Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,509,479 C→T intergenic (‑53/‑151) glk ← / → yfeO glucokinase/putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,509,4790CT97.7% 124.6 / NA 43intergenic (‑53/‑151)glk/yfeOglucokinase/putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/19);  minor base A (1/0);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAT  >  NC_000913/2509340‑2509617
                                                                                                                                           |                                                                                                                                          
cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAGCTGCTCCGCTTAAGTCACAATAATTCTTTCTCACACTGTAATTACTTgg                                                                                                                                         >  1:143460/1‑143 (MQ=255)
  gCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAGTACTTgggg                                                                                                                                       >  1:182614/1‑143 (MQ=255)
        tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaa                                                                                                                                 <  1:117778/143‑1 (MQ=255)
        tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaa                                                                                                                                 <  1:190062/143‑1 (MQ=255)
            accacTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTCAAACTGCTCCGCTAAAGTCAAACTAATTCTTTCTCACACTGTCCATACTTGGGGGAATaacaac                                                                                                                             >  2:868892/1‑143 (MQ=255)
              cgcTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaacaacaa                                                                                                                           <  2:635339/141‑1 (MQ=255)
                 tGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGc                                                                                                                        >  1:396294/1‑143 (MQ=255)
                   gCAATATCACACAGAGCGAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCg                                                                                                                      <  2:874733/143‑1 (MQ=255)
                      atatCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGACATCACAACAACGCCCGaa                                                                                                                   >  1:651747/1‑143 (MQ=255)
                            cacaGGGCCAGCGGTGCGGTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATTATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcag                                                                                                             <  1:482711/143‑1 (MQ=255)
                            cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcag                                                                                                             <  1:387459/143‑1 (MQ=255)
                             acaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcagg                                                                                                            >  2:287342/1‑143 (MQ=255)
                                      gACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTa                                                                                                   <  2:428536/143‑1 (MQ=255)
                                          tGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGAATAAAAACAACGCGGGAAAGGCAGGCTACGTGTAAata                                                                                               >  1:2190/1‑143 (MQ=255)
                                           gCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAatat                                                                                              <  1:275132/143‑1 (MQ=255)
                                                   gccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg                                                                                      <  1:345849/143‑1 (MQ=255)
                                                       cccACATCACCGACTAATGCATACTATGAAATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCCAGCTCCCTTTAATGATCGAACTGGGtgc                                                                                  >  2:64686/1‑141 (MQ=255)
                                                             tCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAACACTGGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt                                                                            >  2:659951/1‑143 (MQ=255)
                                                                 cgtcTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt                                                                        <  1:579119/140‑1 (MQ=255)
                                                                  gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt                                                                       >  1:253377/1‑143 (MQ=255)
                                                                     tAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAAAATCGATCTGGGTAACACAATAACTTTAt                                                                    >  1:437724/1‑143 (MQ=255)
                                                                         gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACATGAGGCCATAACAACACCGCCGGAAATGCAGGCTCCCTGTAAATATCGATCCGGGTCACACAATCGCTTTATCgct                                                                >  2:476428/1‑141 (MQ=255)
                                                                                  gTCATTCTTCAACAGCACCGCTAAAGGCAAAATAATTCCGTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATACCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCa                                                       <  1:42201/143‑1 (MQ=255)
                                                                                     aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACCACGCCGGAAAGGCAGGCTCCCTGTACATATCGATCTCGGTCCCCCACTTACTTTATCGTTTCAGCACCACtt                                                    >  1:582725/1‑143 (MQ=255)
                                                                                     aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtc                                                    >  1:464964/1‑142 (MQ=255)
                                                                                      ttcttcAACTACTCAGCTAAAGTCAAAATAGTTCTTTCTCACACTGTAAATACTTGGAGACGTAAAAACAACGCCGGAGAGGCAGGCTCCCTGTAAAAATCGATCGGGGGCACACAATTACTTTATCGTTTCAGCACCAGTTg                                                   >  2:504476/1‑143 (MQ=255)
                                                                                         ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTCACTACTTGGGGGCATAACAACAACGCCGGAAAGGCAAGCTCCCTGTACATATCGATCTGCGTCACAACACTCCGTTGTCGTTGTAGCACCAATGGCAg                                                >  2:805734/1‑143 (MQ=255)
                                                                                             aCTGCTCCGCTAAAGTCAAAATAATTCTTTCTCTCACTGTAAATTTTTGGGGTGCGCACAACAACGGCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGAt                                            <  1:199942/143‑1 (MQ=255)
                                                                                               tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc                                          <  1:256703/143‑1 (MQ=255)
                                                                                               tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAACACTTCGTGGCATAACACCACCACCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc                                          <  2:8944/143‑1 (MQ=255)
                                                                                                 cTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTCTCGTCTCAGCACCACTTGCAGCGAGGCCt                                        >  2:278320/1‑143 (MQ=255)
                                                                                                 cTCCGCTAAAGTCAAAATAAGTCTTGCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGATAGGCAGGCTCCCTGTAAATATCGATCTAGGTCACACAATACCTTTATCGTTTCAGCACCAATTGCAGCAATGCCt                                        >  2:439116/1‑143 (MQ=255)
                                                                                                  tCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGACAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTCTATCGTTTCCGTACAAATTGCACAGATGCCtt                                       >  2:217671/1‑143 (MQ=255)
                                                                                                           gTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGc                              >  1:604246/1‑143 (MQ=255)
                                                                                                              aaaaTAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg                           >  1:751618/1‑143 (MQ=255)
                                                                                                                       ctttctCACTCTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAACTATCGATCTTGGTCCCCCACTTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa                  <  1:877900/143‑1 (MQ=255)
                                                                                                                       ctttctCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa                  <  1:148766/143‑1 (MQ=255)
                                                                                                                         ttctCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGTTCACACAATTACTTTCTCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGt                <  1:287342/143‑1 (MQ=255)
                                                                                                                             cacatcgTAAAGACTTGGGGGCATAACAACGACGCCGTAAATGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt            <  1:684360/137‑1 (MQ=255)
                                                                                                                                 cTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcc        <  1:863320/143‑1 (MQ=255)
                                                                                                                                  tGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCGCTGTAAATATCGATCTGGGTCACACCATTACTTTATCGTTTCAGCACCACTTGAAGCGATGCCTTTTTGCACCCTGGGCAATCTAAGTATCTGAccc       >  2:111419/1‑143 (MQ=255)
                                                                                                                                    tAAATACTTGGGGGCATAACAACAACGCCGGAAAGGAAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCg     >  1:570167/1‑143 (MQ=255)
                                                                                                                                       aTACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATCACTATATCGGTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAACGCAAGTATCTCACCCCTCag  >  1:715859/1‑142 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAT  >  NC_000913/2509340‑2509617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: