Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,948	A→C	I216S (ATT→AGT)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,948	0	A	C	100.0%	146.5 / NA	49	I216S (ATT→AGT)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/23);  total (26/23)

AAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATAT  >  NC_000913/700809‑701086                                                                                                                                            |                                                                                                                                           aaaGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTgg                                                                                                                                         >  1:657253/1‑143 (MQ=255)        ccGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTc                                                                                                                                  <  1:762865/143‑1 (MQ=255)              tGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGGGTCTTCGCAATc                                                                                                                            >  1:1334277/1‑143 (MQ=255)               ggCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGGTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATcc                                                                                                                           >  1:1148211/1‑143 (MQ=255)               cgCAGCGTTCACCCAGCGGTTCGACCTGACTCTGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATcc                                                                                                                           <  1:332286/142‑1 (MQ=255)                gCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCCATACt                                                                                                                          >  2:436214/1‑143 (MQ=255)                  aGCGTTCACCCAGCGGTTCGACCTGAATACGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGa                                                                                                                        <  1:130538/143‑1 (MQ=255)                   gCGTTCGCCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGAc                                                                                                                       <  2:928272/143‑1 (MQ=255)                   gCGTTCCCCGGGCGGTCCGACCTGAATATGGCCAATTTCACCGACGTTCCCGTTGCGGCCAATAAAAAGGCGCCCGTTAGAGATAATCCCGGCCCCGCTCCCTCGATGGACACGCACCAGACTGGAGGCTTCGCAATCCTGAc                                                                                                                       <  2:1200116/143‑1 (MQ=255)                        cgccccGCGGTTCGACCTGAATATTGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGAGGATGTTAGATATAAGCACGTCGTTGTTGTCGCGATGGACACGAACCAGACTGGAGTCTTCGCAATCCTGACTTGCa                                                                                                                  <  2:288670/141‑1 (MQ=255)                        cACCCAGCGGTTCGACCTGAATATGGCCAGTTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCa                                                                                                                  <  2:23245/143‑1 (MQ=255)                        cACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCa                                                                                                                  <  1:1256879/143‑1 (MQ=255)                                ggTTCGTCCTGAATCTGGCCATTGTCGCCGACGTTGCCGTTGCGGCCAATACAAGTGCGCCCGTTAGAGTTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAagta                                                                                                          <  2:703016/143‑1 (MQ=255)                                ggTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCATACTGGAGTCTTCGCAATCCTGACTTGCACCGAagta                                                                                                          <  1:885246/143‑1 (MQ=255)                                      aCCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGATAGAGATAATCCCGGCCCCGGTTCCGCGATGGAAACGCACCAGACTGGAGTCTTCGCAATCCTGGCTTGCACCGAAAGAGTGccc                                                                                                    >  2:235859/1‑141 (MQ=255)                                                 gCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCcgccagcgcca                                                                                         >  1:851913/1‑143 (MQ=255)                                                 gCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCcgccagcgcca                                                                                         >  1:645355/1‑143 (MQ=255)                                                 gCCAATTTCACCGACGTTGCCGTTGCGGCCAAGAAAAATGCGCCCGTTAGATATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGCAGTAGTGCTCcgccagcgcca                                                                                         >  2:715396/1‑143 (MQ=255)                                                  ccAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCCCCGAAGTAGTGCTCcgccagcgccag                                                                                        >  1:8650/1‑143 (MQ=255)                                                  ccAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCcgccagcgccag                                                                                        >  1:146130/1‑143 (MQ=255)                                                      tttCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTa                                                                                    >  1:1232096/1‑143 (MQ=255)                                                        tCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACg                                                                                  <  1:662148/143‑1 (MQ=255)                                                        cccccGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATTGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACg                                                                                  <  2:993851/140‑1 (MQ=255)                                                                  cgtcgttgcGGCCATTAACACTGCGCCCTTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGa                                                                        <  2:1082022/140‑1 (MQ=255)                                                                               aagaaaaaTGCGCCCGTCAGAGATAATCCCGGCCCCGGTGTCGCGATGGACACGCACCAGACCGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAgg                                                           <  1:363691/140‑1 (MQ=255)                                                                               aaTAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAAGCCGGGCTTGCACAGAAGTAGCGCTCCGCCAGCGCCAGACTACGGAAATCGGGACGAATGAGACCgg                                                           >  2:408367/1‑143 (MQ=255)                                                                                    aaaTGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATTTCGTGACCAACGACACAGGTCACt                                                      >  2:355390/1‑143 (MQ=255)                                                                                          gaccGTTAGAGATAACCCCGGACCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTaaaa                                                <  1:387357/141‑1 (MQ=255)                                                                                           cccGTTAGAGATAATCCCGGCCCCGGTTCCGCGCTGGACACGCACCAGTCTTTTGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCCCTTTAacac                                               >  1:576541/1‑143 (MQ=255)                                                                                                 agagATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGttctt                                         >  1:456444/1‑143 (MQ=255)                                                                                                  gagaTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCGCTTTAAAACGttcttc                                        >  1:437210/1‑143 (MQ=255)                                                                                                   agaTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCCCCAGACTACGGATCTCCTGACGGGCGTAACAGGTCCCTCAAAAACGTTCTTcc                                       >  1:248070/1‑143 (MQ=255)                                                                                                    gatcctCCCGGCCCCGGTTCCGCGACGGACACGCACCAGACTGCGGTCTCCGCAATCCTGACTTGCACCCAAGTAGTGCTCCGCCAGCGCCAGACGACGTATATCGTAACCAACGAAACTGGCCACTTTAACACGTTCTTCCa                                      <  2:580473/138‑1 (MQ=255)                                                                                                     aTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCag                                     >  1:1250477/1‑143 (MQ=255)                                                                                                     aTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCag                                     >  1:1173700/1‑143 (MQ=255)                                                                                                           ccGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCACAGAAAAAGGTAACTTCAAAACGTTCTTCCAGAGCTTc                               >  2:848975/1‑143 (MQ=255)                                                                                                            cGGCCCCGGGTCCGCGATGGACACGCACCAGACTGGAGTCTCCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCt                              <  1:165625/143‑1 (MQ=255)                                                                                                                cccGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTGGGGCGCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTGCTTCCAGAGCTTCTACCa                          >  1:860113/1‑143 (MQ=255)                                                                                                                 ccGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAg                         >  1:888932/1‑143 (MQ=255)                                                                                                                     ttCCGCGATGAGCACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                     <  2:1099464/143‑1 (MQ=255)                                                                                                                     tgccGCGATGGACGCGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCGCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                     <  1:318839/141‑1 (MQ=255)                                                                                                                     ctCCGCGCTGTACACGTACCATACTGGAGTCTTCGCACTCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGCCTACGGATACCTTGACCCACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                     <  2:1057304/142‑1 (MQ=255)                                                                                                                         gcgATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCCGAGCTTCTACCAGCCCCCAGt                 >  1:232412/1‑143 (MQ=255)                                                                                                                          cgATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGtt                <  2:657253/143‑1 (MQ=255)                                                                                                                             tGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTCCGGCTATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCCGCGCTTCTACCACCCCCCAGTtctc             >  1:542937/1‑143 (MQ=255)                                                                                                                               gccccGCACCAGACTGGAGTCTGCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCGTCTACCAGCCCCCAGTTTTCTa           <  2:586883/139‑1 (MQ=255)                                                                                                                                     caccacACTGGAGGCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGaa     <  1:426861/143‑1 (MQ=255)                                                                                                                                      aCCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACGAACCAAAAAAGAGAATGGAGTGCGGTCTTCCAGAGCTTCTACCCGCCCCCAGCTTCGTACCGGAat    >  2:286442/1‑143 (MQ=255)                                                                                                                                        cAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTCCCTGAatat  >  1:880724/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                           AAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATAT  >  NC_000913/700809‑701086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑