Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,104	G→A	A274V (GCA→GTA)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,104	0	G	A	100.0%	150.4 / NA	47	A274V (GCA→GTA)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/22);  total (25/22)

TTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGC  >  NC_000913/3307966‑3308241                                                                                                                                           |                                                                                                                                           ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCCGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTa                                                                                                                                        >  2:45767/1‑143 (MQ=255)    aCGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATAACGGGATGATGTGTACGTCAGAGTAGCCGGGGCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCg                                                                                                                                     >  1:1093101/1‑143 (MQ=255)                  ccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGt                                                                                                                       >  2:852710/1‑143 (MQ=255)                  ccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGCCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGt                                                                                                                       >  2:768158/1‑143 (MQ=255)                     cACCCGTTAAGGTGATGGCAATCACAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTAt                                                                                                                    <  2:417177/143‑1 (MQ=255)                             gggggtgtGGCAATCAGAAGAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACgttg                                                                                                            <  2:316102/136‑1 (MQ=255)                               ggTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                                                                                          <  1:605424/143‑1 (MQ=255)                                      gCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCaagc                                                                                                   >  1:159309/1‑141 (MQ=255)                                         aTCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                                                                                                <  1:732194/143‑1 (MQ=255)                                          ttaaaaaaGATTACGGGCTGATGTATACGTCAGCTATTGCTGGTCCGATTCTGCCAGTTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCa                                                                                               <  2:893749/141‑1 (MQ=255)                                              aaaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGttt                                                                                           >  1:595602/1‑143 (MQ=255)                                                       cGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGc                                                                                  <  2:1078999/143‑1 (MQ=255)                                                           cTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTg                                                                              <  1:836243/143‑1 (MQ=255)                                                             gATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCATCAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgc                                                                            <  2:636695/143‑1 (MQ=255)                                                             gATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCCCAACAAAGTTATGAACGATGTTGGCAACCGCCAGTCTCAACAGTGTCATGgc                                                                            >  2:169187/1‑143 (MQ=255)                                                              aTGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCCTCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGGAACCCCCAGTTTGAACCGTGCCGTGgcg                                                                           >  1:957034/1‑143 (MQ=255)                                                              aTGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTTGCAACCGCCAGTTTGAACCGCGCCGTgggg                                                                           >  1:111382/1‑141 (MQ=255)                                                                   tACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTc                                                                      >  1:490360/1‑143 (MQ=255)                                                                            tcttGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATcccc                                                             <  1:496717/141‑1 (MQ=255)                                                                            tATTGCTGGTCCGATTCTGCCAGATCATCTTGGTCCTGGCCCAGGAGAGATAATACCAACAATAAATAACGGTGCTCCAAAAAGTTATGAACGGTTTGAGCAACCGCCAGCTGAAACAGTGAGGCAGCGCTGGCAAAAAccca                                                             >  2:289713/1‑142 (MQ=255)                                                                               tGCCGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTAAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAg                                                          <  1:185214/143‑1 (MQ=255)                                                                                 cTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGAc                                                        >  1:898391/1‑143 (MQ=255)                                                                                 cTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCAGTGCTCAACAAAGTAATGAACGTTGTTGGCAACCGCCAGGTTGAACAGTGCCGTGGCGCTGGCCAAGTCCCCCAgcc                                                        >  1:956705/1‑141 (MQ=255)                                                                                  tGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTGGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACt                                                       >  1:93551/1‑143 (MQ=255)                                                                                     tCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACACAGTTATGAACGTTGTTGGAAAACGACAGAGTAAACAATGCTTGAGCGATGTCAAAATCCCCCAGACTGAg                                                    >  2:177039/1‑143 (MQ=255)                                                                                     tCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    <  2:1093101/143‑1 (MQ=255)                                                                                     tCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    >  2:136550/1‑143 (MQ=255)                                                                                     tCCGACTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    <  2:982555/143‑1 (MQ=255)                                                                                     cccGCTTCTGCCCGGTCATCTTGGTCCTGTCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    <  1:1020322/142‑1 (MQ=255)                                                                                        gATGCTGCCAGGTCATCTTGGGCCGGGCCCAGGAGGGATCCGTCGAACAGTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGgt‑a                                                <  2:227562/143‑1 (MQ=255)                                                                                          ttCTGCCAGGTCATCTTGGTCCTGGCCAAGGAACGATAATTGCAACAATACGTATCGGTGCTCAACAAATTTATGAACGTTGTTGGCAACCGCCAGTTTGAAAAGTGACTGGGCGATGTCCAAATGCGCCAGACTTAGgt‑agt                                              >  2:455068/1‑143 (MQ=255)                                                                                           tCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGgt‑agta                                             <  1:67345/143‑1 (MQ=255)                                                                                                cAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTAc                                        >  1:544924/1‑143 (MQ=255)                                                                                                cAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGCTTCAACAGTGTTGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACttgc                                        >  1:203612/1‑141 (MQ=255)                                                                                                cAGGTCATCGTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAAGTACtac                                         <  2:577695/143‑3 (MQ=255)                                                                                                 aGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTTTTGGCAACCGCCAGTTGAAACAGTGCGGGGGCGCGTTGCACATCGCACAGCGTTCGGT‑AGTACtgccc                                       >  2:876294/1‑139 (MQ=255)                                                                                                  gggcATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTAGCGGTGCTCAACAAAGGAATGAACGTGGTTGGCAACCGGCGGGTTGAACAGTGCCGTGGCGCTGTCCAACTCCCCCAGCCCTAGGT‑AGtacctaccg                                      >  2:469960/4‑142 (MQ=255)                                                                                                   gTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTa                                     >  1:330529/1‑143 (MQ=255)                                                                                                               cTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTgg                         <  1:358592/143‑1 (MQ=255)                                                                                                                    ccAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTATTGGCAACCGCCCGTTTGCACAGTGCCGTGGCGCTGCCCAAATCCCCCCGACTTAGGT‑AGTACTTACCTGAATCGATGTAGGTTTcc                    >  2:102007/1‑142 (MQ=255)                                                                                                                      aGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACt                  <  1:852710/143‑1 (MQ=255)                                                                                                                         aGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTgag               <  1:363670/143‑1 (MQ=255)                                                                                                                                  tCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGTACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagc      <  1:750164/143‑1 (MQ=255)                                                                                                                                    caacaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcga    >  1:259515/1‑143 (MQ=255)                                                                                                                                    caacaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACATAAATAGAAGTTGGTTTCACTGAGATGCTCagcga    >  1:422909/1‑143 (MQ=255)                                                                                                                                     aacaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcgag   <  1:66320/143‑1 (MQ=255)                                                                                                                                      acaaTACGTATCGGTGCTAAACAAAGTTATGAACGTTGTGGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTcagccagc  <  2:1023632/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           TTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGC  >  NC_000913/3307966‑3308241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑