| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 96.0% | 210.0 / 0.9 | 50 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (23/25); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 96.0% | 207.9 / 0.1 | 50 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (23/25); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAA > NC_000913/2173221‑2173488 || aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑Taccaccg > 1:1077531/1‑142 (MQ=255) aCGATGCCCGGCGCCCGCCATCCCCACGAAGACCCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑Taccaccgccgtat < 1:81663/143‑8 (MQ=255) cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGACAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCca < 2:500186/143‑1 (MQ=255) aCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCcag > 1:197764/1‑143 (MQ=255) aCGGCGACCGCCAGCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCGATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCcag < 1:875501/143‑1 (MQ=255) ccGCCATCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAAc < 1:781196/143‑1 (MQ=255) ccATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAACAAACCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc > 1:628005/1‑143 (MQ=255) aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCGGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCgg > 1:474484/1‑143 (MQ=255) tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa < 2:165198/143‑1 (MQ=255) cGCCAAGAAGAAGCCGCGGGTGGCAAGAGGGCCAAACCGCAGCACCGGATTACCCGGCAAACAAACAGCGATTAAAAT‑‑GGTGAGTG‑GGATAAGAATCAGGCTTGGCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt < 1:217345/143‑1 (MQ=255) cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCaa > 1:1036709/1‑143 (MQ=255) cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCaa > 1:513359/1‑143 (MQ=255) aCGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 1:232257/143‑1 (MQ=255) cgacgaAGCCGATGGTGGCAAGATCGCCAAACGGTACCACCTTTTTACCCGGCACACAAACAG‑‑ATCAACTTTAGGTGACTT‑CGCTAAAAAACA‑TTTCGTTGAGTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg < 1:803918/143‑1 (MQ=255) ccgCCGATGGTGGCAAGCTCGCCAAACGGCAGCACGCGACTACTCGGCCCACAAACAGCACTCACAAA‑‑GCCGAGTGTTGCTCTGGA‑CATCCTGGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCTGATCAAGGCCAAt < 2:231348/141‑1 (MQ=255) agCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc > 1:437731/1‑143 (MQ=255) agCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc < 1:483384/143‑1 (MQ=255) gcagATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTAGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa > 2:1022375/4‑143 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGTCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg > 1:227897/1‑143 (MQ=255) gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTACCAGCAGCAACGCCGGACCAAGGCCGATCAGGa > 2:462716/1‑143 (MQ=255) aTGGTGTCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa < 1:678412/143‑1 (MQ=255) caggCAAGAACCCGAAACGGCAGCACCCGAGTACCCGGCACACAAACAGCAAGTAAAAT‑‑GGTGAGTA‑GGACAAAAATCAGGCTTGCCGA‑TAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc < 1:121391/141‑1 (MQ=255) tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc > 1:108372/1‑143 (MQ=255) gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTg > 2:126685/1‑143 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc > 1:1031436/1‑143 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGCGAGTG‑GGATAACAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACACCGGACCAAGGCCAATCAGGACCCCCTGAcccg > 2:299370/1‑141 (MQ=255) tCGCCAAACGGCAGCACCTGATTACCCAGCACACAAACAGCAACTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc < 1:404088/143‑1 (MQ=255) gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGa > 1:521497/1‑143 (MQ=255) aaaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAGCAGGAACTCCTGACCGCCGCACt > 2:218561/1‑143 (MQ=255) ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 2:252335/143‑1 (MQ=255) ggCAGCACCTCATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 1:483269/143‑1 (MQ=255) gcagcaCCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGGGGGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc < 2:619903/143‑1 (MQ=255) caCCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGCCTTGCCGA‑AACCA‑‑CCGCCGGATGTCCCAGCAGCAACGCCGGATCAAGGCCAGTCAGGCACTCCGGACGGCCGCGCTTCGccccca > 2:1098757/1‑139 (MQ=255) cTGATGTCCCGGCACATCAACAGCAATGGAAGT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACTGCCGAACTTCGCCTGTAAAc < 2:742593/143‑1 (MQ=255) ccGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa < 1:778335/143‑1 (MQ=255) aaaCAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTa < 1:1054222/143‑1 (MQ=255) aCAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCGGTAAACGACTACGCGCGTGCTTAGc > 1:932381/1‑143 (MQ=255) aCAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCTGCTTAGc > 2:651961/1‑143 (MQ=255) aGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTCCGCCTGTACACGACTACGCCCCTGCTTAGCGa > 2:664980/1‑143 (MQ=255) aGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTAAGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACCTCACCTGTAAACGACGCCGAGCCTCCTACGCGa > 2:1005202/1‑143 (MQ=255) cAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGCCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACTACGAGCCTACGTCGCGATg > 2:208566/1‑143 (MQ=255) tAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg < 2:203143/143‑1 (MQ=255) aaaT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TTCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACCTCGCCTGTAAACGACTACGAGCCTGCTCAGCGATGGGCGtt > 2:488033/1‑143 (MQ=255) aT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCATGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaa < 1:777019/143‑1 (MQ=255) aT‑‑GGTGAGGG‑GGATAAAAATCGGGCTTGCCGC‑GAC‑‑CCCCGCCGTGCGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaa < 1:851089/143‑1 (MQ=255) t‑‑GGTCAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa < 1:629830/143‑1 (MQ=255) aGTG‑TGACAACAATCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCCATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc < 1:98842/143‑1 (MQ=255) gg‑ggAGAAACATGACGCTGGCCGA‑TGCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTGCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcca < 1:384638/142‑1 (MQ=255) gATAAAAATCAGGCGTGCCGA‑CACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga < 2:692423/143‑1 (MQ=255) aaacaTCAGCCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCACCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg < 1:23305/143‑1 (MQ=255) tCAGGCTTGCCGA‑TACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTCGACGACTACGAGCCTGGTTAGCGATGGGCGGTCAGCCATCCATGCTGGGTTTa > 1:435763/1‑143 (MQ=255) cAGGCTTGCCGC‑TACCA‑‑CCTCCGTATGTCCCAGCAGCAACGCCTGATCAAGGCCAATCAGGAACTCCTGCCCGTCGTAGTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 1:153409/143‑1 (MQ=255) || ATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAT‑‑GGTGAGTG‑GGATAAAAATCAGGCTTGCCGA‑TACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAA > NC_000913/2173221‑2173488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |