| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,103,656 | Δ1 bp | coding (1320/1374 nt) | cpxA ← | sensory histidine kinase in two‑component regulatory system with CpxR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,103,654 | 0 | G | . | 98.2% | 242.9 / ‑3.3 | 56 | coding (1322/1374 nt) | cpxA | sensory histidine kinase in two‑component regulatory system with CpxR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (25/30); minor base C (1/0); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA > NC_000913/4103512‑4103791 | ccTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTCTACAGCGGCAACCAAATCACCAGCCCTCACCCGCCCAGCg > 2:843374/1‑143 (MQ=255) gATAAGACGCTATCAGCGTCGCATCAGGTATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggct < 1:452863/143‑3 (MQ=255) aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCcgcgcctg > 1:900505/1‑139 (MQ=255) aGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGtct < 2:19217/143‑1 (MQ=255) aCGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTAAAGTCAAGTTCGAAGTTTAACCCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGtcttc < 1:1092917/143‑1 (MQ=37) tcTATCTGCGTCGCTTCAGGCATCCTGCCCAAGTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCAAATGCGAAGCTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTg < 2:643467/142‑1 (MQ=37) tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGCTGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAACTCACCCGCCGTACACCGCCCCGC‑GGCCGCCTTCTGcc > 1:301015/1‑143 (MQ=37) tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGcc > 2:543494/1‑143 (MQ=255) tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGcc < 2:516349/143‑1 (MQ=255) aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCt > 1:178804/1‑143 (MQ=255) tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCtt > 1:1098557/1‑143 (MQ=255) tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCtt < 1:18558/143‑1 (MQ=255) gTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCcccc > 1:883540/1‑143 (MQ=255) gTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCAccc < 2:1004822/143‑1 (MQ=255) gCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGc < 1:861168/143‑1 (MQ=255) aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCCGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGa > 1:79585/1‑143 (MQ=255) aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGa < 1:610847/143‑1 (MQ=255) aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGa < 1:71808/143‑1 (MQ=255) gCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATCCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTCCTGCCTTCACCCAGCCACGAtg > 2:839811/1‑143 (MQ=255) cTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATgctgct < 1:835848/143‑1 (MQ=255) tGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGctgctg > 1:1026463/1‑143 (MQ=255) gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGa > 1:82700/1‑143 (MQ=255) gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGa < 1:845438/143‑1 (MQ=255) gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCACTTATTCAGCGACAACCAAATCACCAGACGTAAAATCATCA‑CCGGCTATCATCTGCCTTCACCACGCCACTATACTGCTGa > 2:650688/1‑143 (MQ=21) cAAACGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATg < 1:978756/143‑1 (MQ=255) aaTGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGc > 2:306943/1‑143 (MQ=255) cGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTACCTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGttt < 2:346389/143‑1 (MQ=255) cGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATGCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGttt < 2:299062/143‑1 (MQ=255) atccggcgTCACCGCCTTATCCCGCCTGCAAATGCGACGTTTACCCCCGCTTCTACAGCGGCAACCAACTCCCCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTcaa < 1:62112/140‑1 (MQ=255) aTGCGGCGTAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTCAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTcaa < 1:1002549/143‑1 (MQ=255) ggcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCCGCCACGATGCTGCTGAATGGCGGTTTCAACAAt > 1:598113/1‑143 (MQ=255) gcgTAAACGCCTTATCCTGCCTGCAACTGCGAAGTTTAACTCCGCTTATACAGCGGCCACCAAATCACAAGCCGTAAACCGCCCCGC‑GGCGGTCTGCTGCCATCACCCAGCAGCGATGCTGCTGACTGCCGGTGTCAACAATc > 2:441961/1‑143 (MQ=255) gcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATc < 2:96820/143‑1 (MQ=255) gcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATc < 1:306943/143‑1 (MQ=255) acgctgcctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAAc < 2:797310/140‑1 (MQ=255) aTCCTGCCTGCAAATTCGACGTTGAACTCCGCTTACACAGCGGCAACCAAATCGCCAGCCGTAAACCGCCCCGC‑GGCTGTCTTCTGCCTTCACCCCGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAAc < 2:805573/143‑1 (MQ=255) tCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAGcc > 1:641141/1‑143 (MQ=255) aaaTGCGAAGGTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCa < 1:774020/143‑1 (MQ=255) tAACTCCGCTTATACAGCGGCAACCAAATCACCACCCGTACACCGCCCAGC‑GCCCCTCTTCTGCCTTCACCCACCCACGATGCTGCTGAATGGCGGTCTTACCCATCGCCACCCCCAACCCTCTCACGCCAGACCCACCATcg > 2:392879/1‑143 (MQ=255) tCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATcgcggt > 1:368023/1‑141 (MQ=255) tCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACCACcgcgca > 2:562834/1‑141 (MQ=255) cGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCt < 1:285574/143‑1 (MQ=255) cGCTTAGACAGCGGCGCGCGAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTGCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCt < 1:843374/143‑1 (MQ=255) aGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTa > 1:817542/1‑143 (MQ=255) aGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCCGAATGGCGGTTTCAACAATCCCCAGCCCCACACCAGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTa < 1:220300/143‑1 (MQ=255) ggCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGa < 1:277390/143‑1 (MQ=255) gCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTAGCGCCAGCTTCACGATCGCGTGCTCCATCGGCCCGAt > 2:508482/1‑143 (MQ=255) aaCCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCCGTCTCAACAATCGCCAGCCCCAAAGCTGGACCGCCAGACTCACGATCGCGTCCTCCCTCGGCACGCTac > 2:159438/1‑142 (MQ=255) aaaTCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacg < 1:1035624/143‑1 (MQ=255) aaTCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacgg > 1:985418/1‑143 (MQ=255) aaTCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATACTGCTCGATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGACAGATTCACGATCGCACGCTTCCTCGGTACGATAGAacgc > 2:130684/1‑142 (MQ=255) cGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATc < 1:1040193/143‑1 (MQ=255) aaaCCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGt < 2:631227/143‑1 (MQ=255) aCCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGCGGGAGAATCGGtgc > 1:675791/1‑141 (MQ=255) ccGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcg > 1:1005995/1‑143 (MQ=255) cGCCCAGC‑GGCTGTCCTCTGCCCCCACCCAGCCACGATGCTGCTGAATGGCTGTTTCACCGACCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcgc < 1:405697/143‑1 (MQ=255) cccAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGa < 1:439668/143‑1 (MQ=255) cccAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGa > 1:644420/1‑143 (MQ=255) cccAGC‑GGCTGGCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTGCCACAATCGCCAGCCCCAATCCTGTACCGCCAGCTTAACGATCGTGATTGTCATCGGTTCGATAGCACGGGCGGAGCATCTGTGcgcgg > 1:40687/1‑142 (MQ=255) | CCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA > NC_000913/4103512‑4103791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |