| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 701,378 | +T | coding (217/1221 nt) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 701,378 | 1 | . | T | 83.8% | 84.3 / 12.7 | 38 | coding (217/1221 nt) | nagC | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (4/2); new base T (14/17); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG > NC_000913/701243‑701501 | gCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑GTG‑C‑C‑CCCCGt > 2:665661/1‑143 (MQ=255) tAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCct > 1:281746/1‑143 (MQ=255) ttgttctGCCACCCCTTTGCTGCGCTGATCACACAGCGTGATGGTGGCGTCATGACGCCCGCTCCGTACGCCGCTTGCGTCGTCA‑TTGCAGGTTTCGGTGACGATGGATATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGa < 2:714238/140‑1 (MQ=255) tctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCaa < 1:372895/143‑1 (MQ=255) ccAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAActtc > 1:258898/1‑143 (MQ=255) ttttGCTGCTGAGATCAAACAGAGCGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt < 1:3929/142‑1 (MQ=255) cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt < 2:304622/143‑1 (MQ=255) tttGCTGCTGAGATCAAACAGAGTGAAGCTAGCGTCATGTCGACCAAGCCGTACGCCGATTGCGAGGAAAGTTGC‑GGATCGGAGGACGAGGGAGATAGCGCGAC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGAGCAACGTCTGTGATc > 2:398454/1‑143 (MQ=255) tgctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGccc > 1:213733/1‑143 (MQ=255) gaTCAAACTGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTc < 1:708555/143‑1 (MQ=255) aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGACA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTCCg > 1:322690/1‑143 (MQ=255) aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCg > 1:107721/1‑143 (MQ=255) tCAAACAGAGTGATGGTGGCGTCATGACGACCAAGACGTAAGCCGATTGGGTGGAAA‑TTGCGGGTTTCGGTGAAGATGGAGATAGCGCGGC‑G‑G‑CAT‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGCTCAGCGCGCGCTCGa > 2:557497/1‑143 (MQ=255) aCAGAGGGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTGTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAg < 1:35673/143‑1 (MQ=255) gtggtggtgGCGTCGTGAGGGCCAAGCTGTGGGGCGATTGGGTGGAGA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAc < 2:458038/143‑1 (MQ=255) tggtggtggGGTCAGGCCGGCGCAGGCGTACGCGGAGTGCGTGGAAA‑GTGCGGGTTTCGGTGACGAGGGAGATAGCGGGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGatg < 2:650250/140‑3 (MQ=255) tGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑GCC‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATACGCTGAcg > 1:583283/1‑143 (MQ=255) aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGGTCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAcgcg > 2:407501/1‑143 (MQ=255) tCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg > 2:94261/1‑143 (MQ=255) cATGACGGCCAAGCTGTACGCCGATGGTGTTGAGA‑GTGCGGGTTGCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGCTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGt < 2:41970/143‑1 (MQ=255) aTGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGAC‑G‑G‑C‑CTCCCGGTGGAGGCCGGCTGATCAACTACTTTGATAAGCCCCGGTACGATAAGACTACGCGAAAATCTGGtg > 2:611474/1‑142 (MQ=255) tGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑G‑C‑T‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTGCGGTAGGCTGACGCGTCATTTTGGTTa > 2:39472/1‑143 (MQ=255) tGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTa > 1:22494/1‑143 (MQ=255) gacgacCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTAc < 1:477632/143‑1 (MQ=255) cccaGCCGGCCGCCGACTGCGTGGCAA‑GTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTgg < 2:419853/140‑1 (MQ=255) aaGCCGTACGCCGATTGCGGGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGAGCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgg < 1:214642/143‑1 (MQ=255) aGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgg < 1:667587/143‑1 (MQ=255) aCGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCaa > 1:331442/1‑143 (MQ=255) cGCCGATGGCGTGGAAG‑TTGCGGGTTTCGGGGACGGTGGAGATAGCGCGGC‑G‑G‑CGT‑CCCGGTGGAGGCCCGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAg < 1:399891/143‑1 (MQ=255) gATTGCGTGGGAA‑TTGCGGGTTGCGGTGGCGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATGAGCGCGCGTTGGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTgg < 2:538698/143‑1 (MQ=255) gTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTCCGCTGGCGGGGGCAAGATGGCTTTGc > 2:110514/1‑143 (MQ=255) gaca‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCg < 1:236452/140‑1 (MQ=255) acgtggCGGGTTCCGGTGACGTTGGAGATAGCGCGGC‑C‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCGTTGATCAGCCCGCGTTCGACAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCACCCTGGCTGGGCTCgg < 2:525112/138‑1 (MQ=255) gCGGGTTTCGGGGACGATGGAGATAGCGCGGC‑G‑G‑C‑CGCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATc < 2:8962/143‑1 (MQ=255) tGACGGTGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCGGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCga < 2:258898/143‑1 (MQ=255) gATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTGCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTGCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGGtt > 2:633926/1‑143 (MQ=255) aTGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTg < 2:107721/143‑1 (MQ=255) tGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATggg > 2:495192/1‑143 (MQ=255) | GCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG > NC_000913/701243‑701501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |