Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,509,479 | C→T | intergenic (‑53/‑151) | glk ← / → yfeO | glucokinase/putative ion channel protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,509,479 | 0 | C | T | 97.7% | 124.6 / NA | 43 | intergenic (‑53/‑151) | glk/yfeO | glucokinase/putative ion channel protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (23/19); minor base A (1/0); total (24/19) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAT > NC_000913/2509340‑2509617 | cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAGCTGCTCCGCTTAAGTCACAATAATTCTTTCTCACACTGTAATTACTTgg > 1:143460/1‑143 (MQ=255) gCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAGTACTTgggg > 1:182614/1‑143 (MQ=255) tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaa < 1:117778/143‑1 (MQ=255) tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaa < 1:190062/143‑1 (MQ=255) accacTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTCAAACTGCTCCGCTAAAGTCAAACTAATTCTTTCTCACACTGTCCATACTTGGGGGAATaacaac > 2:868892/1‑143 (MQ=255) cgcTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaacaacaa < 2:635339/141‑1 (MQ=255) tGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGc > 1:396294/1‑143 (MQ=255) gCAATATCACACAGAGCGAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCg < 2:874733/143‑1 (MQ=255) atatCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGACATCACAACAACGCCCGaa > 1:651747/1‑143 (MQ=255) cacaGGGCCAGCGGTGCGGTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATTATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcag < 1:482711/143‑1 (MQ=255) cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcag < 1:387459/143‑1 (MQ=255) acaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcagg > 2:287342/1‑143 (MQ=255) gACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTa < 2:428536/143‑1 (MQ=255) tGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGAATAAAAACAACGCGGGAAAGGCAGGCTACGTGTAAata > 1:2190/1‑143 (MQ=255) gCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAatat < 1:275132/143‑1 (MQ=255) gccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg < 1:345849/143‑1 (MQ=255) cccACATCACCGACTAATGCATACTATGAAATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCCAGCTCCCTTTAATGATCGAACTGGGtgc > 2:64686/1‑141 (MQ=255) tCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAACACTGGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt > 2:659951/1‑143 (MQ=255) cgtcTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt < 1:579119/140‑1 (MQ=255) gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt > 1:253377/1‑143 (MQ=255) tAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAAAATCGATCTGGGTAACACAATAACTTTAt > 1:437724/1‑143 (MQ=255) gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACATGAGGCCATAACAACACCGCCGGAAATGCAGGCTCCCTGTAAATATCGATCCGGGTCACACAATCGCTTTATCgct > 2:476428/1‑141 (MQ=255) gTCATTCTTCAACAGCACCGCTAAAGGCAAAATAATTCCGTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATACCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCa < 1:42201/143‑1 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACCACGCCGGAAAGGCAGGCTCCCTGTACATATCGATCTCGGTCCCCCACTTACTTTATCGTTTCAGCACCACtt > 1:582725/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtc > 1:464964/1‑142 (MQ=255) ttcttcAACTACTCAGCTAAAGTCAAAATAGTTCTTTCTCACACTGTAAATACTTGGAGACGTAAAAACAACGCCGGAGAGGCAGGCTCCCTGTAAAAATCGATCGGGGGCACACAATTACTTTATCGTTTCAGCACCAGTTg > 2:504476/1‑143 (MQ=255) ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTCACTACTTGGGGGCATAACAACAACGCCGGAAAGGCAAGCTCCCTGTACATATCGATCTGCGTCACAACACTCCGTTGTCGTTGTAGCACCAATGGCAg > 2:805734/1‑143 (MQ=255) aCTGCTCCGCTAAAGTCAAAATAATTCTTTCTCTCACTGTAAATTTTTGGGGTGCGCACAACAACGGCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGAt < 1:199942/143‑1 (MQ=255) tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc < 1:256703/143‑1 (MQ=255) tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAACACTTCGTGGCATAACACCACCACCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc < 2:8944/143‑1 (MQ=255) cTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTCTCGTCTCAGCACCACTTGCAGCGAGGCCt > 2:278320/1‑143 (MQ=255) cTCCGCTAAAGTCAAAATAAGTCTTGCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGATAGGCAGGCTCCCTGTAAATATCGATCTAGGTCACACAATACCTTTATCGTTTCAGCACCAATTGCAGCAATGCCt > 2:439116/1‑143 (MQ=255) tCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGACAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTCTATCGTTTCCGTACAAATTGCACAGATGCCtt > 2:217671/1‑143 (MQ=255) gTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGc > 1:604246/1‑143 (MQ=255) aaaaTAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg > 1:751618/1‑143 (MQ=255) ctttctCACTCTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAACTATCGATCTTGGTCCCCCACTTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 1:877900/143‑1 (MQ=255) ctttctCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 1:148766/143‑1 (MQ=255) ttctCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGTTCACACAATTACTTTCTCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGt < 1:287342/143‑1 (MQ=255) cacatcgTAAAGACTTGGGGGCATAACAACGACGCCGTAAATGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 1:684360/137‑1 (MQ=255) cTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcc < 1:863320/143‑1 (MQ=255) tGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCGCTGTAAATATCGATCTGGGTCACACCATTACTTTATCGTTTCAGCACCACTTGAAGCGATGCCTTTTTGCACCCTGGGCAATCTAAGTATCTGAccc > 2:111419/1‑143 (MQ=255) tAAATACTTGGGGGCATAACAACAACGCCGGAAAGGAAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCg > 1:570167/1‑143 (MQ=255) aTACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATCACTATATCGGTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAACGCAAGTATCTCACCCCTCag > 1:715859/1‑142 (MQ=255) | CTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAT > NC_000913/2509340‑2509617 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |