| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 67,890 | +TG | coding (448/1503 nt) | araA ← | L‑arabinose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 67,887 | 1 | . | G | 92.9% | 68.0 / 0.0 | 28 | coding (451/1503 nt) | araA | L‑arabinose isomerase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (14/12); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
| * | NC_000913 | 67,887 | 2 | . | T | 92.6% | 73.0 / ‑0.2 | 28 | coding (451/1503 nt) | araA | L‑arabinose isomerase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (14/11); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
TTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑GT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGA > NC_000913/67753‑68018 || tttATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑GT‑‑gtgacc > 1:648278/1‑138 (MQ=255) aTCGGTGACCGCCACTTCACGCATTTTATCTCCAAATCGACAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCAc < 1:393801/143‑1 (MQ=255) tGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCCCGGCAt > 1:472690/1‑143 (MQ=255) tctctaCTTCACGCTTGTTATCGTCATATCGGTAGACTTTCATATGACGGGTATTCTGTTTAGTGTCCGCTTGATGTATCTAGTAGCCGATACGCTCATGGGCTTGTTTATCCTGTCAGTGT‑‑GTCCGGTAACCACGGCATGtt < 2:605099/138‑1 (MQ=255) ccACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACGACGAAATGATGCt > 2:25/1‑143 (MQ=255) cACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGCTGCCGGGTATCCTGTATAGCGACCGCCTGACGCATCCAGGGGCCGATACGCTCATGGGCTTGTTTAACCTGCCAGTGT‑‑GACCGGTAACCACGCCATGctgctg > 2:319381/1‑143 (MQ=255) gctgcACGCATGGTATCGCCAAATCGGCAGACTTGCAGGTGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGa < 2:611711/139‑1 (MQ=255) tCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCTATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAAGCACGGCATGTTGCTGACGc > 1:361284/1‑143 (MQ=255) gTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATAcgcgc < 1:606392/143‑1 (MQ=255) gCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGaa > 2:101658/1‑143 (MQ=255) gTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCCCCAttttca > 1:718083/1‑138 (MQ=255) tccgttttAGGGACCGCCGGACTCATCCGGTACCCGTTACGCTCATGGGCTTGTTTATCCTGCCAGGGT‑‑GACCGGTAACCACGGCATGTTGCTGCCGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTc < 2:76710/138‑1 (MQ=255) tGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCCGCCTgg > 2:305453/1‑143 (MQ=255) gttggacaCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggtt < 2:427988/136‑1 (MQ=255) agagACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCCATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttcac > 1:447444/1‑142 (MQ=255) cgcccGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttcaggt < 2:611397/140‑1 (MQ=255) agaCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCGTGTGCAGTCTggttaagcg > 1:125869/1‑141 (MQ=255) gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATa < 2:19388/143‑1 (MQ=255) aCGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc < 1:25/143‑1 (MQ=255) gCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATACTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCCATGAAGCCGAACTCGCGACCGCCATGTGCATCCTGGTTTAAGTTCTTAAAGTCCa > 1:464828/1‑143 (MQ=255) gAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAg > 2:232093/1‑131 (MQ=255) gAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAg < 1:232093/131‑1 (MQ=255) gATACGCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCCATGAAGCCGAACTCGCGCCCGCCATGTGCCGTCTGGTTCTGCTCCTTATCGTCTATATAGATaatata > 1:311862/1‑138 (MQ=255) atgCTCATGGGCTTGTTTATCCTGCCAGTGT‑‑GACCGGTAACCCCGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCa < 1:320620/141‑1 (MQ=255) gCTCATGGGCTTGTTTATCCTGCCA‑‑GTGGGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACg < 1:614640/143‑1 (MQ=255) ggCCTGTTCATCCTGCGAGTGT‑‑GGCCGGTAACCGCGGCATGTTGCTGCCGCATGCGCGCGCCAATGAAGCCGCACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgcgc < 2:658222/143‑1 (MQ=255) gTTTATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACGGTCCCACGGCAGCGCCCCGt > 1:380385/1‑143 (MQ=255) tATCCTGCCAGTGT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGa > 1:27684/1‑143 (MQ=255) || TTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑GT‑‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGA > NC_000913/67753‑68018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |