| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 91.7% | 37.4 / ‑1.9 | 12 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (5/6); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 91.7% | 37.1 / ‑2.4 | 12 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (5/6); minor base T (0/1); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGA > NC_000913/2173222‑2173479 || tttCCTCGATGCACTGCGACCGCCATTGCCACGAATATGTCTATTGTTGTTAGATCGCCAAACGGCAGCACCTGATAACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc < 1:290019/143‑3 (MQ=255) tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa > 1:271889/1‑143 (MQ=255) gcgtcACGAAGAAGCCGATGCTGGTAAGATCGCCAAACGGCAGCACCTGATTACCCGCCACACAAACAGCAATTATAATGGTGAGTGGTATAAAAATCAGGCTTGCCGATACCA‑‑CCGCAGTATGTCCCAGCAGCAACGCCGGa < 2:127253/139‑1 (MQ=255) ccACGAAGGAGCCGATGGTGGCAAGATCGCTAAACGGCATCACCTTATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCa < 1:207916/143‑1 (MQ=255) aTGTTGGGAAGAGCGTCAATCGGGAGCATCTGATTAGCCGGCATACAAACAGTAATTAAAATGGTTAGTGGTATATAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCTCAGCAGCAACGCCTGTTCAAGGCCAATCAGGaa < 2:386218/143‑1 (MQ=255) gATTACCCGGCACACAAACAGCAATTAAAATGTTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGACGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa < 1:71325/143‑1 (MQ=255) acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATTAAGGCTAATCAGAAACTCCTGACAGCCGAACTTCGCCTGTACACCACTACGAGCCTg > 1:23255/1‑143 (MQ=255) gtcaaaTGGTGAGTGTGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCCGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACTACTACGAGCCTGCTTAGCGATGGGc < 2:128278/140‑1 (MQ=255) aaaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCACCGTATGTACCAGCAGCAACGACGGCTCAAGGCCAATCAGGGACTCCTGACCGCGAAACTTCACCTGTACGCGACTACAAGACAGCTTACCAGTGGGcct > 2:357026/1‑141 (MQ=255) tGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTATAAGGAAAAAAAACAGCTTAGCAATAGGCGTTAAAcaa > 1:255037/1‑141 (MQ=255) gcgttgtaTAAATTTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAGCAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat < 1:59320/136‑1 (MQ=255) tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCTCGAAGAAACGCCGGATGAAGGCCAATTGGGAAATACTGACTGCCGCACTTCGCCTGTAAACGAAAACGAGCCTGATTAGCAATGGGCGTTAAAccatcca > 1:146449/1‑143 (MQ=255) gATAAAAATCTGTCTTGCCTTTAC‑‑CTCCTCCTTATGTTCCATCAGTAACGCCGGATCAAGGCCAATAAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga < 1:68904/143‑1 (MQ=255) || TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGA > NC_000913/2173222‑2173479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |