| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,509,471 | G→A | intergenic (‑45/‑159) | glk ← / → yfeO | glucokinase/putative ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,509,471 | 0 | G | A | 98.4% | 181.4 / NA | 62 | intergenic (‑45/‑159) | glk/yfeO | glucokinase/putative ion channel protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (34/27); minor base C (0/1); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAC > NC_000913/2509332‑2509610 | gccTTAGCCCGCGCGTTTGCACCACTGGCAATACCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATaa < 2:573421/141‑1 (MQ=255)gTCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATaa > 1:611004/1‑143 (MQ=255) tCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATaaa > 1:481833/1‑143 (MQ=255) gCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTTCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCt < 2:581587/143‑1 (MQ=255) ccTGCGAGAATTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGAATACTTGGTCATTCTTCAACTGCTCCGCTACAGTCAAAATAATTATTTCTCAAACCATAAATGccgg > 2:627350/1‑141 (MQ=255) cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCt < 2:433741/141‑1 (MQ=255) cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCt > 1:433741/1‑141 (MQ=255) tGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTggg < 1:943122/143‑1 (MQ=255) cGAGATTGCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCACTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTggggg < 1:932607/143‑1 (MQ=255) gagaTTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACATGGGGGc > 1:427469/1‑143 (MQ=255) tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaa > 2:837915/1‑143 (MQ=255) ttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaac < 1:713945/143‑1 (MQ=255) tCACCACTGGCAATATCACACCGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaaca < 2:539017/143‑1 (MQ=255) tatCACACAGAGCAAGACGTGCGTTGAAGCCCCCCAAATCACTGACTAGTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGaaa > 1:835752/1‑143 (MQ=255) cacaGAGCAAGGCGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggcag < 1:221786/143‑1 (MQ=255) cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggcaa > 2:1040569/1‑142 (MQ=255) cacaGAGCAAGACGTGCGTTGGTGCCGCACACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCTGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACATGGGGGCATAACAACAACGCCGGaacgacac > 2:450877/1‑137 (MQ=255) agagCAAGACGTGCGTTGGTGCCGCCCACATCACCGACGAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATACTTCTTTCCCACACAATAAACACCTCGGCGCATACCAACACCCCCGGAAAGCCAGCCt > 2:252223/1‑143 (MQ=255) agCAAGACGTGCCTTGGTGCCCTCCACATCACCGACTAATGCATACTGTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTcc < 2:899522/143‑1 (MQ=255) aGACGTGCGTTGCTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGt < 2:712418/143‑1 (MQ=255) ttGGTGCCGCCCGCATCAGCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGa < 2:1044123/143‑1 (MQ=255) gTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGTGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCt < 2:125795/143‑1 (MQ=255) ccACATCACCGACTAATGCATAGTTTGTCTGTTTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATAACGATCTGGGTcac > 2:647993/1‑143 (MQ=255) cACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAtt > 2:436330/1‑143 (MQ=255) ggcTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATGCGTTCGCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt < 2:84624/141‑1 (MQ=255) gACTAATGCATACTTTGTCCTTCTAGGACGACGCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt < 1:946188/143‑1 (MQ=255) gACTAATGCATACTTTGTCATTCTTCAACTGCTCCTCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCTTCTATTCATTCGTGATCGATCTGGGTCACACAATTACtt < 2:587064/143‑1 (MQ=255) gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt < 2:848091/143‑1 (MQ=255) aTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATAGCCGGTGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATGTGGGTCACGGCATTACGATATCg > 2:231144/1‑143 (MQ=255) aTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCg > 1:739520/1‑143 (MQ=255) gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt < 1:736311/143‑1 (MQ=255) aTACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTGTCACACTATAAATACCTGCGGGCTTAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTc > 1:132450/1‑143 (MQ=255) aCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAACGGCAGGCTCCCGGTAAATGTCGATCTGGGTCACGCAATTACTTTATCGGTTCAg > 1:157280/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGACAGGCAGGCTCCCTGTACATATCGATCCGGGTCACACAATTACGTTCTCGTTTCAGCACCAAtt > 2:833640/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAACGGCAGGCTCCCTGCCCCTCTCGCTCTGGCTCCCACACTTACTTTTTCGTTCCATCCCCAAtt > 1:405760/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtt > 1:175461/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATATGGGTCACACAATTACTGCACGGTTTCAGCATCAAtt > 2:610454/1‑143 (MQ=255) ttcttcAACCGCTCCGCCAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTg < 1:70332/143‑1 (MQ=255) ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAg > 1:795151/1‑143 (MQ=255) tcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGc > 1:455726/1‑143 (MQ=255) tcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGc > 1:220936/1‑143 (MQ=255) tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGCACATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc > 2:157166/1‑143 (MQ=255) tCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATCGCAGCGATGCCtt > 1:458943/1‑143 (MQ=255) tCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCAGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTAAAGTTTCAGCACAAATTGCAGCGCTGCCtt > 1:228705/1‑143 (MQ=255) tcTACAGACAAGATAATTCCTTCTCACACTCTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt < 2:786214/142‑1 (MQ=255) gCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACCAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACTATTACTTTATCGGTTCAGCACCAATTGCAGCGATGCCttttt < 2:122285/143‑1 (MQ=255) aaaaTAACTCTTTCTCACACTATAAATACCTGGGGGCATAACAACACCGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg < 1:853230/143‑1 (MQ=255) tctttcgCACACTATAGATACCTGGGATCCTTACAACAACGCTGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTa < 1:441161/143‑1 (MQ=255) tctctctcACACAATCAATACCCGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATTGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTa < 1:627350/143‑1 (MQ=255) ctttctCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTGATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 2:13409/143‑1 (MQ=255) ctgtctcACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 2:611004/140‑1 (MQ=255) tttctCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg > 1:790706/1‑143 (MQ=255) tttccgacacTATAAATCCCTGGCGGCATAACAACAACGCCGGAAAGGCATGCTCCCTGTAAATATCGAACTGGGTCACACAATCACTTTATCGTTTAAGCACCAATTGCAGCGATGCCTTCTTGCACGCTGGGCAAACTaac > 2:185859/7‑142 (MQ=255) ctcACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCAGCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt > 2:336622/1‑143 (MQ=255) tcACACTATACATACCTCGGCGCATAACAACAACGCCGGAAAGCCAGGCTCCCTGTAAATACCGACCTGGGTCACACAATGACTCTGCCGCTTCAGCAGCACGTGCAGCGAAGCCCGTGTGCACGCCGGGCCAACTAAGTGTc > 2:1062529/1‑143 (MQ=255) tcACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCACACTAAGTATc > 1:167060/1‑143 (MQ=255) cacaCTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 1:845367/143‑1 (MQ=255) cacaCTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATAGTGCTGGTTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 1:776729/143‑1 (MQ=255) cacaCGATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 1:501803/143‑1 (MQ=255) acacTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTg > 1:589572/1‑143 (MQ=255) acacTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATAGCGATCTGGGCCACACACTTGCGTCATCGTTGCAGCGCCAATTGCAGCGAGACCTTTTTGCACGCTGGGCAAACTAAGTATcag > 2:251052/1‑141 (MQ=255) acTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAc > 1:705068/1‑143 (MQ=255) | GTCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAC > NC_000913/2509332‑2509610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |