Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,992,705	IS5 (+) +4 bp	coding (1553‑1556/2547 nt)	cyaA →	adenylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	33 (0.740)	29/282	0.6	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3992705 =	0 (0.000)					coding (1553/2547 nt)	cyaA	adenylate cyclase
*	?	NC_000913	= 2290113	NA (NA)	59 (1.330)	39/282	0.1	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3992708	0 (0.000)					coding (1556/2547 nt)	cyaA	adenylate cyclase

TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATC  >  NC_000913/2288778‑2289056                                                                                                                                              |                                                                                                                                          ttttATATGCTGTTGTCAGCCAGGATTGATTTTCGTATTATTGGCGGTGCCATAAAAAAGATGGATATATTTTTCCAGGACAACACAATAAAAAGTATAACAATACTTAACAGTTGATGGCAATGATAGTGGAGAAAAAaagg                                                                                                                                          >  2:649215/1‑143 (MQ=255)        tgctgtgctgCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTGTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGc                                                                                                                                   <  2:758298/143‑1 (MQ=255)        tGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGc                                                                                                                                   <  2:585225/143‑1 (MQ=255)        tGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGc                                                                                                                                   <  1:617474/143‑1 (MQ=255)        tGCCGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATCGACCAGCTGGATATCTTTTTCCAGGACAACACCATCAGCTGTCTCACCATTCTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGc                                                                                                                                   <  2:397554/143‑1 (MQ=255)                tcCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGc                                                                                                                           >  1:636995/1‑143 (MQ=255)                   gCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGCAGGTGCGAATAAGCggg                                                                                                                        >  1:472992/1‑143 (MQ=255)                   gCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTAGGGGTGAATATAAGGAAGGTGCGAATAAGCggg                                                                                                                        >  2:66780/1‑143 (MQ=255)                     cAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAACCGGGGa                                                                                                                      >  2:453648/1‑143 (MQ=255)                       ggATTTATTTTCGTCTTATTCGCGGTCCCATAAACCATCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                    <  1:41559/143‑1 (MQ=255)                              ttttCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATtcttct                                                                                                             >  1:533112/1‑143 (MQ=255)                                       tattGGCGGTCCCATAAACAAGCTGGATATCTTTTTCCAGGACAACACCATCCACTGTCTCACCATCCTCAACAGTTGAAGCCCATGCTATGGGTGAATATAAGGAAGGTGCGAATACGCGGGGAAAGTCTTCTCGCCCGAca                                                                                                    >  2:228685/1‑142 (MQ=255)                                         ttGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                  >  2:682169/1‑143 (MQ=255)                                                  ccATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGgcatttca                                                                                         >  1:448348/1‑143 (MQ=255)                                                   cATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                        >  1:373893/1‑143 (MQ=255)                                                    aTAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGttatttcatg                                                                                       >  1:713534/1‑142 (MQ=255)                                                     tAAACCAGCTGGATTCCCTTTTCCCGCACAACACCACCAACTGTCTCACCATACGCAACAGTTGATGCTAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                                                                      <  1:270867/143‑1 (MQ=25)                                                        aCCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                   <  1:174001/143‑1 (MQ=255)                                                        aCCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATCCTATGGGTGAAAATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCCCGGCTGACTCAGTCATTTCATttctt                                                                                   >  2:622184/1‑143 (MQ=255)                                                          cAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCa                                                                                 >  2:529863/1‑143 (MQ=255)                                                                 atatCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGa                                                                          <  1:655073/143‑1 (MQ=255)                                                                  tatCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGCAGAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCTGTTCCTTTCTTCATGTTCGAg                                                                         >  2:45330/1‑143 (MQ=37)                                                                       ttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                                    >  2:254187/1‑143 (MQ=255)                                                                            cAGGACAACACCCTCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                               <  2:42854/143‑1 (MQ=255)                                                                            cAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATCAGGAAGGTGCGAATAAGCGGGGAAACTCTTCTCGGCTGACTCAGTCATTTCATTACTCCATGTTTGAGCCGAttgttt                                                               >  2:55766/1‑143 (MQ=25)                                                                            cAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                               >  1:772219/1‑143 (MQ=255)                                                                            cAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                               >  2:613989/1‑143 (MQ=255)                                                                             aGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                              >  2:748699/1‑143 (MQ=255)                                                                                      ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                     <  1:423080/143‑1 (MQ=255)                                                                                      ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATAGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCATGTaaa                                                     >  2:374224/1‑143 (MQ=255)                                                                                            aCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                               <  1:32867/143‑1 (MQ=255)                                                                                              tGTCTCACCATACTTAGCGGTTTATTCCAATGTTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                             <  2:267684/143‑1 (MQ=17)                                                                                                      cATACTCAACAGTTGATGCCAAACATATGAGTGAAGAGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATGTCTTCATGGTTGAGCCGAGTTTTTCTCCCGTAAATACCTTGAATTAGcca                                     >  2:488173/1‑142 (MQ=14)                                                                                                           tCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                                >  1:706905/1‑143 (MQ=255)                                                                                                              aCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCGGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAGTCCGCCTATTTAGAc                             >  1:236338/1‑143 (MQ=255)                                                                                                               cAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                            >  1:432593/1‑143 (MQ=255)                                                                                                                   tGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAACTCCCTTGAATCAGCCTATGTAGATCGttc                        >  2:635741/1‑142 (MQ=255)                                                                                                                     aTGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttct                      <  1:40908/143‑1 (MQ=255)                                                                                                                      tGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGCACTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttctt                     >  1:32275/1‑143 (MQ=255)                                                                                                                       gCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTAATTTCATTTCTTCATGTTTGAGCGGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTGTTTGGACGGTttctcc                    >  1:352449/1‑141 (MQ=14)                                                                                                                         cAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGACTACCTCAGTAATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATTCATTGAATCCACCCATTTAAAACGTTTCTTAGc                  >  2:124056/1‑143 (MQ=14)                                                                                                                          aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc                 >  1:410334/1‑143 (MQ=255)                                                                                                                          aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGACTTTTTCTCCCGTAAATGCCTTGCATCAGCCTATTTAGACCGTTTCTTCGcc                 >  2:639164/1‑143 (MQ=255)                                                                                                                               tATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCCTCATGTTTGAGACCATCCCAGCGATCGTGGATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa            >  1:182171/1‑143 (MQ=14)                                                                                                                                      gAATATAGGGAAGATGCGAATAAGCGGTGAAATTCTTCTCGGCTGACTCATTCATTTCATTTCTTCATGTTGGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt     <  2:121506/143‑1 (MQ=11)                                                                                                                                      gAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTCTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt     <  2:373893/143‑1 (MQ=18)                                                                                                                                       aaTATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTa    >  1:737152/1‑143 (MQ=32)                                                                                                                                         tataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc  <  2:741786/143‑1 (MQ=31)                                                                                                                                         tataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc  >  1:730167/1‑143 (MQ=31)                                                                                                                                              |                                                                                                                                          TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATC  >  NC_000913/2288778‑2289056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.