| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 97.8% | 137.3 / ‑4.0 | 45 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (34/10); total (35/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC > NC_000913/3560316‑3560589 | gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGCGTTCCTGGATGACGAGGGCAGCGCCGCACCGCCATGATGG‑cgca > 1:134226/1‑140 (MQ=255) gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CCGGtt > 1:17323/1‑143 (MQ=255) gAGCCATTGGGGATTTGCTCCGCGACGTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCCGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CCGGcttcg > 2:445332/1‑141 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTAGGTCTGGGTGGACTTGCACACGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAAGAT‑CGGGTTTTg > 2:638038/1‑143 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCATGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTc > 1:239881/1‑143 (MQ=255) gttGGGGAGTCGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCg < 2:532600/142‑1 (MQ=255) ggtatctgctgcGCCCTTTCGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAg < 2:299157/137‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGCCAGCGCCGCACCACCAGGATGG‑CGCAGGAG‑CAGGTTGTGCGCCGCCAGcc > 2:301489/1‑142 (MQ=255) tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGCT‑CAGGGTTTGCTCCGCCCGCTCa > 2:683962/1‑143 (MQ=255) tGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGGCCGCtgcgt > 2:296367/1‑139 (MQ=255) gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCATGATGG‑CGCGGGATGCAGG‑TCTGCTCCGCCAGCTCATTg > 2:287215/1‑143 (MQ=255) cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTATTAACCGAACTGAAAGACAGCGCGGCACAGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGa > 1:458820/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAg > 1:555290/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGCTTTGCGCCGCCAGCTCATTgcg > 2:623089/1‑141 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTATGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCC‑CAGGAT‑CAGGAATTGCACCGCCAGCTAATTGaa > 2:39591/1‑142 (MQ=255) ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGCGTGGCCTTGCGATCGTGCCACGGAGTGTTAACCGAACGGGAAGGCAGCCCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGGATTGCACCGACCGCCCATAGacg > 2:675326/1‑141 (MQ=255) gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGGT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTc > 1:471629/1‑143 (MQ=255) ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg > 1:372587/1‑143 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa > 2:150015/1‑143 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGAGCAGCGCACCACCGGCAGGGTGG‑CGCAGAGT‑AAGGCTGTTCACCGCCAGCCCATGAAGGACGCGGCGGACGGTCTGCGGGca > 2:80969/1‑142 (MQ=255) cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag > 1:617494/1‑143 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGGCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTACGGCCTGACGgaga > 2:155951/1‑143 (MQ=255) gTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CATCTGGGTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc < 2:481753/143‑1 (MQ=255) ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc > 1:486425/1‑143 (MQ=255) gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGC‑CGCCGGAA‑CAGGTTTTGCTCCGCCAGCCCACTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCCCTACCAGcacgtag > 2:306845/1‑137 (MQ=255) tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt > 1:342466/1‑143 (MQ=255) cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 1:184832/143‑1 (MQ=255) gTGGCACGGCGTGTGAACGGAACTGGGAGGCAGCGGCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc < 2:698323/143‑1 (MQ=255) tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTCCGGTCCt > 2:394334/1‑143 (MQ=255) gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa > 2:8957/1‑143 (MQ=255) gtggcccGAGCGGGCAGGCAGCGGGGCACCGCCAGGGTGG‑CGCAGGAG‑CAGGTTTTGCTCCGCCTGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct < 2:591804/138‑1 (MQ=255) aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCGGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct > 1:668591/1‑143 (MQ=255) aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgca > 2:130927/1‑142 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGAGAATAGTCGTCGGGCTGACGGAGACATGCACTAACAGCTATTCGGGAATGACACAAGCctgctggt > 2:439420/1‑141 (MQ=255) gAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTGCTGACATAACCCTGCTGgtt > 1:450981/1‑143 (MQ=255) cTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCACTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:471629/143‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc < 1:150015/143‑1 (MQ=255) gAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGCCTGACGGAGAAATGCTCTACCAGCTCTTCGGTGCTGACATAACCCGGCTGTTTAACCAg > 2:360680/1‑143 (MQ=255) aGGCAGCGCCGCGCCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCCGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 2:258732/143‑1 (MQ=255) ggCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtgc > 1:592302/1‑141 (MQ=255) ggCAGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAGTGCGCTACCAGCTCTCCGGTACTGACATAACGCTGCCGTCTAACCAgacc > 2:207864/1‑140 (MQ=255) aGCGCCGCACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATGACCCTGCTGTTTAACCAGTTCGat > 1:560928/1‑143 (MQ=255) cACCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg < 2:17323/143‑1 (MQ=255) aCCGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt > 1:372956/1‑143 (MQ=255) cGCCATGATGG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTACTGACTAACCAGTTCGAGAATACCgctc > 2:671892/1‑140 (MQ=255) atgatgG‑CGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATACTACCGTTGTGAc > 1:632309/1‑143 (MQ=255) | GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC > NC_000913/3560316‑3560589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |