Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,306	C→T	R149H (CGT→CAT)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,306	0	C	T	100.0%	222.5 / NA	72	R149H (CGT→CAT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/41);  total (31/41)

GTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATC  >  NC_000913/702167‑702442                                                                                                                                            |                                                                                                                                         gTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGca                                                                                                                                       <  1:333473/143‑1 (MQ=255)      cGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGTCACTCGACCAGCGCGGCATCAGGCTTATGcacacac                                                                                                                                  >  1:526661/1‑140 (MQ=255)      cGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACacaa                                                                                                                                  >  1:370147/1‑143 (MQ=255)       ggCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTCTGCACcaact                                                                                                                                 >  1:432413/1‑141 (MQ=255)         cAGACACCACGATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCACTTCCGGTGCCAGGGTCACGTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTc                                                                                                                               <  1:704289/143‑1 (MQ=255)           gAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGCCACCTTGGTTATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCgg                                                                                                                             <  1:1062067/143‑1 (MQ=255)            aaaCCACAATCCCGGCATTTGCCAGTTTGCTGATGACGTCCGCAGGAACCATTTCCGGTGCCAGGGTCACGTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGa                                                                                                                            <  1:137784/143‑1 (MQ=255)                cacaATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACGACAATCGAATTac                                                                                                                        >  2:1014458/1‑142 (MQ=255)                  cagtCCGGGCATTTGCCAGTTTGCTGATGACTTCCGGAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATgg                                                                                                                      <  2:771426/140‑1 (MQ=255)                  caATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATgg                                                                                                                      >  1:508477/1‑143 (MQ=255)                    gtCCCGGCACGTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGt                                                                                                                    <  2:499493/142‑1 (MQ=255)                     tCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAACATTCGGCTTATGGGTg                                                                                                                   >  1:40120/1‑143 (MQ=255)                      cccGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGc                                                                                                                  <  1:580576/143‑1 (MQ=255)                       ccGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGcc                                                                                                                 >  1:63754/1‑143 (MQ=255)                           cATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGAGTCACGGTGCCtatg                                                                                                             >  2:561811/1‑140 (MQ=255)                                 ccAGTTTGTTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGTGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTAcc                                                                                                       <  2:278143/143‑1 (MQ=255)                                        gCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCa                                                                                                <  2:762047/143‑1 (MQ=255)                                         cTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAg                                                                                               <  1:575960/143‑1 (MQ=255)                                         cTGATGACGGCCGCAGGCACCCGTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTCTCACACAGGAAATCGACCAGCGCGGCATGAGGCTTATGCACAAAGTGCGGATTATGGGTGCCTTTTTTTACCAGATTCAg                                                                                               <  1:1129426/143‑1 (MQ=255)                                          tgatgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGc                                                                                              >  2:501912/1‑143 (MQ=255)                                             tgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATCATGGGTGCCTTTTTTTACCAGATTCAGCCAc                                                                                           >  2:1185954/1‑143 (MQ=255)                                                cTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCAGACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGa                                                                                        <  1:991566/143‑1 (MQ=255)                                                     gCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTc                                                                                   <  2:63754/143‑1 (MQ=255)                                                      cAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAAGGACGTCGGCGTTTTCACACAGGAAATTGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTcc                                                                                  <  1:1104258/143‑1 (MQ=255)                                                       aGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGCGTGCCTTTTTTTCCCAGATTCAGCCACGGACCtcgca                                                                                 >  2:41619/1‑139 (MQ=255)                                                        ggAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAACATTCGGATTATGGGTGCCTTTTTTTACCAGCTTCAGCCACGGACCTTCCAg                                                                                >  2:300237/1‑143 (MQ=255)                                                          aaCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGAt                                                                              <  1:535717/143‑1 (MQ=255)                                                           aCCATTTCCGGTGCCCGGGTCACTTTGGTACTGACGTCGGCGTTTTCACTCAAGAAATCTACCAGCTCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATg                                                                             <  1:12092/143‑1 (MQ=255)                                                            ccATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATga                                                                            >  1:583709/1‑142 (MQ=255)                                                                     gTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAAc                                                                   >  2:992630/1‑143 (MQ=255)                                                                       gCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGc                                                                 <  2:160272/143‑1 (MQ=255)                                                                          aGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTGCCAGATTCTTCCCCGGACTTCCCCGATGCATACCTAAccactg                                                              >  2:399047/1‑138 (MQ=255)                                                                           gggTCAGGTGGGTAATGACGGCGGCGTCTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGa                                                             <  2:1016795/143‑1 (MQ=255)                                                                            ggTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACACAATTCGGATTATGGGTGCCTTTTTTTACTAGATTCAGCCACGGACCTTCCAGATGCAGACCGAACGcgtcat                                                            >  2:634282/1‑138 (MQ=255)                                                                            ggTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTAGGGGTGCCTTTTTTTACCAGATGCAGGCACGGGCCTTTCAGCTGCAGACCTAAAGCCTgct                                                            >  2:1066077/1‑141 (MQ=255)                                                                             gTCACTTTGGTAGTGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGTATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGAtt                                                           <  2:136592/143‑1 (MQ=255)                                                                              tCACGTCGGTAATGACGTCGGCGGTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTc                                                          <  1:866726/143‑1 (MQ=255)                                                                                  tttGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATGAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCGGATGCTGCCCTATCGCCTGCTTCGGAt                                                      >  1:45908/1‑143 (MQ=255)                                                                                     ggTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGAACTTCCAGATGCAGACCTAACGCGTGATTAGGATGtt                                                   >  1:292042/1‑143 (MQ=255)                                                                                         aTGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGc                                               <  1:562855/143‑1 (MQ=255)                                                                                         aTGACGTCGGCGTTTTCACACAGGAAAGCGACCAGCGCGGCATCAGGCTTATCCACAAAATTAGGTTTATTAGTGTCTGTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGc                                               <  2:840574/143‑1 (MQ=255)                                                                                          ttaCGTCGGCTTTTTCACACAGTAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCACATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTAcc                                              <  2:831525/141‑1 (MQ=255)                                                                                          tGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGcc                                              >  1:354714/1‑143 (MQ=255)                                                                                            aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAg                                            <  1:992630/143‑1 (MQ=255)                                                                                            aCGTCGGCGTTTTCACACAGGAAAGCGACTAGCGTGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCATTTTTTTACCAGAGTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAg                                            <  2:1008808/143‑1 (MQ=255)                                                                                              gTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAACATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCATGt                                          >  1:426411/1‑143 (MQ=255)                                                                                              gTCGGCGTCTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGt                                          <  2:358968/143‑1 (MQ=255)                                                                                                   cgattccacacaGTCAGTCGACCAGCTCGGCTTCATGCTTATGCACAACATTCGGATTATGGGTGCCTTTTTTTTCCAGCTTCAGCCACGGACCTTCCAGATGCCGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcg                                     <  2:159478/137‑1 (MQ=255)                                                                                                   cGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCACATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcg                                     <  2:530241/143‑1 (MQ=255)                                                                                                   cGTTTTCACACAGGAAAGCGACCAGCGCGGCATCAGGCTTATGCACAAAAATCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcg                                     >  2:911422/1‑143 (MQ=255)                                                                                                         cacacaGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATa                               <  1:501912/143‑1 (MQ=255)                                                                                                          ccacaGGAAATCGACCAGCGCGGCATGAGGCTTATGCACAAAATTCGGACTATGGCGGCCTTTTTTTACCAGATTCAGCGACGGACCTTCCAGATGCAGCCCTTACGCCTGCTTCGGACGTTTTGCCACGTACCCgcgcctca                              >  2:1131373/2‑139 (MQ=255)                                                                                                          acacaGGAAATCGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATaa                              <  1:317344/143‑1 (MQ=255)                                                                                                              aGGAAATAGACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGGTTTGCCAGGTAGTCGCGCATAAcgcg                          >  2:1059467/1‑143 (MQ=255)                                                                                                               ggATATCTACCCGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACgcgc                         <  1:1110469/143‑1 (MQ=255)                                                                                                                     cGACCAGCGCGGCATCAGGCTTATGCACAAAAGTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGccc                   <  2:370147/143‑1 (MQ=255)                                                                                                                      gACCAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCt                  >  1:1038603/1‑143 (MQ=255)                                                                                                                        ccAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGTTGTTTTGCCTGGTCCTCGCGCATAACGCGCACGCCCTGt                >  1:658669/1‑143 (MQ=255)                                                                                                                        ccAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGt                >  2:48721/1‑143 (MQ=255)                                                                                                                         cAGCGCGGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGtt               <  1:1106486/143‑1 (MQ=255)                                                                                                                          aGCGCGGCATCAGGCTTATGCACAACCTTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGttt              <  2:662136/143‑1 (MQ=255)                                                                                                                            cgcgGCATCAGGCTTATGCACAACATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGCTGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtca            <  2:508477/143‑1 (MQ=255)                                                                                                                            cgcgGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACAAAGAGATATTAACGGTTGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtca            >  1:545882/1‑143 (MQ=255)                                                                                                                             gcgGCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcat           <  1:1142970/143‑1 (MQ=255)                                                                                                                              cgGCAACAAACTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTACCGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatc          <  2:763113/143‑1 (MQ=255)                                                                                                                                gCATCGGGCTTATGCACAAGCTGCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAGCGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATATCGCGCACGCCCTGTTTCATCAg        <  2:92577/143‑1 (MQ=255)                                                                                                                                gCATCAGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCGCGCCCTGTTTCATCAg        >  1:506232/1‑143 (MQ=255)                                                                                                                                gCATCAGGCTTATGCACAAAATTCGGACTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAg        <  1:48721/143‑1 (MQ=255)                                                                                                                                 cacctggCTTATGCACAACATTCGGATTATGCGTGCTCTCTTTTACCAGACTCCGCCACGGACCTTCCACATACAGACCTACCGCCTGATTCGGATGTTTTGCCAGGTACTCGTGCATAACGCGCACGCCCTGTTTCATCAGc       <  2:90001/138‑1 (MQ=255)                                                                                                                                     aGGCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGCCCTTCCAGATGCAGACCTAACGCCTGCTTCGGCTGTTTTGCTAGGTCCTCGCGCCTAACGCGCACGCCCTCTTTCCTCAGCTcct   >  2:852249/1‑141 (MQ=255)                                                                                                                                     aGGCTTATGCACAAAATTCAGATTATCCGAGCCTTGGTTTACCAGATTCAGCCACGGACCTTCAAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCTCATACCGCTCACGCCCTGTTTCATCAGCTCAt   >  2:256494/1‑143 (MQ=255)                                                                                                                                      ggCTTATGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATc  <  1:449459/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         GTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATC  >  NC_000913/702167‑702442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑