| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,591 | C→T | Q375* (CAG→TAG) | yrfF → | putative RcsCDB‑response attenuator, inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,591 | 0 | C | T | 97.8% | 122.0 / NA | 47 | Q375* (CAG→TAG) | yrfF | putative RcsCDB‑response attenuator, inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (23/22); minor base G (0/1); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT > NC_000913/3527451‑3527728 | aaGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGACTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAg < 2:79688/143‑1 (MQ=255) aacaCTTTCCGTTACAGCACTGTCTGCTCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCAtt < 2:259284/140‑1 (MQ=255) gagcTTTCCGGGACAGCACTGGCGGCGCAGTACGATTATCGCTGCGGGCTCGCTGATGGTGCTGTTTATGCTGGTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTg < 1:406901/140‑1 (MQ=255) aagcTTTCCGTTGCGGCACGGGGTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTg < 2:554275/140‑1 (MQ=255) aaaCTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTGATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCACTCATCGATGAAAGGCGCGTAGACCATTg > 2:482771/1‑143 (MQ=255) cTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCAGTTATTCTGGGTCCCGCTGGATATGCCGCAGCAATCCACTCACTCATGGATGAAAGGCGAGTAGACCACTGAAg > 2:278177/1‑143 (MQ=255) ccGTTAGAGCACTGGCTGCGCAGTACGATTACCGCTGCGGGCTCGCTGCGGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCCCTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac < 1:677567/143‑1 (MQ=255) ccGTTACTGCCCTGTCTTCACAGTACGATTATCGCGGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac < 2:424932/143‑1 (MQ=255) cACTGGCTGCGCAGTACGCTTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTaaa < 2:20522/143‑1 (MQ=255) tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCCATGAAGCCCCCAACGTAAaaca > 2:602614/1‑143 (MQ=255) tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAaaca < 1:281308/143‑1 (MQ=255) tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCACGCTGGATATGCCGCTGCAATTCGCTCTCTCATGGGTGAAGGGCGCGTAGACCATTGAAGCCGCCAGGGTAAaaca > 1:341517/1‑143 (MQ=255) ggcggcGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCGACCAGCGTAAaacaa < 2:605066/143‑1 (MQ=255) gCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAaacaac < 2:712078/143‑1 (MQ=255) ctccgcAGTTCGATTCTCGCTTCGGGCTCGCTACTGGTTCTGTTTATGCTGTTATTCTGGATCCCGCTGAATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACt < 2:487992/140‑1 (MQ=255) gactATCGCTGCGGGCTCGCCGCTGGTGCTGTGTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTg < 1:174328/140‑1 (MQ=255) gATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATCTCAGCCACCAGCGTAAAACAACCGGCTGAAGCTg > 2:415312/1‑143 (MQ=255) cTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGAGCCCGCTGGATATGCCGCTGGAGTTCACTCTCTCATGGATGAAAGGCGCGTAGACCGTTGAAGCGAGCAGCGGAAGACACCGGGGTGGCGGTGGCGTGAgg > 2:2186/1‑143 (MQ=255) gcgcgcTCGCGGCTTCTGCTGTTTATGCTGTTAGTCTGGATCCCGCTGGATATGCCGCTGAAATTCACGCCCTCCGGGATGCAACGCGCGTCGACCATTGAAGCCGCCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGt < 2:38253/143‑1 (MQ=255) gCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTTTCATCGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGGCAAACAACTGGCTGTCGCTGCCGTGCGGCTCGGc > 1:66056/1‑143 (MQ=255) cTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAACGGCGCGTAGACCATTGAAGCAACCAGAGAAAAACAACTGGATAACGCTGGCGTGTGGGTAGGCg > 1:228013/1‑143 (MQ=255) cTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTCAACCATTGAAGCCCCCAGCGTACCACAACTGGCTGACGCTGGCGTGCGGGCAGGcc > 2:568174/1‑142 (MQ=255) tCGCGGCTGGTGCTGTTTATGCTGTTATTCTGGCTCCCGCTGTATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGa < 2:43554/143‑1 (MQ=255) gTGCTGTTTATGCTGTTATACTGGAGCGCACTGGGTTTGGCTCTCAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑Cg < 1:518530/143‑1 (MQ=255) tGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGt > 2:149696/1‑143 (MQ=255) gCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTa > 1:691716/1‑143 (MQ=255) gTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAATGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTAGCTGAAGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTa > 1:10711/1‑143 (MQ=255) tttATGCTGTTATTCTAGATCCCGCTGGAGATGCCGCTGAAATTCACTCTCTCATGGATGAACGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑Cggcgtag > 1:690442/1‑137 (MQ=255) acgCTGTTACTCTGGATCCCGCTCGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGCCCCTCGACGCCACTAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCgg < 1:268699/141‑1 (MQ=255) tGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGGACg > 1:33341/1‑143 (MQ=255) gTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACgg < 1:87698/143‑1 (MQ=255) gTTATTCTGGACCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCCTTGAAGCCACCAGCGTAAAACAACTGGCGGACGCTGGCGTGCGGGAAGGCGATAGACTGCCGTA‑TAGCGGTACgg > 2:248837/1‑143 (MQ=255) cTGGATACCGTTGGATATGCCGCTGAAATTCACGCTCTCGTGGCTGGAAGGCGCAGAGACGGTTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAAtgt < 1:300536/143‑1 (MQ=255) tGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAAtgtg > 1:154119/1‑143 (MQ=255) ggacgCCGCTGGTATTCACTCTCTCGTGGATGCGGGGCGGGTGGCCCATTGAAGCCACCAGCGTAAGACAACTGGCTGGGGCGGGTTTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACt < 2:471713/139‑1 (MQ=255) cGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAAATGGCTGACGCTGGCGCGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATACGAACTTCCGGa > 2:307215/1‑143 (MQ=255) ttCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTCAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGCACTTCCGGAACCTGGAg > 2:267063/1‑143 (MQ=255) tCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAgc > 2:673149/1‑143 (MQ=255) tCACTCCCTAATGGATTAAAGGCGCGTAGCCCATGGAGGCCACCAGCGTAAAACAACTGGCTGACGCGGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAgc < 2:113735/143‑1 (MQ=255) ctctctCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAACATTCGAAGTTCCGCAACCTGGAGcgcg > 1:697296/1‑143 (MQ=255) tctctcATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAAGGTGAAATATTAGAACTTCCGGAACCAAGAgcgcaa > 2:50963/1‑141 (MQ=255) ctctcATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGaa < 1:369434/143‑1 (MQ=255) ccaaTGGCTGAACGGCGCGTAGGCCATTGGCGCCACCAGCGTAAAACAACTGGCGTACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATGAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGaaaa < 2:29401/140‑1 (MQ=255) gATGAAGGGCGCGTAGCGCATTGAAGCCACCAGCGTACAGGAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAAtt < 1:227471/143‑1 (MQ=255) catacacggCGCGCAGCCCATGAAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAAtt < 2:610751/136‑1 (MQ=255) aTGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAGTATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTc > 1:447239/1‑143 (MQ=255) gAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑AGCATTAGTCCCACTTGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCACTTccc > 1:163435/1‑141 (MQ=255) gcgcgtAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCtttt < 1:149696/143‑1 (MQ=255) | AAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT > NC_000913/3527451‑3527728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |