| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 40.0% | 14.0 / 41.5 | 46 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (18/9); new base T (11/7); total (29/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC > NC_000913/4295926‑4296197 | ttATGCCGCAGCCGACATCCACGCCCGATGCGACGCTTAGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTAGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATg < 1:347263/143‑1 (MQ=11) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGACGCGACg > 1:347891/1‑143 (MQ=17) cccgccctCATCGTCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGc < 2:247676/136‑1 (MQ=11) aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCCACGCCTGATGCCACGCTTGCCGCGTc > 2:2746/1‑143 (MQ=255) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGTCTTa > 2:484056/1‑143 (MQ=25) aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGGCGGTTGGATGAGGCGTTTAAGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTcatgt > 2:549515/1‑139 (MQ=11) aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:31022/1‑143 (MQ=25) aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CACCACTGCCTGATGCGCCGCTTGCCGCGTCTTAt > 1:18385/1‑143 (MQ=255) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTCGCCGCGTCTtctc > 2:665246/1‑143 (MQ=255) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGCTGCGCCGCTTGCCGCGTCttcgcc > 1:162388/1‑139 (MQ=255) gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGAGGCGACGCTTGCCGCGTCTTATCAggac > 2:202069/1‑141 (MQ=255) aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGCCTTATAAggaca > 2:188494/1‑140 (MQ=255) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct > 2:178457/1‑140 (MQ=17) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt > 1:43174/1‑139 (MQ=9) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCGGCGTCTGATCGGGCTTGCGCCAgcc > 1:3289/1‑141 (MQ=11) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGAt > 2:8101/1‑106 (MQ=18) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGAt < 1:8101/106‑1 (MQ=18) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGAc > 2:548121/1‑143 (MQ=38) aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg > 1:530169/1‑143 (MQ=37) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGt < 1:495007/113‑1 (MQ=25) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGt > 2:495007/1‑113 (MQ=40) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa < 2:479564/127‑1 (MQ=40) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGa < 1:380236/92‑1 (MQ=21) cTACGCACGACAGCGCAATAGCATGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTGACGCCGCATCT‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa < 1:226560/143‑1 (MQ=14) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:204075/1‑95 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:204075/95‑1 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGCCAGCGCAATACCCTGCCTTCGCGTCACTTCCTAGGTCg > 2:7568/1‑143 (MQ=14) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGCGACGCTTGCCGCGTCTTa < 2:154310/86‑1 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTa > 1:154310/1‑86 (MQ=255) gCGTGATTTTGTGAGGCGGATAGGGCGTGTACGCCGCGTCC‑GG‑CGTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt < 2:463533/143‑1 (MQ=14) gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt > 1:582129/1‑143 (MQ=39) tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCATGTCTTTGCCAGACAGCGTAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg < 1:62244/143‑1 (MQ=11) gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTacgc > 1:358682/1‑140 (MQ=37) aTTTTTTAGGTGGGGTACGGCGTTTACCCCGCATCA‑GG‑CATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc < 2:166873/143‑1 (MQ=11) ttGTAGGTCGGATAAGGCGTTTCCG‑‑GCGTCCTGACCACTAGCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTCTCGCGTGCCTCTCTCGCTCGGATAAGGCGGTTATGCCGCa > 2:206685/1‑143 (MQ=2) tGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGa < 1:426270/47‑1 (MQ=255) tGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGa > 2:426270/1‑47 (MQ=40) ggATAAGGCGTTTACGCCGCATCC‑GA‑CATCAATGCCTGATGc < 1:344193/42‑1 (MQ=255) ggCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATGTTGTCGGGCGGATCAGGCGTTTATGCGGCCTCCGACGTCAGCGcc > 1:4461/1‑143 (MQ=11) gccgcATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 2:344629/1‑49 (MQ=255) ccgcATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt < 1:304855/108‑1 (MQ=38) cgcATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGc > 1:457673/1‑143 (MQ=35) cgcATCC‑GA‑CATCAACGCCTAATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGc > 1:564157/1‑143 (MQ=25) tCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCGTGACGGATCGTGATATTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACAGCAACGCCTGATGCGACGcgtga > 2:546059/1‑139 (MQ=11) cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt < 1:549515/143‑1 (MQ=35) gtCAAGGCCTGTGGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCTACATCAACGCCTGATGCGACGCTTAACGCGTc < 2:353236/142‑1 (MQ=11) | TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCC‑GA‑CATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC > NC_000913/4295926‑4296197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |