Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,316,760	T→C	V195A (GTC→GCC)	rcsB →	response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,316,760	0	T	C	100.0%	175.0 / NA	58	V195A (GTC→GCC)	rcsB	response regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/24);  total (34/24)

GCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAA  >  NC_000913/2316623‑2316899                                                                                                                                          |                                                                                                                                            gCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCgaga                                                                                                                                        >  1:535765/1‑143 (MQ=255)       ctcGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCCGTATTAAAACAATCAGTAGCCAGAAGAACTCTGCGATGATGAAGCTGGGAGCCGAGGACGatc                                                                                                                                  >  2:977381/1‑142 (MQ=255)          gCCAAAAGGGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCg                                                                                                                               <  2:564438/143‑1 (MQ=255)          gCCAAAAGAGAGCGAAGTTCTGCGCCTGTTTGCGGAACGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCg                                                                                                                               <  1:667028/143‑1 (MQ=255)          cccAAAAGAGAGTGACGCTCTGCCCCTGCTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTTAACCGCAGCATTAAAACCAGCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCg                                                                                                                               <  1:584194/142‑1 (MQ=255)               aaGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAGCGATATCGGCctg                                                                                                                          >  1:495883/1‑143 (MQ=255)                agagagTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAACACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCctgc                                                                                                                         >  1:448138/1‑143 (MQ=255)                   gagTGAAGTTCTGCGCCTGTTTGCGGAACGCTTCCTGGTGACCGAGATCACTAAAAAGCTGAACCGCAGTAGTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGa                                                                                                                      <  1:203412/143‑1 (MQ=255)                    agTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCGGAACAGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGCGCCCGAGAACGATAACGCCCTGCTCaa                                                                                                                     >  2:232068/1‑143 (MQ=255)                          gtccTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTAtct                                                                                                               <  1:344138/140‑1 (MQ=255)                          gTTCTGCGCCTGGTTGCGGGAGGCTGCCTGGTGACCGAGATCGCTAAAACGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAGGACATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTAtct                                                                                                               <  2:90127/143‑1 (MQ=255)                             cTGCGCCTGTTTGCGGATGGTTTTCTGGTTGCCGATTTCGCTAAAACGCTGAAGCGCAGTATTAAAACCGTCAGTAGCCAGAATAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTAtctctc                                                                                                            <  2:679460/143‑1 (MQ=255)                              tGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATctctct                                                                                                           >  1:484769/1‑143 (MQ=255)                              tGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCCCAGTACTAAAACCATCAGGCGCCAGAAGACATCTGCGATGATGAAGCTGGGCGCCGCGACCGACATCGCCCTGCTGACTCAtctctcc                                                                                                           >  2:71600/1‑142 (MQ=255)                               gcgcCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCACTGCTGAATTATCTCCCtt                                                                                                          >  2:583625/1‑143 (MQ=255)                               gcgcCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTAGTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATAGCGCCCTGCTGAAGTACCCCTCtt                                                                                                          >  2:268291/1‑143 (MQ=255)                                   cTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATGATCTCTCTTCAGt                                                                                                      >  1:820373/1‑143 (MQ=255)                                    tGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAACTCTGCGATGATGACGCTAGGTGCCGAGCACGATAGCGCCCTGCTCAACTATCTCTCTTCAGTg                                                                                                     >  2:8344/1‑143 (MQ=255)                                    tGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGCATTGTCTCTCTTCAGTg                                                                                                     >  1:91988/1‑143 (MQ=255)                                    tGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTg                                                                                                     >  1:300626/1‑143 (MQ=255)                                         gCGGAACGCTTCCTGGGGACCGAGAGCGCTAAAGAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACCATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCtt                                                                                                <  2:1077391/143‑1 (MQ=255)                                           ggAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAACACCATCAGTAGCCAGAAGCAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGCATTCGCTCTCTTCAGTGCCCTTac                                                                                              >  2:805612/1‑142 (MQ=255)                                                 cTTTCTGGTAAACGAGATCGCTAAAAAGCTGAATCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCGGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg                                                                                        <  1:263026/143‑1 (MQ=255)                                                  ttCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGTTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc                                                                                       <  1:800464/143‑1 (MQ=255)                                                  ttCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc                                                                                       >  2:428395/1‑143 (MQ=255)                                                   tCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGACGAAATCGGCGATGATGAAGCTGGGCGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCGTCAGAGACCTTAACTCCGGCa                                                                                      >  2:71254/1‑143 (MQ=255)                                                      tGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTCATAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGACCTGCTGAATTATCTCTCTTCCGTGACCTTACGTCCGGCAGAt                                                                                   >  2:638184/1‑143 (MQ=255)                                                        gTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATaa                                                                                 <  2:865805/143‑1 (MQ=255)                                                         tGACCGAGATCGCTGAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGCAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATaaa                                                                                <  1:39295/143‑1 (MQ=255)                                                          gACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAg                                                                               <  1:773198/143‑1 (MQ=255)                                                           actgagaTCGCTAAAAAGCTGAACCGCAGTAGTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGa                                                                              <  1:216055/140‑1 (MQ=255)                                                            ccGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGAc                                                                             <  1:353159/143‑1 (MQ=255)                                                             cGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGCCCTTAAGTCCGGCAGATACAGCCt                                                                            >  1:452092/1‑143 (MQ=255)                                                               agaACGCGAGAAAGCTGAACCGCAGTATTAAAACCATCCGTAGCCAGAAGAAATCTGCGATGCTGACGCTCTCTCCCCAGACCGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTaa                                                                          <  1:101493/143‑1 (MQ=255)                                                                    gCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCACTGCTGTATTATGTCTCTTCAGTGACTTCAAGTTGGGCAGATACAAATTAaccacc                                                                     >  2:62250/1‑143 (MQ=255)                                                                     cTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCt                                                                    >  2:1033963/1‑143 (MQ=255)                                                                      tAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATTTCTCCTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTg                                                                   >  1:245559/1‑143 (MQ=255)                                                                      tAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAGGACTAATCACCTg                                                                   >  1:442274/1‑143 (MQ=255)                                                                      tAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTg                                                                   >  1:1091079/1‑143 (MQ=255)                                                                                aaCCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCGCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGAt                                                         <  1:904460/143‑1 (MQ=255)                                                                                aaCCGAAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGAGGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTGTCTTCAGTGCCCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGAt                                                         <  2:212888/143‑1 (MQ=255)                                                                                  ccGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGAtca                                                       >  2:186712/1‑141 (MQ=255)                                                                                   cGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGCAGTATCTCTCTTCAGTGACCATAAGTCCGGCAGATTAAGCATAATCACCAGTAGcccctgtaag                                                      >  2:732795/1‑136 (MQ=255)                                                                                   cGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTACGTCCGGCAGATACAGACTAATCACCTGAAGGCCAGATaaa                                                      >  1:624850/1‑142 (MQ=255)                                                                                             aaaCCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTTACATTAAAACCGTCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAgt                                            >  1:977358/1‑143 (MQ=255)                                                                                             aaaCCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCGTAAGTCCGGCAGATCAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAgt                                            >  2:399614/1‑143 (MQ=255)                                                                                               aCCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAgtgt                                          <  1:857837/143‑1 (MQ=255)                                                                                                 cATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGCCt                                        >  2:638878/1‑143 (MQ=255)                                                                                                     cgTAGCCCGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATc                                    <  1:169589/142‑1 (MQ=255)                                                                                                       tAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTg                                  <  1:394694/143‑1 (MQ=255)                                                                                                             gaagaaATCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTCTCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGGCTTATCTGGCAttt                            >  1:889585/1‑143 (MQ=255)                                                                                                                 aaaTCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCCGTGACCTTACGTCCGGCAGCTCAACACTCATCCCCTCTCGCCCAGACAAGCCCCGCTAGTCTCCTACCTGCCactcaccc                        >  2:430306/1‑136 (MQ=255)                                                                                                                  aaTCTGCGATGATGAAGCTGGGTGCCGAGTACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCAcc                       <  2:107474/143‑1 (MQ=255)                                                                                                                   aTCTGCGATGATGAAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg                      <  1:60223/143‑1 (MQ=255)                                                                                                                    tCTGCGATGATGAAGCTGGGTGCCGAGAACGATAGCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGa                     <  1:758407/143‑1 (MQ=255)                                                                                                                            gatgaAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTCGTGTCTTGTCTGGCATTTGCACCGATTGCCGGa             >  1:331281/1‑143 (MQ=255)                                                                                                                               gaAGCTGGGTGCCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATgc          >  1:626771/1‑143 (MQ=255)                                                                                                                               gaAGCTGGGTGCCGAGAACGATATCACCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCCCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATgc          >  2:82870/1‑143 (MQ=255)                                                                                                                                       gtgtCGAGAATGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTaaa  <  2:48461/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            GCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAA  >  NC_000913/2316623‑2316899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑