Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	32 (1.600)	23/284	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	38 (1.900)	23/284	0.0	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979621‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890                                                                                                                                                                                                                                                                                         ttcctGTGATCATATAATTTCAAAGGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGTTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGT                                                                                                                                          <  2:50896/138‑1    CGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTATCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑G                                                                                                                                      >  2:359657/1‑143           CATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAATTTATTGGTTAATATATGTAAATCATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGC                                                                                                                               <  1:52842/143‑1            ATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTCACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCG                                                                                                                              >  1:73314/1‑143            ATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAAATAGGGAAGGTGC‑GAATAAGCG                                                                                                                              <  1:246725/143‑1                       AATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATGGTTGCCTTTCTCTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTT                                                                                                                   <  1:8183/143‑1                              TTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTACTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCT                                                                                                            >  1:288821/1‑143                                ATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGA                                                                                                          >  2:34412/1‑143                                                caGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGACTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGACTGACTCAGTCATTTCATTT                                                                                          <  2:127619/141‑1                                                 TGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAATTATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTGATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTC                                                                                         <  1:66946/143‑1                                                       tgcgtATTAATCATGTTGTTAATGTTTGTAAAATATTTCATTAACATTAAGTTGATTGTTGTCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGTGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATG                                                                                   <  2:384262/138‑1                                                       TCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATATCcacagg                                                                                   >  2:327266/1‑137                                                        CCGCATTAAACTAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGTGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGT                                                                                  <  2:195809/143‑1                                                            ATTAAACTAGTGGTTAATATATGTATATCATTTCATTAACATTAAGTTGATTTTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA                                                                              <  2:290744/143‑1                                                            ATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA                                                                              <  1:234348/143‑1                                                                                            TCATTAACATTAAGTTGATTGTTGCCTTTCTTTTTATTTTATTTTGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATC                                              <  1:87148/143‑1                                                                                               TTAACATTATGTTGATTGTTTCTTTTTTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGC                                           <  1:185835/143‑1                                                                                               TTAACATTATGTTGATTGTTGCTTTTCTTTGTATTTAATTAGGGAAGGTGC‑GATTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGC                                           <  2:37916/143‑1                                                                                                         GTTGATTGGTGCCTTTCTTTGTATTTGATTA‑GGATCCTGCTGAATTGGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAC                                 <  2:265899/143‑1                                                                                                         GTTGATCGTGGTCTGTCTTTGTATTTAATTAGGGTAGGTGC‑GAATTAGCTGGCAAATTCTTCTCCGCCTGCTCAGTCATTTCATTTCTTCATGTTTGAGCCGTTTTTTTTTCCCGTAAATGCCTTGAATCAGCCTATTTAGAC                                 <  2:267550/143‑1                                                                                                           TGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCTTAAATGCCTTGAATCAGCCTATTTAGACCG                               >  2:51543/1‑143                                                                                                               TGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATTAGCGTGTAAATTTTTCTCGTTTGTTTCTGTCATTTCATTTCTTCAGGTTTGAGCCGATTTGTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTC                           <  1:90574/143‑1                                                                                                                 TTGCCTTTCTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTGCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTT                         <  2:302995/143‑1                                                                                                                         CTTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGCCTGACTCAGTTATTTCATTTCTTCATGTTTGAGACAATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTTTTAGCCATTT                 >  1:51134/1‑143                                                                                                                          TTTGTATTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGCCGATTTTTTCTCCAGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA                >  1:144425/1‑143                                                                                                                                TTTAATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGT          >  1:196003/1‑143                                                                                                                                   AATTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTCCGCCATGTAAGGCGacat       >  2:310394/1‑139                                                                                                                                     TTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGTCTATTTTTGCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC     <  2:127616/143‑1                                                                                                                                     TTAGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTTTCTTTTATATTCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCTATTTTAGGCGTTATCC     >  2:206166/1‑143                                                                                                                                       AGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAATGCGTTATCCCC   >  2:96391/1‑143                                                                                                                                       AGGGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCC   >  1:179128/1‑143                                                                                                                                        GGGAAGTTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGTCTCAGTTATTTCATTTCTTCATGTTTGTGCCGAATATCTCTCCCGTAGTTGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  1:213609/143‑1                                                                                                                                                                                                                                                                                         TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979621‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGC‑GAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.