Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	762,329	Δ19 bp	coding (808‑826/1218 nt)	sucB →	dihydrolipoyltranssuccinase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	762329	762347	19	38 [0]	[0] 37	sucB	dihydrolipoyltranssuccinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 762328	0 (0.000)	37 (1.000)	29/282	0.3	100%	coding (807/1218 nt)	sucB	dihydrolipoyltranssuccinase
	?	NC_000913	762348 =	0 (0.000)					coding (827/1218 nt)	sucB	dihydrolipoyltranssuccinase

GGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAA  >  NC_000913/762206‑762480                                                                                                                                               |                                                                                                                                     attgtttgaaaaacgccaggggatccggctgggatttatgtccttctacgtgaaagcggtggttgaagcccggaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcc                                                                                                                                      <  1:610861‑M2/1‑1 (MQ=255)  gcgtttgaaaaacgccacggcatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtccg                                                                                                                                     >  1:299813‑M2/142‑143 (MQ=255)  gcgtttgaaaaacgccacggcatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtccg                                                                                                                                     >  1:63764‑M2/142‑143 (MQ=255)     tttgaaaaacgccacggaatatgtctgggctttatgtccttatacgtgaaaggtggggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtccgcgc                                                                                                                                  >  1:671997‑M2/139‑143 (MQ=255)          aaaacgccacggcatccgtctgggctttatgtccttctatgtgaaagcggtggttgaagccctgatacgtttcccggaagtgaacgcttctatcgacggcgatgacgtggtttaacacaactatttcgacgtcCGCGCGGCCt                                                                                                                             >  1:329289‑M2/134‑143 (MQ=255)           aaacgccacggcatccgtctgggctttatgtccttctccgtgacagcggtggttgaagtcatgaaacgttcaccggaagtgaacgcttcgatcgacggcgatgacgtggtttaccacaaccatctcgacgtccgcgccggccg                                                                                                                            >  2:146417‑M2/133‑137 (MQ=255)           aaacgccacggcatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTg                                                                                                                            >  1:580889‑M2/133‑143 (MQ=255)           aaacgccacggcatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTg                                                                                                                            >  1:518996‑M2/133‑143 (MQ=255)               gccacggcatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGa                                                                                                                        <  1:38827‑M2/15‑1 (MQ=255)                ccacgccatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGgtggc                                                                                                                       >  2:506786‑M2/128‑141 (MQ=255)                 cacggcatccgtctgggctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGGGCGGCCTGGTGACg                                                                                                                      >  1:88041‑M2/127‑143 (MQ=255)                    ggcatccgtctgggccttatgtccttatacgttaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCg                                                                                                                   <  1:633823‑M2/20‑1 (MQ=255)                    ggcacccgtctgggcgttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCg                                                                                                                   <  1:605215‑M2/20‑1 (MQ=255)                     gcatccgtctgggctttatgtcctgctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCgg                                                                                                                  <  1:129011‑M2/21‑1 (MQ=255)                                  ctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGt                                                                                                     >  2:20227‑M2/110‑143 (MQ=255)                                  ctttatgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGt                                                                                                     <  2:220709‑M2/34‑1 (MQ=255)                                      atgtccttctacgtgaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGAt                                                                                                 <  1:346551‑M2/38‑1 (MQ=255)                                                    gaaagcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGc                                                                                   <  1:383284‑M2/52‑1 (MQ=255)                                                       agcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGa                                                                                <  1:594801‑M2/55‑1 (MQ=255)                                                       agcggtggttgaagccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGa                                                                                <  1:110167‑M2/55‑1 (MQ=255)                                                            ttgttgaagccctgaagcgttacccggaagtgaccgcttttatcgatggctatcacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCg                                                                           <  2:619596‑M2/60‑1 (MQ=255)                                                            tggttgaagccctgaaacgttacccggaagtgcacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCg                                                                           <  1:654343‑M2/60‑1 (MQ=255)                                                            gggttgaagccctgacacgttacccggaagtgaacgcttctgtcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCg                                                                           <  2:287879‑M2/60‑1 (MQ=255)                                                              gttgaacccctgaaacgtcaccccgaagtgaacgcttctatcgacggcgacgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCgag                                                                         <  1:504140‑M2/62‑1 (MQ=255)                                                               ttgaagccctgaaacgttacccgcaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCgaga                                                                        <  1:499392‑M2/63‑1 (MQ=255)                                                                   agccctgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAgaagaa                                                                    <  2:149043‑M2/67‑1 (MQ=255)                                                                        tgaaacgttacccggaagtgaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCa                                                               >  1:426519‑M2/72‑143 (MQ=255)                                                                         gaagcgttacccggaagttaacgcttctatcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCTGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCaa                                                              <  2:192118‑M2/73‑1 (MQ=255)                                                                                       aagggaacggctctatcgacggcgatggcgtggtttaccacaactattgcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCa                                                <  2:518996‑M2/87‑1 (MQ=255)                                                                                         gtgaactcttctatcgatggctatgacgtattttaccacaactattacgacgtcCGCAAGGCCTGGAGACGCCGATCCTGCGTGATGTCGATACCCTCGCCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCaaa                                              <  1:329280‑M2/89‑1 (MQ=255)                                                                                            aacgcttctatcgacggcgatgacgtggtttaccacacctatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGCGAAGAAAACCAACGCGCCGGCAGTCACAGGc                                           >  2:91199‑M2/52‑143 (MQ=255)                                                                                                  tttttcgactgcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGAc                                     <  1:157851‑M2/98‑1 (MQ=255)                                                                                                   ctatcttcggctatgactttgtttatcacaactatttcgacatcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACg                                    <  1:111121‑M2/99‑1 (MQ=255)                                                                                                      tcgacggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa                                 >  1:372261‑M2/42‑143 (MQ=255)                                                                                                         acggcgatgacgtggtttaccacaactatttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGc                              >  1:555949‑M2/39‑143 (MQ=255)                                                                                                                                 actaattcgacgtcCGAGCAGCCGGAGAACGCAGGTTCTGCGTGATGTCGATGCCCTCGGGATGGCAGATATGGAGAAGAAAAGCAAAGAGCTGGAAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCggtg      <  1:126317‑M2/129‑1 (MQ=255)                                                                                                                                     tttcgacgtcCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGAccggtggtga  >  1:544515‑M2/11‑141 (MQ=255)                                                                                                                                               |                                                                                                                                     GGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAA  >  NC_000913/762206‑762480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑