Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT32.2% 49.5 / 36.9 61intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (29/11);  new base T (12/7);  total (43/18)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTT  >  NC_000913/4295920‑4296199
                                                                                                                                            |                                                                                                                                           
aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                                                           <  2:703945/143‑1 (MQ=17)
  gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTGTGCAGGCCCGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                         >  2:911133/1‑143 (MQ=11)
    gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAACGCCTgc                                                                                                                                       >  1:240486/1‑142 (MQ=17)
    gTTTATGCCGCATCCGACATCAACGACTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGACACAGCCGACATCAATGCCTGa                                                                                                                                       >  2:125234/1‑143 (MQ=11)
     tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTCAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                      >  1:122286/1‑143 (MQ=17)
     tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                      <  1:21047/143‑1 (MQ=32)
     tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGTATAAGGCGATTACGCCGCAGCAGACATCAACGCCTGAt                                                                                                                                      >  2:512853/1‑143 (MQ=11)
           ccGCATCCGACATCAACGCCTGATGCGACGCTTAACTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                                                                >  1:703945/1‑143 (MQ=17)
            cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCGTACGCCAGACAGCGCAGTAGCCTGATTTAGCGTGATTTTGTAGGTCTGATGAGGCGTTCACGCCGCATCCGGCATCAATCCCTGCTGCGACGc                                                                                                                               >  1:723019/1‑143 (MQ=11)
             gCATCCGACATCAACGCCTGATGCGACGCTTAACGCATCTCATCAGCCCTACGCCAGACAGCGCAATACCCTGCTCTAGCGTCATTATGTAGATCGCATCAGCCGTTTCCGCCGCCTCCCACATCACGGCCGGACGCGgcgcg                                                                                                                              >  2:387208/1‑142 (MQ=2)
              cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                             >  2:298212/1‑143 (MQ=17)
               aTCCGACATCAACGCGTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACTTCAAACCCAGATGCGACGCCTg                                                                                                                            >  2:803899/1‑143 (MQ=11)
               aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCTACGCCTGTTGCGTCGCTTg                                                                                                                            >  1:245946/1‑143 (MQ=12)
                tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATGCCTGATGCGCCGCTTGc                                                                                                                           >  2:184041/1‑143 (MQ=16)
                tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                           <  1:667166/143‑1 (MQ=21)
                   gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCCTGCcgc                                                                                                                        >  1:281599/1‑143 (MQ=25)
                        cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                     <  2:776631/125‑1 (MQ=18)
                        cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                     >  1:776631/1‑125 (MQ=33)
                         aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTCTGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGACATCAACGCGCGACGCGACGCTGGCCGCGTCtcc                                                                                                                  >  2:672565/1‑141 (MQ=11)
                         aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAACGCCTGATGCGACGCGTGCCGCGTCCTa                                                                                                                  >  2:867035/1‑143 (MQ=255)
                         aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                  >  1:747389/1‑143 (MQ=37)
                         aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGACTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTACGCCGCATCCGCCATCCATCCCTGATGCGCCGCTGCCCGCGTCtcg                                                                                                                  >  1:293321/1‑141 (MQ=11)
                          aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTGTGCGCCGCATCCGACATCATTGCCTGATGCGACGCTTGGCCCGTcctct                                                                                                                 >  2:154165/1‑139 (MQ=11)
                          aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGTAGGTCGGATATGGCGTTTACGCCGCAACCGACATCAATGCCTGATGCGACCCTTGCCGCGTcctct                                                                                                                 >  2:881754/1‑139 (MQ=11)
                           cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATc                                                                                                                >  1:566803/1‑143 (MQ=25)
                               tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCGCTTATCAGGc                                                                                                            >  1:35201/1‑143 (MQ=255)
                                  tGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                                                         >  1:572163/1‑143 (MQ=21)
                                   gCGACGCTTAACGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCAGATACTGCTTTTTCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                        <  1:855437/143‑1 (MQ=14)
                                     gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTatct                                                                                                      >  1:231291/1‑140 (MQ=12)
                                      aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTTTCCGGCCtctctt                                                                                                     >  1:38441/1‑138 (MQ=11)
                                        gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGGCTTAGCAGGCCTACGCCAg                                                                                                   >  2:541233/1‑143 (MQ=255)
                                           tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                <  1:367490/140‑1 (MQ=37)
                                             aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTGCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGACAgc                                                                                              >  1:440851/1‑143 (MQ=255)
                                                       tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                           <  1:543375/104‑1 (MQ=25)
                                                       tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                       >  1:165501/1‑92 (MQ=34)
                                                       tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                                                                                                           >  2:543375/1‑104 (MQ=35)
                                                           cccgaCGCCAGACACCGCGATGGCCTGACTGCGCGTGATTTTGTGGGTCGGATAAGGCGTTTACGCCGCGCGCTGCGTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                                <  2:337429/139‑1 (MQ=14)
                                                              tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTACCCCAGACAGCGCTATCGCCTGCtttcc                                                                             >  2:891419/1‑141 (MQ=11)
                                                              tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                    >  1:642335/1‑120 (MQ=255)
                                                              gaCGCCGGACGGCGCAATGGCCTGACTGAGCGTGATTTTGTGGGGCGGACAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                                             <  2:892348/142‑1 (MQ=14)
                                                                   cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                             <  2:684764/106‑1 (MQ=38)
                                                                   cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                             >  1:684764/1‑106 (MQ=255)
                                                                      aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACCCCTGATGCGCCGCCGGCCGCGTCTTGTCAGGCCTACGCCAGACAGCGCAAAAGCCTGCTTTAGCGGGGttt                                                                     >  1:219561/1‑143 (MQ=11)
                                                                       tcgcgcAATAGCCCGATTTAGCGTGATGTTGTAGGTCGGGTAAGGCGTTTACGCCGCATCCGACATCAGCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                                                                    <  2:240486/141‑1 (MQ=255)
                                                                             aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGGAGTAGCCTGCTGTGGCGTGCTGTTGTAgga                                                              >  2:33642/1‑142 (MQ=11)
                                                                               tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                             >  2:154459/1‑94 (MQ=17)
                                                                               tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                             <  1:154459/94‑1 (MQ=255)
                                                                                  ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                     <  1:516112/67‑1 (MQ=255)
                                                                                         tAGCGTGATTTTGTAGGGCGGATAAGGCGTCTCCGCCGCACCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                  <  1:646706/143‑1 (MQ=255)
                                                                                              tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGCTTTAGCGTCATTCTGGATATCGGACAAGGCgctcacg                                             >  2:449383/1‑137 (MQ=14)
                                                                                                 ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGTCAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGTATACTGCGTTTATGGCg                                          >  1:44053/1‑143 (MQ=14)
                                                                                                        ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg                                                               <  1:878553/115‑1 (MQ=255)
                                                                                                         gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                               <  2:689020/50‑1 (MQ=255)
                                                                                                                     gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTCATTTCGTGTATCGGATCCGGCGATAATGCCCCGTCCGACATCAACCCATGa                      >  2:653890/1‑143 (MQ=14)
                                                                                                                     gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATGCGACATCCACGCGTgg                      >  2:841729/1‑142 (MQ=255)
                                                                                                                        cgcgccgcTTCCGACATCATCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                   <  2:747389/141‑1 (MQ=255)
                                                                                                                           gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTCTCACGCCTACCTCAGACGGCGCAATAGCCTTCTTTACCGTGATTCCGTGTGTCGGATAAGCCGTGTCTGCCGCATCTCACCCCAACGCCTGAcgcgtc                >  2:155284/1‑141 (MQ=2)
                                                                                                                                       aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt                                                                     <  2:569569/78‑1 (MQ=39)
                                                                                                                                       aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGGCGCTTCCCGCGTc    >  2:942066/1‑143 (MQ=255)
                                                                                                                                        tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt   >  1:661460/1‑143 (MQ=33)
                                                                                                                                         cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGAGACGCTTACCGCGTCtt  >  2:695518/1‑143 (MQ=17)
                                                                                                                                            |                                                                                                                                           
AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTT  >  NC_000913/4295920‑4296199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: