAAGCGGTCGATATAAAGGCTAATCGAGCTACCGCCCTGTTGTGCGAAGGCCCAGAACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTT  >  NC_000913/741213‑741781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: