| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,267,597 | A→G | *73* (TAA→TGA) | tdcR → | L‑threonine dehydratase operon activator protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,267,597 | 0 | A | G | 89.7% | 139.9 / 7.3 | 60 | *73* (TAA→TGA) | tdcR | L‑threonine dehydratase operon activator protein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (33/19); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01 | |||||||||||
CTATTTGTTGGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAG > NC_000913/3267457‑3267736 | ctttttGTTGCACAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATgaa < 2:411759/140‑1 (MQ=255) tATTTGTTGGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCCTAAAt > 1:953700/1‑143 (MQ=255) ttgGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATtcttct > 2:100538/1‑143 (MQ=255) ggAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTcc > 1:717265/1‑143 (MQ=255) gagaACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTccc > 1:723002/1‑143 (MQ=255) agaACAACAATCTATTTTATCAATATTTTAAAATCTCGCTTCTACGCATGGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTcccc < 1:770225/143‑1 (MQ=255) gaACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCt < 2:264565/143‑1 (MQ=255) acaaacaTTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCa < 2:71683/143‑1 (MQ=255) caaacaTTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCAt < 1:547337/143‑1 (MQ=255) atttatttTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTATCTTCTGCCAGGACGTCAGTTTGTGAGTGCCACTGTTTTCCATGAATTGTTCTCGCCTCATAGGcg > 2:589836/1‑143 (MQ=255) tttattttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGcgc > 1:700966/1‑142 (MQ=255) tttattttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGcga < 2:947166/143‑1 (MQ=255) tttattttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGcga > 2:662061/1‑143 (MQ=255) ttttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGaa < 1:919958/143‑1 (MQ=255) tcttatCAATATTTTAAAATTTCGAATACATGTATTGATCAGCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGaa < 1:408947/141‑1 (MQ=255) ttatGAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATa < 2:303905/143‑1 (MQ=255) ttatCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTCCCATGAATTCTTCTCCCCTCATAGGCGACGAATa < 2:665789/143‑1 (MQ=255) tatTGTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTGCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCAtttt < 1:259565/143‑1 (MQ=255) tttAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTtgtg < 1:859071/143‑1 (MQ=255) tttAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTtgtg > 2:53518/1‑143 (MQ=255) tttAAAATTTCGAATACATGTATTGATCATCGCGAACAATTGATTAACGTCAACTTTTTCGCTTCTGACAGGACGTCATTTTGTGAATGAAATCGTTTTCCATGGATTATTATCTCATCATTGTCGACGGATATCATTTggtg > 2:562963/1‑143 (MQ=255) tAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGtt > 1:891802/1‑143 (MQ=255) tttCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGa > 2:1009698/1‑143 (MQ=255) ttCTAATATATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGCATGCCATTGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGAt < 1:99418/143‑1 (MQ=255) aaTACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGTCGAATAGCATTTTTTGTGGAgcctccca > 2:744527/1‑136 (MQ=255) aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACaaaa < 1:937220/143‑1 (MQ=255) aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTATGTTGAGGATCAAAAAAca > 2:80716/1‑142 (MQ=255) gtgtcgttcatcTCCACCACTTGATGAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaa < 2:518189/136‑1 (MQ=255) tATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGGATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATCGGCGACGAAGAGCATTTTGGGTTGGGGATGACAGGACGaag > 1:256338/1‑142 (MQ=255) tATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaat > 1:42382/1‑143 (MQ=255) gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAAtt < 2:717265/143‑1 (MQ=255) atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTGGAGGATCCCAAAACAAATAATTg > 2:850688/1‑143 (MQ=255) tcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGCCAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCGTCATAGGCGACGAATAGCATTTTGTGTTGAGGATGACAGACCGAATAATTgt > 1:271697/1‑142 (MQ=255) tctcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGa > 1:747348/1‑143 (MQ=255) tctcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGa > 2:603486/1‑143 (MQ=255) gAACAATTGATTAACGTCAACTTTTTCTCTTCAGACAGGACGTCATATTGTGAATGAAATCGTTTTCGATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGATGAGGATCACAAAAAGAATAATTGCTGAtacg > 2:534786/1‑140 (MQ=255) taCAATTGATTAACTTCAACTTTTTCTTTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgcc < 2:382665/142‑1 (MQ=255) aaCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgcc > 1:1005377/1‑143 (MQ=255) aaTTGATTAACGTCAACTTTTTCTCTTCTGACATGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCcgcg > 2:646664/1‑143 (MQ=255) aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTGGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAg < 2:1009185/143‑1 (MQ=255) aaCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTc < 1:603486/143‑1 (MQ=255) accttttCTCTTCTGAGAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa < 2:544959/140‑1 (MQ=255) tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagaccaaga > 1:130033/1‑143 (MQ=255) tctcTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaac > 1:745176/1‑143 (MQ=255) cttctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAg > 2:171404/1‑143 (MQ=255) ctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGa > 1:839231/1‑143 (MQ=255) ctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGa > 1:505168/1‑143 (MQ=255) gACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGaaa > 1:593719/1‑143 (MQ=255) gACGTCATTTTGTGAATGCAATCGTTTTCCATGACTTCTTCTCCCCTCATAGGCGACGAATAGCATTTGGTGTTGAGGATCACAAAAAGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTc < 1:329724/143‑1 (MQ=255) gACGTCATTTTGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATGTTc > 2:125134/1‑143 (MQ=255) ttGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCGCAGAGCtt > 1:171778/1‑143 (MQ=255) ttGTGAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATGGCTGATCGCCGCGATAGGGTCAGACGAAGACAACAAGGGAAACTTTCGCGGAGCtt > 1:92542/1‑143 (MQ=255) tgtgAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCttt > 1:798383/1‑143 (MQ=255) gAATGCAATCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAAAGAATAATTGCTGATCGCCGCGATAAGGTCACACGATGACAACATGGAACACTTTCACACAGCTTTTGa > 2:901385/1‑143 (MQ=255) aaaTGGAATCGTTTTCCATTAATTCTTCTCCCCGCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGa < 2:1022772/142‑1 (MQ=255) tGCAATCGTTTTCCATTAATTCTTCTCCTCTGATAGGCGACGTGTCAATATTTATAGTGAAAACAACTATACGTATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCg < 2:470766/143‑1 (MQ=255) aaTCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCg > 1:459594/1‑143 (MQ=255) aTCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAACGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt > 1:268953/1‑143 (MQ=255) tCGTTTTCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTa < 1:80716/143‑1 (MQ=255) tttCCATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGcc > 2:100471/1‑143 (MQ=255) cATGAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAg < 2:240976/143‑1 (MQ=255) | CTATTTGTTGGAGAACAAACATTTATTTTATCAATATTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAG > NC_000913/3267457‑3267736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |