Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,410	A→G	V20A (GTG→GCG)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,410	0	A	G	100.0%	135.0 / NA	48	V20A (GTG→GCG)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

TACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTT  >  NC_000913/754273‑754549                                                                                                                                          |                                                                                                                                            tACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCAAGCGTGCATCTCAGcgcaccg                                                                                                                                        >  2:583935/1‑140 (MQ=255)     ttCATCACCATCAATAAAAGTAATTTTCGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGTTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCACCGTGCCTTTCAGCGCATTCAGta                                                                                                                                    >  2:367828/1‑142 (MQ=255)     ttCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGtt                                                                                                                                    <  1:178956/143‑1 (MQ=255)         tcaCCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCCAc                                                                                                                                >  1:421394/1‑143 (MQ=255)          caCCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCCGCGTGCCTTTCAGCGCATCCCGTTCCAcc                                                                                                                               >  1:776490/1‑142 (MQ=255)          caCCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACa                                                                                                                               >  2:496951/1‑143 (MQ=255)            ccATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGTCTTTCAGCGCCTCCAGTTCCACAGc                                                                                                                             >  2:273583/1‑143 (MQ=255)             cATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCt                                                                                                                            <  2:2969/143‑1 (MQ=255)                          actcttAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGa                                                                                                               <  2:550024/139‑1 (MQ=255)                            ttttAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGCgg                                                                                                             >  1:933047/1‑143 (MQ=255)                             tttAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAggg                                                                                                            >  1:925533/1‑143 (MQ=255)                                      gCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTg                                                                                                   >  1:852590/1‑143 (MQ=255)                                      gCAGGATGCGGCTGAAGTGAAGCCTGGGTCACAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGTCCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTg                                                                                                   <  1:526499/143‑1 (MQ=255)                                           aTGCGGTTGAAGCGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTGGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCtttt                                                                                              <  1:316082/143‑1 (MQ=255)                                            tGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCACTAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAACTGTATCCCCCTTGAGGGTGAGTTTTGCTTTTg                                                                                             >  1:345158/1‑143 (MQ=255)                                               ggTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAt                                                                                          >  1:795060/1‑143 (MQ=255)                                               ggTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTAt                                                                                          <  2:740003/143‑1 (MQ=255)                                               ggTTGAAGTGAAGCCTGGGTCAAACGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTAt                                                                                          <  2:739975/143‑1 (MQ=255)                                                      gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCAt                                                                                   >  1:243081/1‑143 (MQ=255)                                                          atccTGGGTCAAATGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTaa                                                                               <  2:316350/141‑1 (MQ=255)                                                           gCCTGGGTCAAAGGTGAACACCCCTCTTGAACCGAGAGGACGGATATCAATAACATCTTGACCCAGCGTGCCTGTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAg                                                                              <  1:46889/143‑1 (MQ=255)                                                                    aacggTGAACACACCTTTTGAAACGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                     <  2:73328/140‑1 (MQ=255)                                                                    aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGCATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTACGGTCTCCttc                                                                     >  2:379119/1‑142 (MQ=255)                                                                     aaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCTTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAg                                                                    <  1:762678/143‑1 (MQ=255)                                                                      attgtaaCACACCTTGTGAACCGAGGGTACGGATATCAATAACATCGTGACCTAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgc                                                                   <  2:848543/138‑1 (MQ=255)                                                                          gAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAACGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCt                                                               <  1:496951/143‑1 (MQ=255)                                                                           aaCACACCCTTTGCACCGAGAGTACGCATATCAATACCATCTTGCCCCAGCGCGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCtt                                                              <  1:958919/143‑1 (MQ=255)                                                                             cacacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCTCGTTGAGGGGGACGTTCGCTTTTGTATCAGCCATTTAAGCTTTCCTTCGCGCCttct                                                            >  1:983190/1‑141 (MQ=255)                                                                              acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGCGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCCTAtt                                                           >  1:373380/1‑143 (MQ=255)                                                                                acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCGTTAGGGCCCTATTgt                                                         >  2:173457/1‑142 (MQ=255)                                                                                acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                         >  2:96709/1‑143 (MQ=255)                                                                                acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCATTATTGc                                                         <  1:663363/143‑1 (MQ=255)                                                                                  cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCACCGTTGAGGGGGAGTTTTGCTTCTTTATCAGCCATTTAAGGTCTCCGTAGCCCCTTCTTGctt                                                       >  2:593167/1‑141 (MQ=255)                                                                                    tttGAACCGAGAGTACGCAGATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                     <  2:985402/143‑1 (MQ=255)                                                                                         aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTg                                                >  1:841478/1‑143 (MQ=255)                                                                                           cGAGAGTACGGATATGAATAACATCTTGACCCAGCGTGCCTTTCAGAGCATCCAGTTCAACAGCTGTATCCCGGTTGAGGGGGAGCTTTGCTTTTGTATAACCCATTTAAGGTCTCCTTAGCGTCTTATTGCGTGAGACTccc                                              >  1:204199/1‑143 (MQ=255)                                                                                           cGAGAGTACGCGTAGCCACACCATCTCGCCCCGGCGTGCGTTGCAGGGCATTCATTCCAACAGCTGCATCCACGGTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcc                                              <  1:454343/143‑1 (MQ=255)                                                                                                        atCAATTACATCGTCACTCGGCGTGCCTTTCAGCGCATCCAGTTCACCAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                 <  1:808631/143‑1 (MQ=255)                                                                                                        atCAATAACATCTTGACCCAGCGTGCCTTTCAGCGTATCCAGTTCAACAGCTTTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                 <  2:146024/143‑1 (MQ=255)                                                                                                        atCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAATACTTCCGGGACTTAAAttt                                 >  2:920552/1‑143 (MQ=255)                                                                                                                cATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTACGCAGCTGTTGGTCTGTTGATGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGc                         >  1:679914/1‑143 (MQ=255)                                                                                                                    ttGACACAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCGCAt                     >  1:432656/1‑143 (MQ=255)                                                                                                                       aCCCATCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                  <  2:846118/143‑1 (MQ=255)                                                                                                                       aCCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                  >  1:742470/1‑143 (MQ=255)                                                                                                                        cgcAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTGTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAAc                 <  1:831919/141‑1 (MQ=255)                                                                                                                           aGCGTGCCGTTCATCGCAGCCCGCTCACCAGCTGCATCCCCGTTGAGGGCGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTg              <  1:634480/143‑1 (MQ=255)                                                                                                                                gCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCttt         >  1:630855/1‑143 (MQ=255)                                                                                                                                   tttCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCGCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTCAATTTGCCCTCGCACATCAACCTGGCtttcgc      >  2:287568/1‑140 (MQ=255)                                                                                                                                       aGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTGGCCTTCGCCCATCAACCTGGCTTTACCCGtt  <  2:708998/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            TACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTT  >  NC_000913/754273‑754549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑