breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsEvo04ptsHIcrrEvo01EP_S9_L001_R1_001.good.fq881,999233,677,393100.0%264.9 bases289 bases99.2%
errorsEvo04ptsHIcrrEvo01EP_S9_L001_R2_001.good.fq881,999233,810,646100.0%265.1 bases289 bases96.2%
total1,763,998467,488,039100.0%265.0 bases289 bases97.7%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,652102.55.4100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000011215
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500030
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.004

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.87090

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input05:20:42 27 Feb 202005:21:10 27 Feb 202028 seconds
Read alignment to reference genome05:21:11 27 Feb 202005:28:29 27 Feb 20207 minutes 18 seconds
Preprocessing alignments for candidate junction identification05:28:29 27 Feb 202005:28:59 27 Feb 202030 seconds
Preliminary analysis of coverage distribution05:28:59 27 Feb 202005:30:21 27 Feb 20201 minute 22 seconds
Identifying junction candidates05:30:21 27 Feb 202005:30:53 27 Feb 202032 seconds
Re-alignment to junction candidates05:30:53 27 Feb 202005:32:42 27 Feb 20201 minute 49 seconds
Resolving best read alignments05:32:42 27 Feb 202005:33:31 27 Feb 202049 seconds
Creating BAM files05:33:31 27 Feb 202005:34:41 27 Feb 20201 minute 10 seconds
Tabulating error counts05:34:41 27 Feb 202005:35:26 27 Feb 202045 seconds
Re-calibrating base error rates05:35:26 27 Feb 202005:35:27 27 Feb 20201 second
Examining read alignment evidence05:35:27 27 Feb 202006:05:28 27 Feb 202030 minutes 1 second
Polymorphism statistics06:05:28 27 Feb 202006:05:29 27 Feb 20201 second
Output06:05:29 27 Feb 202006:05:47 27 Feb 202018 seconds
Total 45 minutes 4 seconds