| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.5% | 118.1 / NA | 41 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (22/17); minor base T (0/1); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT > NC_000913/2132648‑2132922 | gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct > 1:212460/1‑143 (MQ=255)gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct < 2:180759/143‑1 (MQ=255) tAGAGGGTAAACAGCTGTTATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCTGCAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:97128/143‑1 (MQ=255) gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct > 2:550277/1‑143 (MQ=255) tgTACACAGCTGATATTTTTTGCTGGCGCGGTTGAATTGGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTT‑CGCTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 2:421223/142‑1 (MQ=255) ggTCAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 2:168967/143‑1 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAAGTGATCTGCATTTCACCGTGACTGATATGCAGGCACTGCGTCGCcactc > 2:41611/1‑142 (MQ=255) aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 2:335987/143‑1 (MQ=255) cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc > 2:228215/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa > 1:540717/1‑143 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTGG‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg < 1:494806/143‑1 (MQ=255) atTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGCTGGAGATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGt > 1:566077/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 1:84346/1‑143 (MQ=255) tttgGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg < 2:195142/143‑1 (MQ=255) tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGGTCTGCGTCGCCTCTTCTCCTTTCGACGGttag > 2:325357/1‑141 (MQ=255) ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACAGTCATTGATATGCAGGCCCACCGTACGCTCTTTTACTTTCCACGGTACGcc > 1:319927/1‑143 (MQ=255) cggccggggTCGACTTGGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGTGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATTTGCGTGTTCTTGCTCCCCACCACTACTTTCCACGGTTCGCCGTa < 2:707864/136‑1 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 2:390809/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa > 1:466911/1‑143 (MQ=255) ttGCGGTAAAAGTGAAAGTACCCTGAGAATCTTTT‑CGGTGACGAGGCGATCTGCATTTCACCGTGATTGATATGCAGGCTATGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 1:843882/143‑1 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAAACATCCTCAAGAAGATGTCGTaa > 1:555770/1‑143 (MQ=255) tAAACGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 1:294689/143‑1 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAGGCTGTCGTAATc > 1:247472/1‑143 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTaacc > 2:274865/1‑141 (MQ=255) aaGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg > 2:392501/1‑143 (MQ=255) ggAAGCACCCGGAGAATTTTGT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc < 1:133814/143‑1 (MQ=255) ccGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCCGGAGGATGTCGTAATCCTGTGCCGCTTTccg > 1:34890/1‑141 (MQ=255) cGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt < 1:228215/143‑1 (MQ=255) ggAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCTTCGTCCTTTGCCTCTTTCAttt > 1:817385/1‑143 (MQ=255) gagaATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCGTACTCCCCCCCCCTCCGCAGGCAGATGTCGTAAGCCTGTCCCGCCTTCCGtcc > 2:580835/1‑141 (MQ=255) tgtttttt‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt < 2:80023/141‑1 (MQ=255) tttttt‑CGTTTATGAGGTGATCTGCATTTCATCGTTATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt < 2:69111/143‑1 (MQ=255) tt‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:550277/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:795844/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:84346/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCGACCACCATCCGCGGGAAGATGTCGTAATCCTGTGCCGCTTTCCGCTCGGTCTAGagcaaa > 2:146525/1‑143 (MQ=255) aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTACTCCACCACCATCCGCCGGCCGATGTCCGAGCCCGGTCCCGCGTTCGGTGCGGCCTCGAACCAacac > 1:93001/1‑143 (MQ=255) aGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCCTAATCCTGTGCCGCTTTCCGCTCCGTATCGCACACACActct > 2:681678/1‑143 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTCCAGATCGCTATCGAACAAACACTCtt > 2:31167/1‑143 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCGACAGCGCGGCATCGAACAAACACTCTATGac > 2:486502/1‑142 (MQ=255) tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt > 1:188266/1‑143 (MQ=255) ttgatAGGCAGGCGCTGCGTCGCCTCTGCTACTGGCCACGGTGCGCCGTACTCCACCAGCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGGAGCGAACAAACACTCGTGGAAACGCCATGCCCAGGTaaa < 2:166512/143‑1 (MQ=255) atGCAGGCTCTGCGTCTCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 2:367511/143‑1 (MQ=255) | GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT > NC_000913/2132648‑2132922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |