Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	45 [0]	[0] 45	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	43 (1.020)	35/276	0.2	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299363‑1299640                                                                                                                                        |                                                                                                                                               agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                         <  1:771632‑M1/143‑8 (MQ=255)   ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGATGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                       >  2:634193‑M1/1‑134 (MQ=255)       ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  1:217502‑M1/143‑14 (MQ=255)       ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  2:446824‑M1/143‑14 (MQ=255)       ttaTAAGGATTTCCTGAACTGCTTCGCCGCACCATCATGTTATTTTCGCCACATCATAAGCCTGGGCTTGCTGAAGAATAATTGAAATGATATTAGTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  1:68654‑M1/143‑14 (MQ=255)              gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                                                                                                                            >  1:327955‑M1/1‑123 (MQ=255)                   ccTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattg                                                                                                                       <  2:76790‑M1/143‑26 (MQ=255)                     ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                     >  2:140877‑M1/1‑116 (MQ=255)                          tGCTTCTCCTCACCATCATGGTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAAGGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                <  2:128027‑M1/143‑33 (MQ=255)                            cTTCTCCTCACCGTCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                              <  2:218275‑M1/143‑35 (MQ=255)                            cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                              <  1:338400‑M1/143‑35 (MQ=255)                             ttCTCCTGACCATCATGTTGTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                                                                                                             <  2:562250‑M1/143‑36 (MQ=255)                             ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                                                                                                             <  1:763700‑M1/143‑36 (MQ=255)                               ctcctcTCCATGTGGTAACTTTCTCCAGATCATAATTCTGGGCTTGCTGAAGAAGAATTGAAATGATATTATTAATTTCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                           <  2:507627‑M1/143‑38 (MQ=255)                               ctcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                           <  2:566205‑M1/143‑38 (MQ=255)                                tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGACATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                          >  1:704613‑M1/1‑105 (MQ=255)                                 cctcGCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                         <  2:342648‑M1/143‑40 (MQ=255)                                     aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     >  2:743769‑M1/1‑100 (MQ=255)                                       catcgtGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                   <  1:412485‑M1/143‑46 (MQ=255)                                              ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATCTTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttacattatagtgcatttcactactggacctgtaatca                                                                                            >  2:443425‑M1/1‑91 (MQ=255)                                                   ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCATTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                       >  1:495662‑M1/1‑86 (MQ=255)                                                       ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATACAACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgaaactgtaatcagaagagata                                                                                   >  1:646606‑M1/1‑82 (MQ=255)                                                        cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                  <  1:230769‑M1/143‑63 (MQ=255)                                                        cacaTCATAATCCTGGGCTTGCTCAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                  <  1:67120‑M1/143‑63 (MQ=255)                                                         acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                 >  1:254049‑M1/1‑80 (MQ=255)                                                                   ccTGGGCTTGCTGAAGAGTAATTGAAATGATATTATTGATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                       <  2:643643‑M1/143‑74 (MQ=255)                                                                            gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctcta                                                              <  2:100187‑M1/143‑83 (MQ=255)                                                                              tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                            >  2:634433‑M1/1‑59 (MQ=255)                                                                                 agaaTAATTGAAATGATATTAGTAATTCCACTGTCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                         <  2:137966‑M1/143‑88 (MQ=255)                                                                                  gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                        <  1:552071‑M1/143‑89 (MQ=255)                                                                                   aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                       <  1:427685‑M1/143‑90 (MQ=255)                                                                                            aaTGATATTATTAAGTCCACTGCCGTTGGTAGAGGAAAGTGCTTGtttataatcaattgttaaattatggtgcatttcactactggaactgtaatcagaaaagagagacatgcttagccaatctctatttgattgaattgaaa                                              <  1:532310‑M1/143‑99 (MQ=255)                                                                                                         aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaacgatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 >  2:127646‑M1/1‑32 (MQ=255)                                                                                                              aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatg                            <  1:594506‑M1/143‑117 (MQ=255)                                                                                                              aCTGCATTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgttcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatg                            >  1:662880‑M1/1‑27 (MQ=255)                                                                                                                      tGGTAGAGGAAAGTGCTAAataataatcaattgttaaataatggtgcattacactactggaactgtaatcagaaaagatagacatgcttagccaatctctatatgatagaatagaaagaagttagataagccacgaatgcaag                    >  2:546386‑M1/1‑19 (MQ=255)                                                                                                                       gttagAGGAAAGTTATAAataataatcaattgataaattattgtgcatttcactactggaactgtaatcagaaaacatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                   <  2:719798‑M1/141‑126 (MQ=255)                                                                                                                         tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta                 >  1:540968‑M1/1‑16 (MQ=255)                                                                                                                           gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               >  1:686019‑M1/1‑14 (MQ=255)                                                                                                                           gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               >  1:669861‑M1/1‑14 (MQ=255)                                                                                                                           gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               <  1:10606‑M1/143‑130 (MQ=255)                                                                                                                            agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatag              >  1:366773‑M1/1‑13 (MQ=255)                                                                                                                            agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacaggcttagccaatcctaatattatttatttgaaagatgtttgttaaggcatggatgcaagctatag              >  1:743508‑M1/1‑13 (MQ=255)                                                                                                                                       aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg   >  2:100793‑M1/1‑2 (MQ=255)                                                                                                                                        aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacgg  <  2:254049‑M1/143‑143 (MQ=255)                                                                                                                                        |                                                                                                                                               AGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299363‑1299640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑