New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3263490	56 (1.460)	3 (0.080)	3/280	5.7	5.1%	coding (171/1209 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic
	?	NC_000913	= 3263511	56 (1.480)					coding (150/1209 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAA  >  NC_000913/3263373‑3263651                                                                                                                                           |                                                                                                                                             cTCGGTAAAAATAAGGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTTCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                          <  1:225365/143‑1 (MQ=255)     gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCCCTcccccgtc                                                                                                                                      >  1:335853/1‑140 (MQ=255)     gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAtt                                                                                                                                      >  1:460049/1‑143 (MQ=255)          aaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACgg                                                                                                                                 >  1:463483/1‑143 (MQ=255)           atcctgctgCCTTGATCGATGCCGTGGTCAATTAACGACACATTGTCATTTCAACTCCGTTTTTCAAGTTACAATGCACATGCCTCCACTACACCCACGCAACTGTAGACCGTCAGCGGTGCTGGCTCTCCCCCATTTACGGa                                                                                                                                <  2:649139/140‑1 (MQ=255)               tGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGGGCTGGCTCTCCCCCATTTACGGATAAg                                                                                                                            >  1:185656/1‑143 (MQ=255)                 ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGaa                                                                                                                          >  1:240754/1‑143 (MQ=255)                   gccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg                                                                                                                        <  1:168763/143‑1 (MQ=255)                       tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATCTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAtt                                                                                                                    <  1:29052/143‑1 (MQ=255)                         aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCACATGCAGATGACTTAAGTGCACCTTCGTAGCTGTGGTGCGCAAGCGGTGATGGGTCTCCCCCATTTACGGATAAGAATGCAtttt                                                                                                                  <  2:279874/143‑1 (MQ=255)                             aTGCGTTTGCCAATTACGGCCACACTGTCATTTAAATGCCGTGTTTCCAGTTCAAATGCGATTGCCTTCAGTGCACCTTCGTAACTGTGCTGAGCCAGCGGTGCTCGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                              <  2:421467/143‑1 (MQ=255)                                  gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTaa                                                                                                         >  1:686273/1‑143 (MQ=255)                                    ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                                       <  1:633740/143‑1 (MQ=255)                                     gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCTGAGTTAATAc                                                                                                      >  1:193431/1‑143 (MQ=255)                                     gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAc                                                                                                      >  1:466260/1‑143 (MQ=255)                                     gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATCTACGGCTCAGCATGCATTTTCCGAGTCAATAc                                                                                                      >  2:260065/1‑143 (MQ=255)                                      ccAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTCAATAcc                                                                                                     >  1:410712/1‑143 (MQ=255)                                       cAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCCCCCCCATTTACGGCTAAGAATGCGTTTTCCGAGTTaaggccg                                                                                                    >  1:186723/1‑138 (MQ=255)                                          ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                                                 <  2:584547/143‑1 (MQ=255)                                          ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATACAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGttc                                                                                                 >  2:183466/1‑141 (MQ=255)                                           tAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCACTTGCCCTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                <  2:335118/143‑1 (MQ=255)                                           caaGGCCACACTGTCATTTAAATTCCGTTGTTCCAGTGCAAATGCAATTGCCTTCAATGCACCTTCGTCGCTGTGGTCATCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                <  2:458747/142‑1 (MQ=255)                                            aaGGCCCCACTGTCGTCTAAATTCCGTTTTTTCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTTGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                               <  2:106104/143‑1 (MQ=255)                                             aGGCCACACTGTCATTTAAATTCCCTTTTTCCAATTGAAATGCAATTGCCTTCAATGCACCTTCGTACATATAGTATACGATTTGTTTTGGCTCGCCCCCAGTTACGGGGGAGAATGCAGTTTCCGAGTTAAGACCGTCGGCa                                                                                              >  2:208170/1‑143 (MQ=255)                                               gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAACGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCGTTTTCCGCGTTCATACCGTCGGCCAt                                                                                            >  1:337578/1‑143 (MQ=255)                                               gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCAGTGGTGAGCCAGCGATACCGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGCTAATACCGTCGGCaaa                                                                                            >  2:242794/1‑142 (MQ=255)                                                 cacaCTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTACTACCGTCGACAATAc                                                                                          >  2:668669/1‑143 (MQ=255)                                                    acTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTTTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACGGCTCCTAATGCATTTTCCCAGTTAATACCGTCGGCCCTACCTg                                                                                       >  1:633016/1‑143 (MQ=255)                                                             tAAAGTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                              <  1:617573/143‑1 (MQ=255)                                                                  tCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGTCGGCAATACCTGCCAGTAATACTTca                                                                         >  2:672870/1‑143 (MQ=255)                                                                    cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGCATGCATTGTCCGAGTTCATACCGACCGCGCTACCTGACGTTATTACTTcatc                                                                       >  2:128966/1‑141 (MQ=255)                                                                     gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATCCCTGACATTAATACTTGACAg                                                                      >  1:575599/1‑143 (MQ=255)                                                                          tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                                                 <  2:131786/143‑1 (MQ=255)                                                                           ccAGTTCAAATGCAATTGTCTTCAATGCACCTTCGCAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                                <  1:621590/143‑1 (MQ=255)                                                                            cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                               >  1:80893/1‑143 (MQ=255)                                                                            cAGTCCAAATGCACTTGCCTTCGTTGTACCTTCGTAGCTGTGGTGAGGCAGCGGTGCTGGCTCTCCTCCATTTACGTATACGAATTCGTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                               <  2:208531/143‑1 (MQ=255)                                                                               ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCAt                                                            >  1:678000/1‑143 (MQ=255)                                                                               ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATGAATACTGCACAGTCGCTGGCAt                                                            >  1:644673/1‑143 (MQ=255)                                                                                  aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACGTTGATACTTCACAGTCGCTGGTGTCGa                                                         >  1:13074/1‑143 (MQ=255)                                                                                           tGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaa                                                >  2:18026/1‑143 (MQ=255)                                                                                             ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATACAATTACCAATTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAc                                              <  1:97687/143‑1 (MQ=255)                                                                                                tgaacccACCTTCGTAGATGTGGTGAGCCCGCGGTTCTGGCCCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTa                                           <  1:401122/137‑1 (MQ=255)                                                                                                 ccttGCACCTGCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                          <  2:380384/140‑1 (MQ=255)                                                                                                 cAACGCACCTGCGTAGGTGTGCTGAGCCAGCGGTGCTGGTTCGCCCCCACTTACGAATAAGAAAGCATATTCCGAGTTAAGACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                          <  2:81124/143‑1 (MQ=255)                                                                                                   aTGCACCTTCGTGGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATc                                        <  1:128460/143‑1 (MQ=255)                                                                                                     gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGATAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGACAACTTAATCGa                                      >  1:408686/1‑143 (MQ=255)                                                                                                          ttCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCGATACCTGACATTACTACTTCACAGTCGCTGGCATCGAGCACGGAAAACTCAATCGAAGCCg                                 >  1:31076/1‑143 (MQ=255)                                                                                                                 cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGACAACTTAATCGAGGACGAACcaca                          >  1:200845/1‑143 (MQ=255)                                                                                                                    tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                       >  1:78630/1‑143 (MQ=255)                                                                                                                     ggagAGCCAGCACCGCTGGCACTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGTCGGCCATACCTGACATTAATACTTCACAGTCGCTGCCATCGAGCACGGAAAAGTTCATCGAAGACGCACCACAATta                      >  2:367109/4‑143 (MQ=255)                                                                                                                        gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata                   <  2:694016/143‑1 (MQ=255)                                                                                                                           ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAAGGCATTTTCCGAGTGAATACCGTCGGCAGTACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAGAACTTAATCGAAGACGAACCACAGTTAATAAcc                >  1:214944/1‑143 (MQ=255)                                                                                                                              gCGGTGCCGGCTCTCCCCCATTTATGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaa             <  1:387774/143‑1 (MQ=255)                                                                                                                               cGGTGCTGGCTCTCCCCCATGTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTGCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaaa            <  2:319990/143‑1 (MQ=255)                                                                                                                                         tctcCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaaa  >  1:577564/1‑143 (MQ=255)                                                                                                                                         tctcACCCATTTACGGATAAGAATCCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaaa  <  1:696552/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                             CTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAA  >  NC_000913/3263373‑3263651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.