| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,967,533 | A→G | L301P (CTG→CCG) | ptsP ← | PEP‑protein phosphotransferase enzyme I; GAF domain containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,967,533 | 0 | A | G | 97.3% | 95.0 / NA | 37 | L301P (CTG→CCG) | ptsP | PEP‑protein phosphotransferase enzyme I; GAF domain containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/16); minor base C (0/1); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GACAACACCGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATT > NC_000913/2967394‑2967665 | gACAACACCGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCATGATGATTCAGCAATCGCTGACCCGgcg > 1:627275/1‑143 (MQ=255) aacaCCGACTAAGCGATCCTGGGGCAGCCCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCAc < 1:302260/143‑1 (MQ=255) gACTGAGCGAGCCTGGGGCAGCTCAGCAGGCGGTGCCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAAt < 2:623012/143‑1 (MQ=255) cTAAGCCATCCTGGGGCATCTCAACAAGCGGCGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCg < 2:186942/143‑1 (MQ=255) gCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAg > 2:411645/1‑143 (MQ=255) tCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGCTCGGCCCTTGATTAGCGCCATCAAGCTGAAACAGCAATCGCTGACCCGGCGCCCCGAACTCGCCAGCCCg > 2:260499/1‑143 (MQ=255) tGGGGCAGCTCAGCAAGCGTTGTCGTTGACAGTTCATCTGCTACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGCTGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGctc < 1:474377/143‑1 (MQ=255) cTCAGCAAGCGTTGTCGCTAACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTGCGGCCCCTGATTAGCGTCATCAAGATGAAACAGAAAACGCTGACCCGGCGCACGCAAATCGCCAGCGCGCGCTTCGAGAt > 2:553546/1‑143 (MQ=255) gCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGCGAtgctt > 1:692502/1‑139 (MQ=255) aaGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTAGGCCCTAGATTAGCGTCATCAAGCTGAAACAGCAATCACTGAATCGACGCACGTAAATCGCCCGCTCGCTCCTTGAGATactcct > 2:315295/1‑138 (MQ=255) cggccAGTTCATCCGCCACCAGAACGACACCTTCCGGCCAGGCGTCCGGCCCTTGATTAGCCTCATCAAGATGAAACAGCAACCGCCGCCCCCGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGcgcg < 1:251698/139‑1 (MQ=255) tGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCgg > 1:16522/1‑143 (MQ=255) tCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCCAACTg > 1:691294/1‑143 (MQ=255) gCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGCGATAGTTATCACTTAGCGCGGCAAACTGTTCGGc < 1:137057/143‑1 (MQ=255) gCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTCACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGCTAGGTATCACTCAGCGCGGCCAACTGTCCGGc > 2:562517/1‑143 (MQ=255) ccaccaGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGGTAGTGATCACTTAGCGGGGCAAACTGTGCGGCa > 2:103173/1‑143 (MQ=255) caccaGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCTAACTGTTCGGCaa > 1:672628/1‑143 (MQ=255) caccaGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCACACTGTTCGGCac > 1:299678/1‑142 (MQ=255) accaGAATGAAACGTTCCGGCCAGGCGTTCGGACCTTAATTAGCGTCATCCAGATGAAACAGCAATCCCTGACCCGGCGCACGTCACTCGCCAGCCCGCTCTTTCAGATACTTACCACCTAGCGCGCCCACCTGTTCCGCaca > 2:57715/1‑141 (MQ=255) aTGAAAGGTTCTGGCCAGCCGTGCGGCCCTTGATTAGCGTCAGCAAGATGAAACAGCAGTCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc < 1:256831/143‑1 (MQ=255) gAAACGTGCCGGCAAGGCGTTCGGCCCTTGATTAGCGTCATCAAGAGGAAACAGCAATCGCTGTCCCGGCGCCCGTAAATCGCCAGCCCCCTCTTTGAGATAGTTATCACTTCGCGCGGCAAACTGTTCCGCAAATTTTTCaa < 1:390774/143‑1 (MQ=255) tCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCCCGTAAATAGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAGTTTTCAATGACCGt > 1:57692/1‑143 (MQ=255) ggCCAGGCGTTGGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGtttt < 1:139606/143‑1 (MQ=255) ccAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTa > 2:293915/1‑143 (MQ=255) ccAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTa < 1:21936/143‑1 (MQ=255) cAGGCGTCCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAAGCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCCAACTGTCAGTCAAATTTTTCAATGACCGTTTTTAc < 2:627275/143‑1 (MQ=255) gCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGCTAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTATTCAATACCCGTTTTTACCGc > 1:405473/1‑143 (MQ=255) cTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGCGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGcc > 1:559455/1‑143 (MQ=255) ttGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCa > 1:312246/1‑143 (MQ=255) gaGCGTCATTGAGATGAAACAGCAATCGCTGACCCGGCGCGCGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAg < 2:157148/142‑1 (MQ=255) catcaACATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTa < 1:411645/143‑1 (MQ=255) tcgaggtGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATc < 2:568867/137‑1 (MQ=255) aGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCACTGACCGTTTTTACCGCCCCCTCTGCCACCGAGCCTTTATCAAc > 2:282514/1‑143 (MQ=255) aGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAAc < 2:16522/143‑1 (MQ=255) aGATGAAACAGCAATCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAAc > 2:657572/1‑143 (MQ=255) acccaTCGCGGCCCCGGCGCACGTAAATTGCCAGCCCGCTCTTTGAGATACTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCaaa < 1:200173/139‑1 (MQ=255) tCGCTGACCCGGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAAtt < 1:293915/143‑1 (MQ=255) | GACAACACCGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATT > NC_000913/2967394‑2967665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |