| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 702,066 | G→C | 54.8% | P229R (CCT→CGT) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 702,066 | 0 | G | C | 54.8% | 0.1 / 53.8 | 31 | P229R (CCT→CGT) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base C (8/9); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01 | |||||||||||
CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGT > NC_000913/701781‑702328 | cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGt > 2:251794/1‑288 (MQ=255) tCGACCAGATTACGCACCCCTTCAATCATGGTTAAGGCTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGAc < 2:149598/289‑1 (MQ=255) gACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCg > 2:114027/1‑289 (MQ=255) caagcattccacacgcCTTCAATCAGGGTTAAGGATGACCCGCTTACCGTACCTTTACCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCgg < 2:52435/278‑1 (MQ=255) aCCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCgg < 2:19124/289‑1 (MQ=255) ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATc > 2:179922/1‑288 (MQ=255) ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGACCGCCAGGCCAGGTTCACGACCGGTAATATAAGGccttt > 1:52435/1‑285 (MQ=255) tacAATCATGGTTAATGATGAACCACTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg < 2:88699/287‑1 (MQ=255) ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGa > 2:107813/1‑289 (MQ=255) gAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCaa < 1:251794/289‑1 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGTCGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCAAGGCCACTGTCACGACCGCTTATATCCGGCATCACGTGGTAGAGAGGGGTGGCgaa > 2:66088/1‑289 (MQ=255) aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATcgcg < 1:239995/268‑1 (MQ=255) aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATcgcg > 2:239995/1‑268 (MQ=255) cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGtt > 1:182259/1‑266 (MQ=255) cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGtt < 2:182259/266‑1 (MQ=255) ttttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATCCGGCATCGCGTTGTACAGATGGGTGGCAACGGTAATCCCCGAGCCGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTg > 2:53494/1‑289 (MQ=255) ttCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa > 2:96423/1‑188 (MQ=255) ttCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa < 1:96423/188‑1 (MQ=255) tGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCa > 2:167782/1‑269 (MQ=255) tGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCa < 1:167782/269‑1 (MQ=255) gCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg < 2:190388/183‑1 (MQ=255) gCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg > 1:190388/1‑183 (MQ=255) aaccagtaccaGTTTTTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGa < 1:254421/277‑1 (MQ=255) cGTTGAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGt < 1:114027/287‑1 (MQ=255) tAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAg > 2:12953/1‑252 (MQ=255) tAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAg < 1:12953/252‑1 (MQ=255) gAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGt < 1:179922/288‑1 (MQ=255) ccATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAgcg < 1:107813/286‑1 (MQ=255) aaaTGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTCGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAAtt < 1:53494/289‑1 (MQ=255) aGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAACTCGACCAGCGAGGCATCAGGCTTACGcacacactttggtata > 2:278740/1‑277 (MQ=255) cgtcgAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGt > 1:103930/1‑289 (MQ=255) | CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGT > NC_000913/701781‑702328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |