| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 91.7% | 137.8 / 4.7 | 36 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base . (17/16); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 94.3% | 135.2 / 2.4 | 36 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (17/16); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTA > NC_000913/2173219‑2173487 || aGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccac > 1:335180/1‑143 (MQ=255) cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGGCATGCCGATaccaccgccgta > 1:325229/1‑137 (MQ=255) aCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCcag > 1:315973/1‑143 (MQ=255) aCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcag < 1:270215/143‑1 (MQ=255) cGGCGCCCGCCACCGCCACGAAGGCGTCAATGGGGGCGGGGTCGTCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagc < 1:160116/143‑1 (MQ=255) cGGCGACCGCCATCGCCGCGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATCAACATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagc < 1:168760/143‑1 (MQ=255) ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGAACa > 2:638586/1‑143 (MQ=255) cGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGGGA‑‑CGCCGGACCACgg > 2:410638/1‑143 (MQ=255) aagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGcc > 1:680308/1‑143 (MQ=255) gCCGATGGTGGAAGGAGAGCGAAGCGGCAGCACCTGATTACCCGGCACACAAACAGCAATGAAAATGGCGAGTGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCa < 1:391041/143‑1 (MQ=255) ccGATGGGGGCAAGCTCGCCGTACGGCAGCACCAGCCTCAACAGCGCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAg < 1:401053/143‑1 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCAGTATGTCCCAGAAGCAA‑‑CGCCGGATCAAGGCCAATCAgg > 1:751750/1‑143 (MQ=255) cGAGGGTGGCAAGATCGTCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAgg < 2:237908/143‑1 (MQ=255) gtggtgtGAAGATCGCCAAACCGCAGCACCGGATTCCCCGGCACACAAACAGCAATTAAAATGGCGAGTGGGATAAAATTCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGaa < 2:348615/142‑1 (MQ=255) ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCACTCAGGAACt > 2:321604/1‑143 (MQ=255) gtgaCACGATGGCCAACCGGAAGCACCTGATTACCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCAGATCAAGGCCAATCAGGAACTc < 2:869597/143‑1 (MQ=255) tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTcc > 1:141294/1‑143 (MQ=255) ggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTTGTGTGCCGAGAGCGAGAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCt < 1:875020/143‑1 (MQ=255) gATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAACAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGAccg > 2:388067/1‑143 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCAGGATCAAGGCGAGTGAGCAACTCCTGAcggc > 2:757520/1‑143 (MQ=255) gtcagcaCCCGACTCCCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGATCAAAATCACGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 1:284760/141‑1 (MQ=255) cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCCGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCCATCAGGAACTCCTGACCGCCGCACTTCGccc > 2:845106/1‑142 (MQ=255) cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGACTAGCCGATACAA‑‑CCACTGTATGT‑AAAGC‑GCATTTCCGCGGATCCAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGccc > 2:857589/1‑142 (MQ=255) cgcgATTACCCGGAACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaaa < 1:464224/140‑1 (MQ=255) gATTCCCCTGCACACGAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAGTTCGCCTGTAAACGa < 2:742777/143‑1 (MQ=255) tACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTCTGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTa > 1:306612/1‑143 (MQ=255) ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTCAACGACTACGa > 1:621466/1‑143 (MQ=255) ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGCATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa > 2:845678/1‑143 (MQ=255) ggccgccaAACAGCACGTAAAAGGGTGAGTGGGATAAACGTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc < 2:670900/137‑1 (MQ=255) aCAGCAATTAAAATGGTGAGTGGGAGAAAAATCAGGCTTGCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGc < 1:314782/143‑1 (MQ=255) aGCAATTAAAATGGTGAGTGGGCTCACAATCAGGCTTGCCTATACCA‑‑CCGCCATATGTCCAAACAACAA‑‑AGACGAATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTCGCGa > 1:611735/1‑143 (MQ=255) cAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTACTCGACAACGAGCCTCCTTAGCGGTg > 2:415551/1‑143 (MQ=255) aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc < 1:590217/143‑1 (MQ=255) tAAAAACCAGGCTCGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGACGGACCAAGGCCAAGCCGTAACCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg < 2:478067/143‑1 (MQ=255) aaaaaTCAGGCTCGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg < 1:757520/143‑1 (MQ=255) aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCACGGCCAATCCGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGACGGACGTTAAACCATCCACGATggat > 2:693448/1‑141 (MQ=255) aaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt < 1:213514/143‑1 (MQ=255) tCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa < 1:415453/143‑1 (MQ=255) || AGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAA‑‑CGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTA > NC_000913/2173219‑2173487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |