| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,977,580 | T→G | S328A (TCA→GCA) | galR → | galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,977,580 | 0 | T | G | 95.6% | 122.9 / ‑5.2 | 47 | S328A (TCA→GCA) | galR | galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (26/17); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAA > NC_000913/2977441‑2977716 | gCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAg < 1:5388/143‑1 (MQ=255) tCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGCAATCACTAATGGCTTTAGTGCGACGCTGGTACGTCGTCATGCAgtgt > 1:217/1‑143 (MQ=255) gcCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACt < 1:857929/143‑1 (MQ=255) accGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCt > 1:480042/1‑143 (MQ=255) gTGCGTTACCCAAGCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGa < 1:353034/143‑1 (MQ=255) tgctgttcccAATCGTGACTATGCCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAg < 1:18607/136‑1 (MQ=255) tGCGTTACCCAATCGTGACGCTGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAg < 2:1032386/143‑1 (MQ=255) tGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGACATCACTAATGTCTTTAGTCCGACGCTGGTCCGTCGTCAGGCAGTGTCAACTCCGTccgtgggg > 2:865431/1‑136 (MQ=255) gCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAgg > 1:500834/1‑143 (MQ=255) gTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTAGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGGCTTTAGTCCGACGCTGGTAAGTCGTCACGCACTGTCAACTCAGTCGAGGGACGCa > 2:383774/1‑143 (MQ=255) cccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAGGtc > 1:87528/1‑143 (MQ=255) ccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACGCCGTCGCTAGaggcaaggca > 2:248879/1‑143 (MQ=255) ccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTATCTAGTCAGACGCCGGTACGTCGCCACGCCGGGTCACCTCCGCCGCTGGAGGCAAGtca > 2:700474/1‑143 (MQ=255) tCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTcatcat < 1:122838/143‑1 (MQ=255) tGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCAGGAAATCAATAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTTGAGGCAAGTCATCATGcc > 1:131670/1‑142 (MQ=255) aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGGCGCTGGAAAGCCGTCACTCTCTGTCAACGTCGTCGCTGGAGGCAAGTCATCATGCAAc < 1:450524/143‑1 (MQ=255) tGGCGACCCAGGCTGTCGAACTGGCTTTGGCGCTGTCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGc < 2:670138/143‑1 (MQ=255) gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGa < 1:46042/143‑1 (MQ=255) cGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGAc < 1:842202/143‑1 (MQ=255) cGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGGACGTCGTCATGCAGTGTCAACTCCCTCCCTCGACGGACGCCCCCATGCAACCCGCGAc > 2:780245/1‑143 (MQ=255) gACCCAGGCTGCCGAACTGGCTTTGGAGCTGGCGGATAATCGCCCTCTCCCGGAACTCACTAATGTCTTTAGTACGACGCTGGTCCGTACTCATTCAGTGTCAACTCCGCCTCTGTAGGCAATCCATCATGCAACCAgagtcg > 2:585736/1‑138 (MQ=255) tGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt > 1:1061374/1‑143 (MQ=255) tGGCTTTGGCGCTGGCGGATACTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCACCTCCCTCGCCGCAGGCAAGTCATCATGCAACCAGCGACCAACCGCAGTTCAAGcca > 2:308102/1‑141 (MQ=255) tGGCTATGGCGCGGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTATTCCGCCGCTGGTACGTCGTCATACAGTGTCAACGCCGTCGCTGGAGGAAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCaa < 1:128959/143‑1 (MQ=255) tttGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGGCAGGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACAAACCTCAGTTACAGCacgtcc > 2:789926/1‑138 (MQ=255) ttgggCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTcc < 2:387630/140‑1 (MQ=255) cgcTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAACCAAGTCTAgcgc > 1:389316/1‑143 (MQ=255) cTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCa < 1:4364/143‑1 (MQ=255) tGGCGGTGAAGCGCGCGCGCCCGGGAGTCACGAATGGCTTTAGTCCGACGCGGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAg < 2:41671/143‑1 (MQ=255) tGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAg > 1:1036553/1‑143 (MQ=255) gCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCCTGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGtc > 2:902850/1‑142 (MQ=255) aaTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttctt > 1:706908/1‑143 (MQ=255) cGCCCTCTCCCGGAAATCACTAATGTCTTTACTCCGACCCTGGTACGTCAACATCCAGTGTCAACTCCTTCGCCGCAGGCAAGTCATCATGCAACCCGCGACTAACCGCAGTTACAGTAATCCCATCTCCATGAAttctccca > 2:112474/1‑139 (MQ=255) ctctCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGt > 1:267742/1‑143 (MQ=255) ccGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCACGTCATCATGCCACCAGCGACTAACCGCAGTTAAAGCAATTCCCGCGCCAGTAATTCTTCGGTGGCCTGGc > 1:424140/1‑143 (MQ=255) gAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGAc < 2:500834/143‑1 (MQ=255) gAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGAc < 2:601544/143‑1 (MQ=255) ttCACTGATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGTAAGCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGc < 2:516574/142‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTCCGATGGTCTGGCGACGGCGaa > 1:66605/1‑143 (MQ=255) gTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGCCTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTg > 1:126966/1‑143 (MQ=255) tCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa > 1:414977/1‑143 (MQ=255) cGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCCTCATGCAACCAGCGACTCACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAcc > 2:857487/1‑143 (MQ=255) gatgCTGGCACGTCGTCATGGAGGGTCAACCGCGTCGCTGGAGGCAAGTCAGCATGCAACCAGCGACTAACCGCAGTGAAAGCAATCCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa < 1:284018/140‑1 (MQ=255) gACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCCGCGACTAACCGCAGTTAAAGCACTTCCAGCGCCAGTCATTCTTCGATGGTCTAGCGACGGCGAATCAACCGCGCCTAACCa > 2:814744/1‑143 (MQ=255) gACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTgcaca > 1:407312/1‑139 (MQ=255) gACGCTGGTACGTCGTCATGCAGTGTCAACTCCGACGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa > 1:639221/1‑143 (MQ=255) cgtcATGCGGTGTCTCCTCCGTCGCGGGAGGCAAGTCAGCATGCAACCAGCGACTAACCGCCGGTAAAGCAATCCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGaa < 2:1007722/143‑1 (MQ=255) | GCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAA > NC_000913/2977441‑2977716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |