Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G98.5% 197.9 / ‑3.4 65intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (22/42);  total (22/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTG  >  NC_000913/3560314‑3560585
                                                                                                                                               |                                                                                                                                   
tCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcg                                                                                                                                     >  2:374528/1‑142 (MQ=255)
tCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATCGcg                                                                                                                                     >  1:87073/1‑142 (MQ=255)
  gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCCCCGCCCCGCCATGATGGcgca                                                                                                                                   >  1:1082722/1‑140 (MQ=255)
  gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                                   >  1:916902/1‑140 (MQ=255)
  gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                                                                                                   <  1:896093/143‑4 (MQ=255)
   aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcag                                                                                                                                  >  1:228508/1‑139 (MQ=255)
   aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcag                                                                                                                                  <  1:631206/143‑5 (MQ=255)
      acctgCGTCAGGCCATTGGGGATGTTCCCCGCCACTTTGCGGGCGATCCGCTCCTTTTCTTCGGTCTGGGTGGCCTTGCGACCGTGCCACGCCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                                                               <  2:318122/139‑1 (MQ=255)
        cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCa                                                                                                                            <  1:557744/143‑1 (MQ=255)
            gTCGAGCTATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtt                                                                                                                        <  1:1207485/143‑1 (MQ=255)
             gcgtgCCATTGGGGTCTTCGTCCGCCGCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                       <  2:1309911/139‑1 (MQ=255)
              cGAGCCCTTGGGGAGTTGCTCCGCCACTTTGCGGGCGCTGCGCTATATTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtttt                                                                                                                      <  1:253778/143‑1 (MQ=255)
               gAGCCATTGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                     <  1:221938/143‑1 (MQ=255)
                 gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCt                                                                                                                   <  1:612472/143‑1 (MQ=255)
                   gagtGGGGTTGTGCCCCGTCTGTTTGTGGTCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATGGGGCCACGGCGTGTTAACCGAACTGGCCGGCCGTGTCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTcc                                                                                                                 <  2:1333084/140‑1 (MQ=255)
                    aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTGTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCAGCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCg                                                                                                                >  1:18332/1‑143 (MQ=255)
                     ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAACGCAGCGCCGCACCGCCATGCTGGCGCAGGCGCGGGTTTTGCTcctc                                                                                                               >  2:579970/1‑141 (MQ=255)
                        gggATTTGCTCCGCCACTCTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGAGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                            <  1:861808/143‑1 (MQ=255)
                         ggATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                           <  1:699396/143‑1 (MQ=255)
                          gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                          >  1:1318785/1‑143 (MQ=255)
                            tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCa                                                                                                        >  1:421814/1‑143 (MQ=255)
                                cTCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                    <  1:209405/143‑1 (MQ=255)
                                 tCCGGCACTTTGCGGGCGAGGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                   <  1:574597/143‑1 (MQ=255)
                                   cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCACTGAggc                                                                                                 >  2:277360/1‑142 (MQ=255)
                                      cACCTTGCGGGCGATGCGCTCTTTTTCGGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                              <  1:235966/143‑1 (MQ=255)
                                       aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTCTTGCTCCGCCACCCCGTTGAGGTcgcg                                                                                             >  2:899967/1‑143 (MQ=255)
                                        ctgtGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                            <  1:785124/140‑1 (MQ=255)
                                         tttACGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                           <  1:875800/143‑1 (MQ=255)
                                         tttACGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                           <  1:874447/143‑1 (MQ=255)
                                           tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAAGATAGCGCAGGATCAGGGTTTGCTCCGCCAGCCCATAGCCGTCGCGGCGa                                                                                         >  2:304569/1‑143 (MQ=255)
                                            gCGGGCGATGCGCTCTGTTGCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                        <  2:1098250/143‑1 (MQ=255)
                                              gggCGATGCGGTCCTTTTCTTCGGGCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                      <  1:1115328/143‑1 (MQ=255)
                                              gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                      <  1:1114515/143‑1 (MQ=255)
                                                gCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                    <  1:659978/143‑1 (MQ=255)
                                                      cgcgCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                              <  1:1240542/143‑1 (MQ=255)
                                                      cgcTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                              <  2:301650/143‑1 (MQ=255)
                                                       gctgtggttcttcGGTCTGGGTGTCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                             <  2:655226/136‑1 (MQ=255)
                                                         tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCAGGCt                                                                           >  2:865541/1‑143 (MQ=255)
                                                         tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                           >  1:1091704/1‑143 (MQ=255)
                                                           tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTgc                                                                         >  2:114654/1‑142 (MQ=255)
                                                           tttttCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa                                                                         <  1:855360/143‑1 (MQ=255)
                                                                    gggcgggggggCCTTGGGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                <  2:1095636/138‑1 (MQ=255)
                                                                    ggTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                <  2:238187/143‑1 (MQ=255)
                                                                     ggcgggggggCCTTGCGCTCGTGCCGCGGCGTGTGAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGGTCGGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                               <  2:232739/139‑1 (MQ=255)
                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCCGGATCCAAGTAGCGGTTTGCTTCTCATCGCGGTCGCGGCGAGTAGTCTGCGGGCTGACGGAGAAATg                                                               >  1:310172/1‑143 (MQ=255)
                                                                        tGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGACGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGACTGctc                                                            >  1:1061668/1‑143 (MQ=255)
                                                                         gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                           <  1:811818/143‑1 (MQ=255)
                                                                         gggTGGCCTGGCGATCGTGCCACGGCGGGTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                           <  1:884523/143‑1 (MQ=255)
                                                                            tgtccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                        <  1:1323540/140‑1 (MQ=255)
                                                                            tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTCGTCTGCGGGCTGGCGGAGAAATGCTCTAcc                                                        >  1:320558/1‑143 (MQ=255)
                                                                              gCCTTGCGCTCGTGCCACGGCGTGTTAACCGTACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                      <  2:493121/143‑1 (MQ=255)
                                                                               ccTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcccgg                                                     <  1:1227819/143‑4 (MQ=255)
                                                                                     gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                               <  1:161994/143‑1 (MQ=255)
                                                                                       tCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                             >  1:442005/1‑143 (MQ=255)
                                                                                         gggCCACGGCGTGTTGACCGCACTGGAAGACAGGGCCGCACCGCCATGATGGCGCGGGATCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                           <  2:182840/141‑1 (MQ=255)
                                                                                           gCCCCGGCGTGTCAACCGGACTGGAAGGCAGCGCCGCACCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                         <  2:838824/143‑1 (MQ=255)
                                                                                           gCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                         >  1:448529/1‑143 (MQ=255)
                                                                                            ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAACTGCTCTACCAGCTCTTCGGCACTgg                                        >  2:714963/1‑142 (MQ=255)
                                                                                             cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                       >  1:919324/1‑143 (MQ=255)
                                                                                             cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTCTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCATAGTCTGCGGGCTGCCGGAGCAGTGCTCCACCAGCTCTTCggaccgggc                                       >  2:864758/1‑136 (MQ=255)
                                                                                                       tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCACAGCGGTACTGACATAAGCcagc                             >  2:131483/1‑143 (MQ=255)
                                                                                                            gagcTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAACTCTTCGGTACTGACATAACCCTGCTGttt                        <  1:1080066/140‑1 (MQ=255)
                                                                                                            gAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGAGAAGAGTCTGCGGGCTGACGGGGAAATGCGCTCCCAGCTCGTCGGTACTGACATAACCCTGGGGttt                        >  1:506479/1‑143 (MQ=255)
                                                                                                               cTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                     <  2:835480/143‑1 (MQ=255)
                                                                                                               cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                     <  2:228508/143‑1 (MQ=255)
                                                                                                                    aGGCACCGCCGCTCCGCCATGATGTCGCAGGATCAGGTCTTGCTCCGCCCGCTCATTGACGTCGCGGCGTAGAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTCACATAACCCTGCTGTGTAACCAGtt                <  2:427139/143‑1 (MQ=255)
                                                                                                                     ggCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTACCAGCTCTTCGGCACTAACCTCAACCCGCTGCTTAAGCAgcgc               >  2:943240/1‑140 (MQ=255)
                                                                                                                       cAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa             <  1:42099/143‑1 (MQ=255)
                                                                                                                        aGCGACGCACCGCCATGATGGCGCAGGGTCAGGGGTTGGTCCGCCGGGTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat            <  1:398685/143‑1 (MQ=255)
                                                                                                                         gcgcCTCTCCTCCATGATTGCCCAGCATCAGTTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata           <  1:888386/143‑1 (MQ=255)
                                                                                                                          cgccgcACCGCCATGATGGCGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCTGTTTAACCAGTTCGataa          >  2:50182/1‑143 (MQ=255)
                                                                                                                                  cGCCATGATGGCGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg  <  1:312672/143‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                   
TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTG  >  NC_000913/3560314‑3560585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: