Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,509,471 G→A intergenic (‑45/‑159) glk ← / → yfeO glucokinase/putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,509,4710GA97.2% 214.1 / ‑6.6 72intergenic (‑45/‑159)glk/yfeOglucokinase/putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (39/31);  minor base C (2/0);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAC  >  NC_000913/2509335‑2509610
                                                                                                                                        |                                                                                                                                           
ttAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATACTTCTTTCTCACACTCTCAAtc                                                                                                                                       >  2:697258/1‑142 (MQ=255)
 tAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAACAATTCTTTCTCACACTATCAATAc                                                                                                                                      >  2:1024284/1‑143 (MQ=255)
  aGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACGTTGTCATTCTTCAACTGCCCCGCTGACGTCAAAATAAGTCTTTCTCACACTATAAATAcc                                                                                                                                     >  1:1307626/1‑143 (MQ=255)
       gCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTgggg                                                                                                                                >  1:1096131/1‑143 (MQ=255)
       gCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTgggg                                                                                                                                <  2:399892/143‑1 (MQ=255)
         gagaTTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGc                                                                                                                              <  1:227947/143‑1 (MQ=255)
               tCACCACTGGCAATATCACACGGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACAATATAACTACCTGGGGGCATagta                                                                                                                        >  1:1040716/1‑140 (MQ=255)
                caccacTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTACTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATGAATACCTGGGGGCATaacaa                                                                                                                       >  1:535337/1‑143 (MQ=255)
                   cacTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaacaacaa                                                                                                                    >  1:1238751/1‑143 (MQ=255)
                    acgggCAATATCGCACAGAGCAAGACGTGCGTTGTTGCCGCCCACCTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaacaacaac                                                                                                                   <  2:334570/140‑1 (MQ=255)
                      tGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTGTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTCTAAATACCTGGGGGCATAACAACAACGc                                                                                                                 >  2:162108/1‑143 (MQ=255)
                           atatCACACAGAGCAAGACGGGCGTTGGTGCCGCCCACATCACCGACTAATGCATGCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGaa                                                                                                            <  1:479311/143‑1 (MQ=255)
                             atCACACAGCGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAag                                                                                                          <  1:569908/143‑1 (MQ=255)
                               cacacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggc                                                                                                        >  1:982145/1‑143 (MQ=255)
                                acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggca                                                                                                       <  1:278138/143‑1 (MQ=255)
                                acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCCAAAGTCAACATACTCCTTTCTCACACTATAAATACCTGGGGGCATAACAACGACTCCGACAaggca                                                                                                       >  2:156349/1‑143 (MQ=255)
                                 cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTACAGTCAAAATAATTCTTTCTCACACTATAAATACCTGCGGGCATAACAACAACGCCGGAAcggcag                                                                                                      >  2:557959/1‑143 (MQ=255)
                                 cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggccg                                                                                                      >  1:906153/1‑141 (MQ=255)
                                      agCAAGACGTGCGTTGGTGCCGCCCACATCACAGACCAAGGCACACTTAGTCATTCTTCAACAGCACCGCTACAGTCAAAATAATTCTTTCTCACACCATAAATACCAGGGCGCATAACAACAACGCCTGAAaggcagcaccc                                                                                                 >  2:482857/1‑138 (MQ=255)
                                        cAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATGAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCt                                                                                               >  2:434393/1‑143 (MQ=255)
                                              gTGCTTTGTTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAat                                                                                         <  1:300092/143‑1 (MQ=255)
                                              gTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAat                                                                                         <  1:300084/143‑1 (MQ=255)
                                                gCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAatat                                                                                       <  1:647651/143‑1 (MQ=255)
                                                gCGTTGGTGCCGACCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAatat                                                                                       <  1:798463/143‑1 (MQ=255)
                                                 cGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATTATTCTTTCTCACACTATAAATCGTTGCAACCAAAACAACATTGTTGGAAACACACGATCCCTGTAAATATc                                                                                      >  1:987199/1‑143 (MQ=255)
                                                  gTTGGGGCCGCCCAGATCACCGACTAATGCAGACTATGTCATTCTGCAACTGCTCCGCTAAAGTCAAAATAATGCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCg                                                                                     <  1:77979/143‑1 (MQ=255)
                                                   ttGGTGCCGCGCACATCACCGACTAATACATACTTTGTCATTCTTCAACTGCTCCGCGGAAGTCAAAAGAGTTCGTCCTCACACTATAAAGACCTGGGGGCATGACAAAAACGCGCGAACGGCAGGCTCTCTGTCAAGAACGa                                                                                    >  2:678458/1‑143 (MQ=255)
                                                   ttGGTGCCGCCCACATCACCGACTAATACAGCAGTGGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGa                                                                                    <  1:1284648/143‑1 (MQ=255)
                                                      cgtccgccCACATCAACGACTAATTCATACCTTGTCATTCTTCTACTGCTCCGCTAAAGGCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCt                                                                                 <  2:1220524/140‑1 (MQ=255)
                                                        gccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg                                                                               <  1:33809/143‑1 (MQ=255)
                                                        gccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCCCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg                                                                               <  1:774415/143‑1 (MQ=255)
                                                          cgccCACATCACCGACTACTGTATGCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATGCTTTCGCACACTATAAATACCTGGGGGCATAACAACAAGGACGGAAAACCAGCCCCCCCGGAAATACCGAGATgggc                                                                             >  2:831798/1‑142 (MQ=255)
                                                           gccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGACAACATAATTCTTTCTCACACTATAAATACCTGGAGGCATAACAACAACGCCGGAAAGGCCTGCGCCCTGCAAACATCCATCTgtgcc                                                                            >  2:1071454/1‑139 (MQ=255)
                                                            cccACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTca                                                                           >  1:937926/1‑143 (MQ=255)
                                                             ccACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTAACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGAACTGGGTcac                                                                          >  1:499217/1‑143 (MQ=255)
                                                                caTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCACAAAAAAACGATCCAGGTcacaca                                                                       >  2:385462/1‑143 (MQ=255)
                                                                 aTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACaa                                                                      <  1:821997/143‑1 (MQ=255)
                                                                  tCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGAGGCATAACAACAACGCCGGAAAGGCAGGCTTCCTGTAAATATCGATCTGGGTCACACAAt                                                                     <  1:261884/143‑1 (MQ=255)
                                                                     ccGACTAATGCATACTTTGTCATTCTTCAACTGCTCTGCTTCACTCAAAATAATTCTTTCTCACACTATAAATCCCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTAc                                                                  <  2:961284/143‑1 (MQ=255)
                                                                     ccGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCGCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTAc                                                                  >  1:874067/1‑143 (MQ=255)
                                                                      cGACTCAGGCATACTTCGTCATTCTTCACCTGCTCCGCTAAAGGCAAAATAATTCTTTCTCGCACTATCAGTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCCATCTGGGTCACACAATTACt                                                                 <  2:670314/143‑1 (MQ=255)
                                                                      cGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt                                                                 <  1:700675/143‑1 (MQ=255)
                                                                       gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCGCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAAAAACCACCTGGGACACACAATTACtt                                                                >  2:674275/1‑143 (MQ=255)
                                                                          tAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTAt                                                             >  1:1088482/1‑143 (MQ=255)
                                                                              gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt                                                         >  2:294063/1‑143 (MQ=255)
                                                                              gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCGCCCTGTAAATATCGATCTGGGTCACCCAATTACTTTATCGtt                                                         >  2:445690/1‑143 (MQ=255)
                                                                              gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCACGCTCCCTGTAAATATCGATCTGGGTCACAAAATTACTGTATCGtt                                                         >  2:444466/1‑143 (MQ=255)
                                                                               cATACTTTGTCATTCTTCAACTGCTCCGCTAACGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGAACTGGGTCAAACAATAACATTATCGgtt                                                        >  2:842826/1‑143 (MQ=255)
                                                                                 tACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCa                                                      <  1:644791/143‑1 (MQ=255)
                                                                                    tttGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCa                                                   <  1:1238257/143‑1 (MQ=255)
                                                                                          aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtt                                             >  1:899268/1‑143 (MQ=255)
                                                                                          aTTCTTCAACTGCTCCGCGAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGACGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCCCCAAtt                                             >  1:442674/1‑143 (MQ=255)
                                                                                             cttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCa                                          >  1:526629/1‑143 (MQ=255)
                                                                                             cttcAACTGCTCCGCTAAAGTCAAAATAACTATTTCACACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCa                                          <  1:1058298/143‑1 (MQ=255)
                                                                                              ttcAACTGCTCCGCTACAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCAACCAATTACTTTATCGTATCACAACCaacatctt                                         >  2:1168021/1‑137 (MQ=255)
                                                                                               tcAACTGCCCCGCTAAAGTCAAAATAATTCTTGCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGc                                        <  1:1068808/143‑1 (MQ=255)
                                                                                                  aCTGCTCCTCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGAt                                     <  2:499033/143‑1 (MQ=255)
                                                                                                        ccGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACACTTACTTTATCGTTTCAGCAGCAATTGCAGCGATGCCttt                               >  1:35963/1‑143 (MQ=255)
                                                                                                         cGCTAAAGTCAAAATAATTCTTTCTCACACTATGAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtttt                              <  1:434393/143‑1 (MQ=255)
                                                                                                                 tCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGCGGCCTTTTTGCAAGCt                      >  1:1350075/1‑143 (MQ=255)
                                                                                                                 tCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGATTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCt                      >  1:115201/1‑143 (MQ=255)
                                                                                                                   aacataatTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg                    <  1:1184479/140‑1 (MQ=255)
                                                                                                                   aaaaTAATGCTTGCGCACACGATAAATACCGGGGGGCAGAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGGTTCAGCAGCAATTGCAGCGATGCCTTTTTGCAAGCTgg                    <  1:9949/143‑1 (MQ=255)
                                                                                                                     aataatTCTTTCTCACACTATAAATACCTTGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGttt                                                        >  1:114752/1‑105 (MQ=255)
                                                                                                                     aataatTCTTTCTCACACTATAAATACCTTGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGttt                                                        <  2:114752/105‑1 (MQ=255)
                                                                                                                      ataatTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCa                 <  2:168266/143‑1 (MQ=255)
                                                                                                                       taatTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAACGGCAGGCTCCCTGTAAATATCCATCTGGGTCACACACTTACTTTATCGTTTCAGCACCAACTGCAGCGATGCCGTCTTGCAGGCTCGGCaa                >  2:916208/1‑143 (MQ=255)
                                                                                                                         atTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCCGCCTCCCTGTAAATATCAATCTGAGTCACACAATTAATTTAACGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAAc              >  1:313442/1‑143 (MQ=255)
                                                                                                                               tctcACACTATACATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAATGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTa        <  1:1260554/143‑1 (MQ=255)
                                                                                                                                 tcACACTATAAATACCTGGGGGCATAATAGAAAAGGGTGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGACGCCTTTTTGCAAGCTGGGCAAACTAAGTATc      >  2:421023/1‑143 (MQ=255)
                                                                                                                                   acacTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTCTCTg    >  1:44203/1‑143 (MQ=255)
                                                                                                                                     acTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCGGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCACTTGCCGCGCCGGCCTGTTGCAGGCGGGGCAAACTAAGTATCTGAc  >  1:249591/1‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAC  >  NC_000913/2509335‑2509610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: