| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 417,391 | G→C | A84P (GCC→CCC) | phoB → | response regulator in two‑component regulatory system with PhoR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 417,391 | 0 | G | C | 98.3% | 164.9 / ‑6.1 | 58 | A84P (GCC→CCC) | phoB | response regulator in two‑component regulatory system with PhoR |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (31/26); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
CTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGA > NC_000913/417254‑417527 | ttgtgAATCAACAGAAGGAACCCTGGCCGGATTTAATGCACCACGACTGGATGTGACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCaga < 1:378039/142‑1 (MQ=255) gAATCGACTGCTTGGACCCTGGCCCGTTTTGATTCTCCTCGTCTGTATGTTACCTGGCGGCGCCGGTATCCAGTGCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAgggg < 2:472558/143‑1 (MQ=255) aaTCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGga > 1:862829/1‑143 (MQ=255) tctgcTGAATGCACCCTGGTCGGGTTCAATTCCGCTCTGCTGTGTCTGCTCCGCCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGGGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaag < 2:640711/139‑1 (MQ=255) tCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGCGGGgaag > 2:589275/1‑143 (MQ=255) aaCTGAGGGAACCCTGGCCGGCTTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaa < 2:95770/143‑1 (MQ=255) aaCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaa < 1:448112/143‑1 (MQ=255) aaCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGAGGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaa > 1:76715/1‑143 (MQ=255) aCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACTCGGCGGCTCCGGTATCCAGTTCATCAAACACCTCACGCGCGAGACGATGACGCGGGATATTCCAGTGGTGATGTTGACGCCCGGAGGGcaaggag > 2:348226/1‑143 (MQ=255) aCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaag < 2:457309/143‑1 (MQ=255) cTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATGCAGTTCATCAAAAACCTCAAGCGCGAGTCGATGACCCGGGATATTGCAGTGGTCGTGTTGACCGCCGCAGGGgaaggaga > 1:147845/1‑143 (MQ=255) tgaatgaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaagaa < 2:697992/143‑1 (MQ=255) tgaatgaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaagaa < 1:815520/143‑1 (MQ=255) gaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGaagaagaagcacg > 2:765017/1‑139 (MQ=255) cTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTGATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTgc > 1:668900/1‑143 (MQ=255) cTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTgc > 1:340709/1‑143 (MQ=255) aaTTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGCCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGCCAGAAGAAGTGCGCGTGCGCGGCCTTGaaa > 1:325079/1‑143 (MQ=255) gtatcttcGACTGGATGTTACCTGGCTGCTCCGGTCTACTAGCCATCAAACACCTCAAGCTCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAAcc < 2:181897/137‑1 (MQ=255) tcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTTCAGTGGTGATGTTGACCCCCAGACGGGAGGCAGAAGATCGCGAGCGCGTCCTTAAACGCGGagcg > 2:30637/1‑143 (MQ=255) tcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGcgcg > 1:34770/1‑143 (MQ=255) cGACTGAATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATCCCAGTGGTGAGGTTGACCCCAAGAGGGGAAGAAGAAGATCGCGTGCGGGGCCTTGAACCAGGCGAgg > 2:819286/1‑143 (MQ=255) aCTCGAGGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAGGCGCGAGTCGATGGCCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGAGCGCGTGCGCGGCCTTGAAACCGGCGCGGAt < 2:354796/143‑1 (MQ=255) gggAGGTTCCCGGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGa < 2:610259/142‑1 (MQ=255) ggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGAACCCCAGAGGGGAAGCAGAAGATCGCGTGAGCGGCCTTGAGACCGGCGAGGCTGACTGTATccccgaccc > 1:173411/1‑138 (MQ=255) tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGCAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCACGGATGACTATATCACCAAGCCGtt > 2:367166/1‑143 (MQ=255) tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATAACCACGctgcg > 1:590711/1‑139 (MQ=255) cGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGtttt > 2:647095/1‑143 (MQ=255) ccAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGa > 1:187776/1‑143 (MQ=255) ccAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGa < 2:187776/143‑1 (MQ=255) aGTTCAGCAGCCGCCTCAAGCGCGAGTCGATGACCCGGGATATCCCAGTGGTCAGGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAg < 2:688865/143‑1 (MQ=255) cacacacCTCCCGCGCGAGTCGCTGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGCGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCCAGCCGTTTTCGCCGAAGGAGCtgg < 1:127282/140‑1 (MQ=255) aaaCACCTCAAGCGCGAGTCGATGACACGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATGTCACCCAGCCGTGTGCGCAGCAGGAGCcggc > 2:837788/1‑142 (MQ=255) ccgcAAGCGCGAGTCGATGCCCCGGGATAGTCCAGTGGCGATGTGGACCCCCAGAGGGGAAGAAGAAGACCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGgcgc < 1:307794/140‑1 (MQ=255) aaGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGCGGAAGAAAAAGATAGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCTTTTTAGCCGAAAGAACTGGTGGCGCGAAt > 2:278050/1‑143 (MQ=255) gcgcGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGGATCa > 1:836486/1‑143 (MQ=255) gcgAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCaaa < 1:690888/143‑1 (MQ=255) gcgAGTCGATGACCCGGGATATCCCCGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCaaa < 1:363933/143‑1 (MQ=255) aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGACGGAGCTGGTGGCGCGAATCAAAGCg > 2:65340/1‑143 (MQ=255) gTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCgg > 1:752444/1‑143 (MQ=255) gTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCgg < 2:475920/143‑1 (MQ=255) aTGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAAt < 1:716804/143‑1 (MQ=255) aTGACCCGGGAGATTCCAGTGGTGATGTGGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCGGGTGGCGGGAGTGAAAGCGGGaaa > 2:138226/1‑142 (MQ=255) cgcGGGATGTGCCGGTGGTGAGGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGCAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATgcgc < 2:275079/141‑1 (MQ=255) ggggATATGCCGGTGGTGATGTGGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTGTGCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCg < 2:353165/142‑1 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGACACCGGCGCGGATGACTATATCACCAAGCCGTTTTCTCCGCGGGAGCGGGTGGCGCGAATCAAAGCGCTAATGCCCCg > 2:97814/1‑143 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCg < 1:107412/143‑1 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGAGGACTATATCACCAAGCCGTTTTCGCCGAGGGAGCTGGTGGCGCGACCCAAAGCGGTAATGCCCCg > 2:599560/1‑143 (MQ=255) attcgggtggtgATGTTGACCCCCAGGTGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTc < 2:256818/137‑1 (MQ=255) ttCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCg < 1:553227/143‑1 (MQ=255) ttCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCg < 1:781960/143‑1 (MQ=255) ttCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCGGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTca > 1:647384/1‑142 (MQ=255) tCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATGTCGc > 1:136869/1‑143 (MQ=255) cAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCa > 1:414951/1‑143 (MQ=255) gtgATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGGGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGCCTCACAGCGGTAATGCGCCGAATTTCGCCAACGGc > 2:572546/1‑143 (MQ=255) tgATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTACGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCg > 2:433688/1‑143 (MQ=255) aTGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGCAACCGGCGCGGATGACTATATCCCCAAGCCGGTCTCGCCGAAGGCGCGGGTGGCGCGAACAAAAGCGGCAACGCGCCGGAATTCGCCAACGGAggg > 2:659971/1‑142 (MQ=255) gTTGACCCCCAGAGGCGAGCAAGAAGATCGCGCGTGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTgg < 1:576256/143‑1 (MQ=255) ttGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATAACCAAGCCAAAATCGCAGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGa > 1:331580/1‑143 (MQ=255) | CTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGA > NC_000913/417254‑417527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |