| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 43.9% | 20.0 / 62.8 | 67 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (23/14); new base T (19/10); total (43/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC > NC_000913/4295920‑4296202 | aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc < 2:583030/143‑1 (MQ=17) gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCAAGAAATAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt > 1:673308/1‑143 (MQ=11) gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAAGCAGCGCAATAGCCAGATTTAGCGTGATTTTCTACGTCGGATAACGCGTGTACCCCGCCTCCGACATCCAGGCCTGa > 2:170923/1‑143 (MQ=11) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc > 1:417661/1‑143 (MQ=17) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCCATGCCTGATGCgcc > 2:800720/1‑141 (MQ=12) ccccATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg < 1:523272/140‑1 (MQ=17) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGACGCGCCg > 2:26338/1‑143 (MQ=12) gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATTCCTTATGCGACGCt > 1:202133/1‑143 (MQ=12) aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg > 2:289662/1‑143 (MQ=17) tCCGACATGAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTAGGCGAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACGTCAACGCCTGATGCGACGCTTGc < 1:181944/143‑1 (MQ=11) ccGACATCAACGCCTGATGCGACGCTAAAAGCATCTGATTAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCGGACATCAATGCCTGATGCGACGCTTGcc > 2:59193/1‑143 (MQ=11) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTGCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGCTTACTCCGCATCCGACATCAACGCCTGATGCGCCGCATGCcgc > 2:523272/1‑143 (MQ=11) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACACCTGCcgc > 1:621922/1‑143 (MQ=255) gACATCAACACCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAGCAATGCCTGATGCGACGCTTGCcgc > 2:770259/1‑143 (MQ=255) aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg > 1:682427/1‑143 (MQ=21) tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑t > 2:753728/1‑143 (MQ=21) tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTCGCCGCGCC‑t > 2:753410/1‑143 (MQ=25) cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑tt < 1:125866/143‑1 (MQ=21) cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑tt > 2:744652/1‑143 (MQ=37) taggCCTGATAAGACGCGTTAAGCGTCGCATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTCATTTTGTAGGTCGGATGAGGCGTTTACGCCGCATCCGACATAAATGCCAGATACGACGATTGCCGCGTC‑tca > 1:660262/4‑141 (MQ=11) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTa > 1:323064/1‑143 (MQ=255) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTa > 2:259594/1‑143 (MQ=38) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCGATGCATGATGCGACGCACGCCGCATC‑TTa > 2:350275/1‑143 (MQ=11) aCGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGCAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTC‑TTAt < 1:660033/143‑1 (MQ=255) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAAGCTTGGCGCGTC‑TTATCAGGc > 2:758494/1‑143 (MQ=255) cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGcga > 1:375432/1‑141 (MQ=38) gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGcccg > 2:547822/1‑141 (MQ=255) ttAACGCGTCGTATCAGGCGTACGCCAGCCCGCGCGATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTTCCGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGAc < 2:573287/143‑1 (MQ=11) cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTCGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCATCATC‑GGCCTACGCCATACGGTGCa > 2:314041/1‑143 (MQ=11) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACGGCGCAAtgt > 1:653161/1‑141 (MQ=12) cgggCCGACGCCTGACAGCGCAATAGCCTGATTTGCGGTGATTTTGTGGGTCGGATCGGGCGTTTACGCCGCGTCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTg < 2:655427/141‑1 (MQ=14) cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑TTATCAgg > 2:36499/1‑117 (MQ=12) cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑TTATCAgg < 1:36499/117‑1 (MQ=25) cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTg < 1:259594/143‑1 (MQ=39) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt < 1:614952/143‑1 (MQ=38) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa < 1:306287/93‑1 (MQ=34) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt < 1:548827/95‑1 (MQ=21) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt > 2:548827/1‑95 (MQ=21) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑TTATCa > 2:312644/1‑107 (MQ=12) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑TTATCa < 1:312644/107‑1 (MQ=25) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACCCCAAACAGCGCATTAGCCTGAATTACCGt > 2:252788/1‑143 (MQ=11) ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg > 1:583030/1‑143 (MQ=17) aGACAGCGCAATAGCCTGATTTAGCGTCATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAt < 1:583019/143‑1 (MQ=255) gagcgcgcTATGGCCTGACTTCGCGTGATTTTGTAGGTCGGACAGGGCGTTTCCGCCGCGTCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGTAATAGCCTGATTTAGCGTGAtt < 2:489441/139‑1 (MQ=11) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa < 1:384472/83‑1 (MQ=35) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑tt > 2:142701/1‑93 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑tt < 1:142701/93‑1 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTACGCGTCACTTTGTAGCTCGGac > 2:192667/1‑142 (MQ=14) tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTC‑TTATCAgg < 2:348140/84‑1 (MQ=255) tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAgg > 1:348140/1‑84 (MQ=255) gCGTGATTTTGTGTGTCGGGTAGGGCGTTTCCGCCGGGCCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt < 2:403682/143‑1 (MQ=11) gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGc > 2:722979/1‑85 (MQ=255) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc < 1:65392/125‑1 (MQ=40) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc < 1:329007/52‑1 (MQ=255) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc > 2:329007/1‑52 (MQ=255) tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGGATcc > 2:797442/1‑143 (MQ=255) gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCa > 2:83773/1‑143 (MQ=39) ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCCCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg < 2:417661/143‑1 (MQ=37) ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAg > 2:171672/1‑48 (MQ=40) ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg < 1:744652/143‑1 (MQ=35) cccGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACATCCACGCCTGATGCGACGCTTaa > 1:606997/2‑143 (MQ=255) aTCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTGTGCCGCAGCCGACATCCACGCCTGGTGCGACGCTTCAcggggg > 1:64423/1‑139 (MQ=11) aTCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGGCGCGTc > 2:265033/1‑143 (MQ=21) cAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTAAGCCGCATCCGACACCAATGCCTGACGCGACACTTAACTCGTCtg > 2:424392/1‑142 (MQ=11) cAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa < 1:47053/110‑1 (MQ=33) aaCGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGGTTGTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACGTCAACGCCTGATGCCACGCTTCACCCTTCTTa > 1:281418/1‑143 (MQ=11) aaCGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa > 2:482136/1‑143 (MQ=32) cGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATCTTGTAGGTCGGCTACGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGAGTCGTagc > 2:311825/1‑141 (MQ=11) | AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC > NC_000913/4295920‑4296202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |