| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 31.7% | 28.7 / 26.5 | 41 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (17/11); new base T (7/6); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913/4295920‑4296200 | aggtgcttACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc < 1:169370/137‑1 (MQ=11) ggCGTTTATGCCGCATCCGATATCAACGCCTGCTGCGACGCTTAACGCGTC‑ATATCAGGCCTACGCCAGCCAGCGCAATAGCCTGCT‑TTAGCGGGATTTTGTAGGTCGGGTAAAGCGTTTACGCCGCATCCGACATCAACGcc > 2:273068/1‑143 (MQ=11) ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc > 2:517294/1‑143 (MQ=17) gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGTT‑TTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGGCGCATCCGACTTCACTGCCt > 1:260693/1‑143 (MQ=11) gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGAAATAGCCTGAT‑TTAGCGTGCTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcat > 1:273724/1‑141 (MQ=12) gTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTACGCCGTCGTTGACA‑TCCTACGCAAGGGAG‑TCAGGCGTCCGTTAGTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa > 1:242172/1‑143 (MQ=2) tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt < 1:487821/143‑1 (MQ=32) ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg > 2:278379/1‑143 (MQ=32) tGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa < 1:123322/143‑1 (MQ=17) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg < 2:81651/141‑1 (MQ=17) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg > 1:81651/1‑141 (MQ=17) cGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTC‑GTATCAGACCTACGCCAGACAGCGCAATAGCCTGGT‑TTAGCGGGATTTTATAGGACGGATAAGGCGTTTACGCCGCATCCGACATCAACACCAGATGGGACga > 2:169370/1‑142 (MQ=11) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATAAAGCCTACGCCAGACAGCGCAATATCCTGAT‑TTATCGTGATTTTGTAGGCCGGATAAGGCGTATACGCCGCATCCGACATAAATGCCTGATACgcagc > 2:180403/1‑139 (MQ=11) gCATCCGACATCAACGCCTGATGCGACGATTAACGCGTC‑TTCTCAGGCCTACGCCAAACAGCGCAACAGCCTGAA‑TGAGCGTGATAATGTAAGTCGGATAGAGCATGTACGCCGCATCGGACAGACACGCCTGCAGCGACGcc > 2:45842/1‑142 (MQ=2) gACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg < 2:80419/142‑1 (MQ=25) gACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg > 1:80419/1‑142 (MQ=35) acgtCAACGCCGGCCGCGACGCGTGCCGCGTC‑TTATCAGGCCTACGCCCGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg < 2:247389/140‑1 (MQ=11) cagcAACCCCGGATGTGAAGCTTAACGCGGC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt < 1:146936/140‑1 (MQ=11) aTCAACGCCTGATGCGACGCTTACCGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 2:523897/143‑1 (MQ=21) aTCAACGCCTGATCCGACGCTTGCGGCGTC‑TTGTGAGGCCTACGCCAGACGGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc < 2:446536/143‑1 (MQ=11) cTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCCTCTCAgg > 2:154928/1‑143 (MQ=255) tGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAA‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACGCCTGATGCGAAGCTTGCACCGTCTTATCAGGc > 2:45462/1‑143 (MQ=11) tataAGGCCTAGGCGGTAGAGCGCATTAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGATGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 2:449002/143‑1 (MQ=11) tATGAGGCTTAAGCCGGACGGCGCAATAGCCTGAG‑TTAGCGTGATTTTGTAGGTAGGATTGGGGGCTGACGCCGCATCCGACGTCAACGCCTGATGCGATGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 2:182185/143‑1 (MQ=14) cAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc > 1:348739/1‑85 (MQ=35) cTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCGGGGTGTACTCACGCCTACGCCCGCCGGCGCTTTAGCCTgcgtca > 2:369040/1‑138 (MQ=11) tACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCTAGCCAGCGCAATAGCCTGaggaag > 2:399806/1‑138 (MQ=255) aGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg < 1:481529/74‑1 (MQ=35) aCAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGCTTTAGCGTGAgtt > 1:367036/1‑143 (MQ=255) cAGCGCAATAGCCTGAC‑TTAGCGTGATTTTGTACGTCGGATAAGGCGTTTACGCCGGATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt < 1:84870/143‑1 (MQ=255) cgcAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGa < 2:445160/78‑1 (MQ=255) cgcAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa < 2:142390/143‑1 (MQ=37) aGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATGCCAGCCAGCGCTATAGCCTGATTTAGCGTGATTCTCTAGGTcag > 2:474335/1‑141 (MQ=11) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAAACCCTGAATAGCCGTCATTTTGTAGATCGGCGAACGCGCTTAGGcc > 2:298470/1‑143 (MQ=11) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGACTTTGTAGCTCGGATAAGGCGGGTATGCCGc > 1:70015/1‑143 (MQ=14) ggTCGGATAAGGCGTTTACGCAGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGACTCTGTAGATCGGACAAGGCCTTCACGCCCCATCCGa > 2:318349/1‑143 (MQ=11) tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAcc > 1:146290/1‑142 (MQ=38) ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATGAGGCGCGTATGCCGCATCCGACATCAACGcc > 1:263486/1‑143 (MQ=255) cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGc > 1:396637/1‑143 (MQ=35) ctggttgaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCGTCAGCCAGCGCCATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAGGGCGTGTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcg < 2:132491/136‑1 (MQ=11) tgtctaCGCCTGATGCGCCGCGTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt < 2:427124/138‑1 (MQ=255) aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTCACCCCGTCttc > 2:101569/1‑142 (MQ=11) | AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC‑TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT‑TTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913/4295920‑4296200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |