Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	73.2 / NA	26	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/13);  total (13/13)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132650‑2132924                                                                                                                                          |                                                                                                                                          cccggTAGAGGGCACACAGCTGGTATTTTGTGTTTTCGCGGCCGGTTTTTTCGTTGTTCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                      <  2:282798/140‑1 (MQ=255)    tgTAGAGGTTAAACAGCTGATATTTTTTGCTGGTGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                   <  2:307222/142‑1 (MQ=255)    ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATGTGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTc                                                                                                                                   >  2:423496/1‑143 (MQ=255)     gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                  >  2:221053/1‑143 (MQ=255)      tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                 <  1:614923/143‑1 (MQ=255)      tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGAAACGAGGTGAACTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                 >  1:111268/1‑143 (MQ=255)        gatggTAACCCGCTGTTCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct                                                                                                                               <  2:293495/140‑1 (MQ=255)                      gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGCGGTGCTCTGCATTTCACCGCGATTGCTCTGCAGGCTCTGCGTCGCCTCTTCTACTTTcccc                                                                                                                 >  1:273068/1‑141 (MQ=255)                          tttgttGCTGGCGCGGTCGAATTGGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                             <  2:32187/143‑1 (MQ=255)                                      gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCTACGGTTCGCCGTACTcca                                                                                                 >  2:42577/1‑143 (MQ=255)                                                    cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAgg                                                                                   <  1:435298/143‑1 (MQ=255)                                                     tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGa                                                                                  >  2:385540/1‑143 (MQ=255)                                                            ttGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                           <  1:468594/143‑1 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGtca                                                                       >  2:681602/1‑141 (MQ=255)                                                                     aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCtg                                                                  >  1:671088/1‑143 (MQ=255)                                                                     aaGTGGAAGTACCCGCAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCCGGCAGATGACGTAATCCtg                                                                  >  2:602982/1‑143 (MQ=255)                                                                      aGTGGAAGTCCCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                 <  1:214362/143‑1 (MQ=255)                                                                           aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc                                                            <  1:221053/143‑1 (MQ=255)                                                                                                ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGGAGGCACTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCTCTTTCACTTCTGTATCGaacaa                                       >  1:619292/1‑143 (MQ=255)                                                                                                 gTGACGTGGTGCTCTGCATTTCACCGAGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                      <  2:596113/143‑1 (MQ=255)                                                                                                             tCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCCCAGGAAGCTGTCGTAATCCTGTGCTGCTTTCAGTTCGCTCTCGAACAAACACTCTTTgccc                          >  1:342279/1‑140 (MQ=255)                                                                                                                    ttCACCGTGATTGCTACGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAGCGAACAAACACTCTTTGAAACGCCATg                   <  2:289210/143‑1 (MQ=255)                                                                                                                    ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTGCTCCGCCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg                   <  2:566309/143‑1 (MQ=255)                                                                                                                       aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCCGGACGATGTCGTCCTCCTGTGCCGCTTTCAGTTAGGTATAGAACAAACACTCTTTAAAAAGCCATGcac                >  1:148184/1‑141 (MQ=255)                                                                                                                                   atGCAGGCTCTGCGTCGCCTCTTCTACTTCCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt    <  1:151559/143‑1 (MQ=255)                                                                                                                                     gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAACCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTTTGAAACGCCATGCCCAGCTAAAGACCCgg  >  1:22443/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                          TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132650‑2132924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑