Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,726,876	C→T	P73L (CCT→CTT)	nemA →	chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,726,876	0	C	T	100.0%	114.6 / NA	38	P73L (CCT→CTT)	nemA	chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/18);  total (20/18)

GTCTGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAG  >  NC_000913/1726741‑1727012                                                                                                                                        |                                                                                                                                         ggcagcgcAGGAATGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATc                                                                                                                                   <  1:25755/139‑1 (MQ=255)          gTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCg                                                                                                                          <  1:652188/143‑1 (MQ=255)            aTTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGAGTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGa                                                                                                                        >  2:270292/1‑143 (MQ=255)             ttGAACCGGGTGACACTCCAACAAAGGAGGTGGCGGAAGACTATCGCCAACGTGCCAGTGCCGGTTTGCTTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCACGAGGATGTGCAGGTGCGCTTGTCATCCATAGCCCGGAg                                                                                                                       >  2:107631/1‑143 (MQ=255)              tGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGc                                                                                                                      >  1:629810/1‑143 (MQ=255)                  ccGGGTGACATTCCTGCCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAAt                                                                                                                  <  1:381327/143‑1 (MQ=255)                          cATTCCTACCCCGTTGATTGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCAt                                                                                                          <  2:2685/143‑1 (MQ=255)                              ccTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGaa                                                                                                      >  2:377532/1‑143 (MQ=255)                                  gcccGGTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGaaaaaa                                                                                                  <  1:565739/142‑1 (MQ=255)                                           gggCAGAATAATATCGACAACGGGCCAGTGCCGGTTTGATTACTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCt                                                                                         <  1:26435/142‑1 (MQ=255)                                             gCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTgg                                                                                       >  2:269182/1‑143 (MQ=255)                                                gAATACTATCGCCAACGTGCCAGTGCCGGTTTGCTTATTAGTGACGCCACGCAAATTTATGCCCAGGCAAAAGGATATGCAGGTGCGATTGGCATCCATAGTCCGGAGCAAATTCCCGCATGGAAAAAAATCACGGCTGGCgc                                                                                    >  2:115310/1‑142 (MQ=255)                                                      tatatccAACGTTCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAAGTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc                                                                              <  2:353840/138‑1 (MQ=255)                                                      tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc                                                                              >  2:610278/1‑143 (MQ=255)                                                      tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc                                                                              >  1:124205/1‑143 (MQ=255)                                                         cGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTgc                                                                           >  2:798873/1‑142 (MQ=255)                                                           ccAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAACAAAATCACCGGTGGCGTTCCTGCTGgaa                                                                         >  2:564872/1‑143 (MQ=255)                                                              aCGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATg                                                                      >  1:103228/1‑143 (MQ=255)                                                                 tGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTc                                                                   <  2:196375/143‑1 (MQ=255)                                                                     aGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCatat                                                               <  1:270292/143‑1 (MQ=255)                                                                       tGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAACGGTCATATgg                                                             >  1:176716/1‑143 (MQ=255)                                                                             tttGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGc                                                       >  2:684406/1‑143 (MQ=255)                                                                                       aGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGca                                             <  1:823916/143‑1 (MQ=255)                                                                                       aGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGca                                             >  1:159068/1‑143 (MQ=255)                                                                                               cACGCAGATGTCGGCCTGGGGAAAGGGCTATGCAGGGGCGGTTGGCATCCATAGTCGGGAGCAAATGGCCGTATGGAAAAAAATCACCGCGGGCGTGCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGAc                                     <  2:370432/143‑1 (MQ=255)                                                                                                aCGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACg                                    >  1:278210/1‑143 (MQ=255)                                                                                                      aTTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCTCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTtc                              <  1:772620/143‑1 (MQ=255)                                                                                                       tttCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCAGCATGGAAATAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTtct                             >  1:772838/1‑143 (MQ=255)                                                                                                               naGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGCGCAAATTGCCACATCGAAAAAAATCACCGCTGGCGTTCATGCTGAAGCTGGTCATATGGCCGTGCAGCCGTCGCCCCCCGGCCGCCTTTCTCAcccgcg                     >  1:164250/2‑138 (MQ=255)                                                                                                               cAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAg                     >  2:503084/1‑143 (MQ=255)                                                                                                                  ccattgggATATGCAGGTGCGCTTGGCAGCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCt                  <  1:190102/137‑1 (MQ=255)                                                                                                                   cAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCACTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTg                 <  1:377532/143‑1 (MQ=255)                                                                                                                    aaaaGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCCTTTCTCACGCCAGCCTGc                >  1:826663/1‑143 (MQ=255)                                                                                                                      aaGCATATTCCGGGGCGCTTGGCATCCATAGTCCGGATGAAATTGCCGAATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCaa              <  2:357918/143‑1 (MQ=255)                                                                                                                         gATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCt           >  1:419594/1‑143 (MQ=255)                                                                                                                             tGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCg       <  2:815471/143‑1 (MQ=255)                                                                                                                                 ggTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAAGCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACAGGCCGAATTTATCACGCCAGCCTGCAACCTGGCGGTCa   <  2:525178/143‑1 (MQ=255)                                                                                                                                  gTGCGCTTGGCATCCATAGTCCGGAGCAAACTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAg  <  1:296517/143‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GTCTGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAG  >  NC_000913/1726741‑1727012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑