Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697–1299500	2–1199	94 [0]	[0] 95	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	89 (1.220)	70/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299365‑1299640                                                                                                                                      |                                                                                                                                               ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                       >  1:1246500‑M1/1‑134 (MQ=255)   agttaTAAGTATTTCCTTAACTGCTTCTCCTCAACATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                     <  2:611540‑M1/143‑12 (MQ=255)   agttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                     <  1:1105869‑M1/143‑12 (MQ=255)     ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  1:1126515‑M1/143‑14 (MQ=255)     ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGGAACGTGCTAAataataatcaatt                                                                                                                                   >  2:697487‑M1/1‑130 (MQ=255)       ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAACAATAATTGAAATGATATTATTAATTCCACTGCCTTTCGTAGAGCAAAGTGCTAActaataatcaattgt                                                                                                                                 >  2:485186‑M1/1‑128 (MQ=255)        taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                >  1:669675‑M1/1‑127 (MQ=255)         aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                                                                                                               <  2:985035‑M1/143‑18 (MQ=255)          aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATTATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                              <  1:1128337‑M1/143‑19 (MQ=255)          aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                              >  1:266771‑M1/1‑125 (MQ=255)           ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTCATTCCACTGCCTTTGGTAGCGGAAAGTCCTAAatactaatcaattgttaag                                                                                                                             >  2:522530‑M1/1‑124 (MQ=255)            gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                                                                                                                            <  1:321388‑M1/143‑21 (MQ=255)             aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                                                                                                                           >  1:1294572‑M1/1‑122 (MQ=255)                     aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                   >  2:705215‑M1/1‑114 (MQ=255)                       cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                                                                                                                 >  1:1251979‑M1/1‑112 (MQ=255)                           ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATAAataataatcaattgttaaattattgagcatttcac                                                                                                             >  2:766118‑M1/1‑108 (MQ=255)                              tccttACCATCATGTTATTTTCTCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                          <  2:195706‑M1/143‑39 (MQ=255)                              tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatagtgcatttcaacac                                                                                                          >  2:249779‑M1/1‑105 (MQ=255)                              tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTACTAAataataatcaatagttaaattattgtgcatttaactaa                                                                                                          >  2:950340‑M1/1‑105 (MQ=255)                                 tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                       >  1:1222940‑M1/1‑102 (MQ=255)                                  cACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAACGTGCTAAataataatcaattgttaaattattgtgcattgcactactgga                                                                                                      >  1:181402‑M1/1‑101 (MQ=255)                                   gccaTCATGTTCTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataataaattgttttattattgtgcatttcactactggaa                                                                                                     <  2:168578‑M1/142‑44 (MQ=255)                                   aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAatcataatcaattgtgaaattattgtgcatttcactactggaa                                                                                                     <  1:936755‑M1/143‑44 (MQ=255)                                   aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     >  2:1054985‑M1/1‑100 (MQ=255)                                   aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  1:451774‑M1/143‑44 (MQ=255)                                   aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     >  1:725975‑M1/1‑100 (MQ=255)                                   aCCATAATGTTATTGTCGGCAAAACATATTGCTGTGCTTGTTGAAGAATAATGGAAATGATATTATTAATTCCACTGGCGTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  2:148993‑M1/143‑44 (MQ=255)                                   aCCAGCAGGTTTTTTTCGGGACTTCATAATCCTGGGCTTGCTGAAGCATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  2:565371‑M1/143‑44 (MQ=255)                                      atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                                  <  1:287909‑M1/143‑47 (MQ=255)                                          tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  2:885562‑M1/143‑51 (MQ=255)                                          tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              >  1:268536‑M1/1‑93 (MQ=255)                                          tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcatttctggaactgtaat                                                                                              >  2:876879‑M1/1‑93 (MQ=255)                                          tGTTATTTTAGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  2:691480‑M1/143‑51 (MQ=255)                                           gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTGGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                             <  2:822343‑M1/143‑52 (MQ=255)                                            ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                            >  1:898874‑M1/1‑91 (MQ=255)                                            ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                            >  1:1187881‑M1/1‑91 (MQ=255)                                                tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                        <  1:198935‑M1/143‑57 (MQ=255)                                                 ttCCCCACATCATAATCCTGGGCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                       <  2:689140‑M1/143‑58 (MQ=255)                                                   cGCCACATCATAATCCTGCGCTTGCTGAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaga                                                                                     <  1:590229‑M1/143‑60 (MQ=255)                                                    gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                    <  1:783511‑M1/143‑61 (MQ=255)                                                     acacatCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                   <  1:908506‑M1/142‑62 (MQ=255)                                                       acaGCATAAGCCTGGGCTTGCGGAAGAATAATTGAAATGATATTATTAATTCCAGTGCGTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                 <  2:474732‑M1/143‑64 (MQ=255)                                                          tcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                              <  1:1257100‑M1/143‑67 (MQ=255)                                                            atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGTCTTTGGTAGAGGATAGTGCTAAataataatcaattgttagattgttgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                            <  2:902675‑M1/143‑69 (MQ=255)                                                            atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaactgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                            >  1:186867‑M1/1‑75 (MQ=255)                                                                    gggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaa                                                                    >  1:517061‑M1/1‑67 (MQ=255)                                                                            tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctttatt                                                            >  1:824870‑M1/1‑59 (MQ=255)                                                                              aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttg                                                          >  1:615567‑M1/1‑57 (MQ=255)                                                                                gaaTAATTGAAATGATATTATTAATTCCACTTCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        <  1:208392‑M1/143‑89 (MQ=255)                                                                                gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcttcttgtaatgggatctgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        >  1:626190‑M1/1‑55 (MQ=255)                                                                                gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        <  1:702068‑M1/143‑89 (MQ=255)                                                                                 aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAgtaataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                       <  1:1111755‑M1/143‑90 (MQ=255)                                                                                    aatTTAAATGATATTATTAAGTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    <  1:370520‑M1/143‑93 (MQ=255)                                                                                    aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAGataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagagatgcttagccaatctctatttgattgag                                                    >  2:399530‑M1/1‑51 (MQ=255)                                                                                    aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    <  2:683813‑M1/143‑93 (MQ=255)                                                                                    aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    >  2:779921‑M1/1‑51 (MQ=255)                                                                                    aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                    <  1:241066‑M1/143‑93 (MQ=255)                                                                                      ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                  >  2:216220‑M1/1‑49 (MQ=255)                                                                                      atggaatgttATTATTAATTCCACTGCCTTTGGTAGAAGGAAGTGCTAAataataatcaattgttaaattattgtgcatttcactaccggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                  <  2:54116‑M1/139‑95 (MQ=255)                                                                                          aaTGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaatttttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                              <  2:301035‑M1/143‑99 (MQ=255)                                                                                               tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagacactctatatattattaaattgaaattttt                                         >  1:1344295‑M1/1‑40 (MQ=255)                                                                                                 ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgaatgaactgaaaaatgttt                                       >  2:1364946‑M1/1‑38 (MQ=255)                                                                                                   attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatcgccacgctaggcgaacggctatgggattgacctgaaagatgtttgt                                     >  2:1321057‑M1/1‑36 (MQ=255)                                                                                                   attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     >  1:1336594‑M1/1‑36 (MQ=255)                                                                                                   attaATTCCACTGCCTTTGGTAGAGGAAAGTGCGAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                     <  1:558192‑M1/143‑108 (MQ=255)                                                                                                    ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                    >  1:750904‑M1/1‑35 (MQ=255)                                                                                                    ttaAGTGCAGTGTCTTTGGTAGAGGAACGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagtattgatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                    <  1:446397‑M1/143‑109 (MQ=255)                                                                                                      aaTTCCACTGCCTTTGGAAGAGGAAAGGGCTAAataataatcaattgttaaattatcgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                  <  1:422990‑M1/143‑111 (MQ=255)                                                                                                       aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 <  1:784037‑M1/143‑112 (MQ=255)                                                                                                       aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 >  2:727954‑M1/1‑32 (MQ=255)                                                                                                          ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                              >  1:204058‑M1/1‑29 (MQ=255)                                                                                                           cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                             >  1:172133‑M1/1‑28 (MQ=255)                                                                                                            aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgcttgttaaggcatg                            >  2:510196‑M1/1‑27 (MQ=255)                                                                                                               gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         <  1:1264217‑M1/143‑120 (MQ=255)                                                                                                               gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         >  1:202578‑M1/1‑24 (MQ=255)                                                                                                                     ggTAGAGGAAAGTGTGAGataataatcaattgttaaagtattgtacatttcactagtggaactgtaatcagaaaagatagatatgcttagccaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                   <  2:510555‑M1/143‑126 (MQ=255)                                                                                                                      gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgggactgtactcagaaaggatagacatgcttagccactctctatgtgattggattggaagatgtttgttcaggcgtgggtggaggct                  >  1:363932‑M1/1‑17 (MQ=255)                                                                                                                      gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                  >  1:1349205‑M1/1‑17 (MQ=255)                                                                                                                       tAGAGGCAAATGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagacaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgccagcga                 >  2:194435‑M1/1‑16 (MQ=255)                                                                                                                        agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                <  2:114572‑M1/143‑129 (MQ=255)                                                                                                                         gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               <  2:903805‑M1/143‑130 (MQ=255)                                                                                                                          agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagcaaagatagacatgcttagccaatctctatttgattgaattgaacgatgtttgttaaggcatggatgcaagctacag              >  2:1045998‑M1/1‑13 (MQ=255)                                                                                                                          agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagaggtttgttaaggcatggatgcaagctatag              >  2:362854‑M1/1‑13 (MQ=255)                                                                                                                              aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  1:705215‑M1/143‑135 (MQ=255)                                                                                                                              aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  1:363163‑M1/143‑135 (MQ=255)                                                                                                                              aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc          <  1:714527‑M1/143‑135 (MQ=255)                                                                                                                               aGTGCTTAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct         <  2:1354924‑M1/143‑136 (MQ=255)                                                                                                                                gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctg        <  1:981771‑M1/143‑137 (MQ=255)                                                                                                                                 tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga       <  2:266771‑M1/143‑138 (MQ=255)                                                                                                                                    tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatac    >  1:217017‑M1/1‑3 (MQ=255)                                                                                                                                    tAAataataatcaattgttaaattattgtgcatttcactactgaaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagctgtttgttaaggcatggatgcaagctatagattctgatac    >  2:73927‑M1/1‑3 (MQ=255)                                                                                                                                     aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg   >  2:518219‑M1/1‑2 (MQ=255)                                                                                                                                     aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcaaggatgcaagctatagattctgatacg   <  2:486206‑M1/143‑142 (MQ=255)                                                                                                                                      aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcccatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagaaatagattctgatccga  >  2:1084559‑M1/1‑1 (MQ=255)                                                                                                                                      |                                                                                                                                               TTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299365‑1299640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑